Gene description for SCRIB
Gene name scribbled planar cell polarity protein
Gene symbol SCRIB
Other names/aliases CRIB1
SCRB1
SCRIB1
Vartul
Species Homo sapiens
 Database cross references - SCRIB
ExoCarta ExoCarta_23513
Vesiclepedia VP_23513
Entrez Gene 23513
HGNC 30377
MIM 607733
UniProt Q14160  
 SCRIB identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for SCRIB
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IBA
    signaling adaptor activity GO:0035591 IDA
    cadherin binding GO:0045296 HDA
Biological Process
    establishment of T cell polarity GO:0001768 ISS
    neural tube closure GO:0001843 IMP
    positive regulation of receptor recycling GO:0001921 IMP
    cell population proliferation GO:0008283 IDA
    epithelial structure maintenance GO:0010669 ISS
    synaptic vesicle targeting GO:0016080 IEA
    cell migration GO:0016477 IBA
    cell migration GO:0016477 IMP
    cochlear nucleus development GO:0021747 IEA
    polarized epithelial cell differentiation GO:0030859 IMP
    positive regulation of type II interferon production GO:0032729 ISS
    establishment of apical/basal cell polarity GO:0035089 IMP
    post-anal tail morphogenesis GO:0036342 IEA
    wound healing GO:0042060 IEA
    positive regulation of apoptotic process GO:0043065 IMP
    receptor clustering GO:0043113 IBA
    astrocyte cell migration GO:0043615 IEA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IBA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 NAS
    negative regulation of mitotic cell cycle GO:0045930 IDA
    negative regulation of translational initiation GO:0045947 IMP
    negative regulation of activated T cell proliferation GO:0046007 ISS
    synaptic vesicle endocytosis GO:0048488 IEA
    positive chemotaxis GO:0050918 IMP
    auditory receptor cell stereocilium organization GO:0060088 IEA
    apoptotic process involved in morphogenesis GO:0060561 IMP
    mammary gland duct morphogenesis GO:0060603 ISS
    protein localization to adherens junction GO:0071896 IMP
    activation of GTPase activity GO:0090630 IMP
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion GO:0098609 IGI
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IBA
    neurotransmitter receptor transport postsynaptic membrane to endosome GO:0098968 IBA
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
Subcellular Localization
    immunological synapse GO:0001772 ISS
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    cell-cell junction GO:0005911 IDA
    adherens junction GO:0005912 IBA
    adherens junction GO:0005912 IDA
    adherens junction GO:0005912 NAS
    postsynaptic density GO:0014069 IBA
    basolateral plasma membrane GO:0016323 IBA
    lamellipodium GO:0030027 IEA
    cell junction GO:0030054 IDA
    cell leading edge GO:0031252 IDA
    Scrib-APC-beta-catenin complex GO:0034750 IDA
    myelin sheath abaxonal region GO:0035748 IEA
    presynaptic membrane GO:0042734 IDA
    cell-cell contact zone GO:0044291 IEA
    postsynaptic membrane GO:0045211 IBA
    postsynaptic membrane GO:0045211 IDA
    extracellular exosome GO:0070062 HDA
    glutamatergic synapse GO:0098978 IEA
    extrinsic component of postsynaptic density membrane GO:0099147 IEA
 Experiment description of studies that identified SCRIB in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 207
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 208
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
14
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 1275
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 1280
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 258
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 259
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 260
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
27
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
28
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
31
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
32
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
33
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SCRIB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL9 55958
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 C5AR2  
Two-hybrid Homo sapiens
5 PPP1CB 5500
Affinity Capture-MS Homo sapiens
6 PPP1R3A  
Affinity Capture-MS Homo sapiens
7 LGR4 55366
Affinity Capture-MS Homo sapiens
8 OCLN 100506658
Proximity Label-MS Homo sapiens
9 MARCKS 4082
Proximity Label-MS Homo sapiens
