Gene description for HIST1H2AI
Gene name histone cluster 1, H2ai
Gene symbol HIST1H2AI
Other names/aliases H2A/c
H2AFC
Species Homo sapiens
 Database cross references - HIST1H2AI
ExoCarta ExoCarta_8329
Vesiclepedia VP_8329
Entrez Gene 8329
HGNC 4725
MIM 602787
UniProt P0C0S8  
 HIST1H2AI identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Neuroblastoma cells 25944692    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
 Gene ontology annotations for HIST1H2AI
Molecular Function
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    structural constituent of chromatin GO:0030527 IBA
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    heterochromatin formation GO:0031507 IBA
Subcellular Localization
    nucleosome GO:0000786 IBA
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified HIST1H2AI in sEVs
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 138
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
6
Experiment ID 140
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
7
Experiment ID 141
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
8
Experiment ID 142
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
9
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
10
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
11
Experiment ID 146
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name RWPE - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
 Protein-protein interactions for HIST1H2AI
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 HIST3H3 8290
Cross-Linking-MS (XL-MS) Homo sapiens
3 RALY 22913
Affinity Capture-MS Homo sapiens
4 HIST2H2BE 8349
Cross-Linking-MS (XL-MS) Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 HNRNPC 3183
Affinity Capture-MS Homo sapiens
7 CUL1 8454
Affinity Capture-MS Homo sapiens
8 RPL26L1 51121
Affinity Capture-MS Homo sapiens
9 CBX1 10951
Affinity Capture-MS Homo sapiens
10 RPLP2 6181
Affinity Capture-MS Homo sapiens
11 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
12 LIN28A  
Affinity Capture-MS Homo sapiens
13 DAW1  
Affinity Capture-MS Homo sapiens
14 HIST1H2BB 3018
Cross-Linking-MS (XL-MS) Homo sapiens
15 SETD8  
Affinity Capture-MS Homo sapiens
16 RLIM 51132
Affinity Capture-MS Homo sapiens
17 HIST1H2BJ 8970
Cross-Linking-MS (XL-MS) Homo sapiens
18 S100A7 6278
Affinity Capture-MS Homo sapiens
19 HIST1H2BN 8341
Cross-Linking-MS (XL-MS) Homo sapiens
20 HIST1H1D 3007
Cross-Linking-MS (XL-MS) Homo sapiens
21 HIST3H2BB 128312
Cross-Linking-MS (XL-MS) Homo sapiens
22 CUL4B 8450
Affinity Capture-MS Homo sapiens
23 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
24 HMGN2 3151
Affinity Capture-MS Homo sapiens
25 S100A9 6280
Affinity Capture-MS Homo sapiens
26 HIST1H1B 3009
Cross-Linking-MS (XL-MS) Homo sapiens
27 BLM 641
Synthetic Growth Defect Homo sapiens
28 S100A8 6279
Affinity Capture-MS Homo sapiens
29 CAND1 55832
Affinity Capture-MS Homo sapiens
30 HIST2H2BF 440689
Cross-Linking-MS (XL-MS) Homo sapiens
31 HIST1H1A 3024
Cross-Linking-MS (XL-MS) Homo sapiens
32 LOC102724334 102724334
Cross-Linking-MS (XL-MS) Homo sapiens
33 HIST1H2BO 8348
Cross-Linking-MS (XL-MS) Homo sapiens
34 RPL35 11224
Affinity Capture-MS Homo sapiens
35 PABPC1 26986
Affinity Capture-MS Homo sapiens
36 MCM2 4171
Affinity Capture-MS Homo sapiens
37 RPL7A 6130
Affinity Capture-MS Homo sapiens
38 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
39 RPL35A 6165
Affinity Capture-MS Homo sapiens
40 COPS5 10987
Affinity Capture-MS Homo sapiens
41 RPL36 25873
Affinity Capture-MS Homo sapiens
42 PCGF1 84759
Affinity Capture-MS Homo sapiens
43 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
44 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
45 CUL4A 8451
Affinity Capture-MS Homo sapiens
46 HIST1H1T 3010
Cross-Linking-MS (XL-MS) Homo sapiens
47 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
48 H2AFY 9555
Cross-Linking-MS (XL-MS) Homo sapiens
49 NEDD8 4738
Affinity Capture-MS Homo sapiens
50 CUL5 8065
Affinity Capture-MS Homo sapiens
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