10 NOS1AP  
Affinity Capture-MS Homo sapiens
11 GJA1 2697
Proximity Label-MS Homo sapiens
12 ARHGEF26 26084
Affinity Capture-MS Homo sapiens
13 PARD3 56288
Proximity Label-MS Homo sapiens
14 TGS1  
Affinity Capture-MS Homo sapiens
15 EN1  
Affinity Capture-MS Homo sapiens
16 PAK2 5062
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 UBE3A 7337
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
18 C1orf226  
Affinity Capture-MS Homo sapiens
19 ZNF697  
Affinity Capture-MS Homo sapiens
20 TRAF7  
Affinity Capture-MS Homo sapiens
21 STX4 6810
Proximity Label-MS Homo sapiens
22 PPP1CC 5501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 LRRC27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 LAMTOR1 55004
Proximity Label-MS Homo sapiens
25 IRF2BP1 26145
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 RNPS1 10921
Affinity Capture-MS Homo sapiens
27 GIT2 9815
Affinity Capture-MS Homo sapiens
28 MLLT4 4301
Proximity Label-MS Homo sapiens
29 DYRK1A 1859
Affinity Capture-MS Homo sapiens
30 CLEC18A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ZNF638 27332
Affinity Capture-MS Homo sapiens
32 PIP4K2B 8396
Affinity Capture-MS Homo sapiens
33 OBSL1 23363
Affinity Capture-MS Homo sapiens
34 ZNF7  
Affinity Capture-MS Homo sapiens
35 SF3B3 23450
Affinity Capture-MS Homo sapiens
36 LRSAM1 90678
Affinity Capture-MS Homo sapiens
37 STX6 10228
Proximity Label-MS Homo sapiens
38 ITGA4 3676
Affinity Capture-MS Homo sapiens
39 ZNF219  
Affinity Capture-MS Homo sapiens
40 VCAM1 7412
Affinity Capture-MS Homo sapiens
41 CTNNA1 1495
Proximity Label-MS Homo sapiens
42 RAB35 11021
Proximity Label-MS Homo sapiens
43 PRKAG1 5571
Affinity Capture-MS Homo sapiens
44 EBAG9 9166
Proximity Label-MS Homo sapiens
45 ARHGEF15  
Affinity Capture-MS Homo sapiens
46 KCNA4  
Affinity Capture-MS Homo sapiens
47 KIF14 9928
Affinity Capture-MS Homo sapiens
48 PAK1 5058
Affinity Capture-MS Homo sapiens
49 TSHR 7253
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
50 PPP1CA 5499
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 GUCY1A2  
Affinity Capture-Western Homo sapiens
52 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
53 MAPK3 5595
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
54 MCC 4163
Affinity Capture-MS Homo sapiens
55 MAPK12  
Affinity Capture-Western Homo sapiens
56 PARK2  
Affinity Capture-MS Homo sapiens
57 CXADR 1525
Proximity Label-MS Homo sapiens
58 IRF2BPL 64207
Affinity Capture-MS Homo sapiens
59 ZNF592 9640
Affinity Capture-MS Homo sapiens
60 LYN 4067
Proximity Label-MS Homo sapiens
61 IL1R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 MCAM 4162
Proximity Label-MS Homo sapiens
63 RNF146  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
64 CPVL 54504
Affinity Capture-MS Homo sapiens
65 DNAJC5 80331
Proximity Label-MS Homo sapiens
66 MUSK  
Affinity Capture-Western Homo sapiens
67 CDH1 999
Proximity Label-MS Homo sapiens
68 CHMP4B 128866
Affinity Capture-MS Homo sapiens
69 RPA2 6118
Proximity Label-MS Homo sapiens
70 DIRAS3  
Proximity Label-MS Homo sapiens
71 NTRK1 4914
Affinity Capture-MS Homo sapiens
72 GIT1 28964
Affinity Capture-MS Homo sapiens
73 C17orf70  
Affinity Capture-MS Homo sapiens
74 PIGR 5284
Affinity Capture-MS Homo sapiens
75 ARRDC3 57561
Affinity Capture-MS Homo sapiens
76 ACTB 60
Proximity Label-MS Homo sapiens
77 ARHGEF6 9459
Affinity Capture-MS Homo sapiens
78 ZNF483  
Affinity Capture-MS Homo sapiens
79 APC  
Reconstituted Complex Homo sapiens
80 PRKAB1 5564
Affinity Capture-MS Homo sapiens
81 VPS11 55823
Affinity Capture-MS Homo sapiens
82 ZBTB44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 ANLN 54443
Affinity Capture-MS Homo sapiens
84 PHLPP1  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 FOXH1  
Affinity Capture-MS Homo sapiens
86 KIF1B 23095
Affinity Capture-MS Homo sapiens
87 OGT 8473
Reconstituted Complex Homo sapiens
88 ZNF316  
Affinity Capture-MS Homo sapiens
89 SHKBP1  
Affinity Capture-MS Homo sapiens
90 CUL3 8452
Affinity Capture-MS Homo sapiens
91 ZNF768 79724
Affinity Capture-MS Homo sapiens
92 CLK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 RAB5A 5868
Proximity Label-MS Homo sapiens
94 ECT2 1894
Affinity Capture-MS Homo sapiens
95 SUGT1 10910
Affinity Capture-MS Homo sapiens
96 LMO7 4008
Affinity Capture-MS Homo sapiens
97 EPB41L4A 64097
Proximity Label-MS Homo sapiens
98 CTNNB1 1499
Proximity Label-MS Homo sapiens
99 MEX3A  
Affinity Capture-RNA Homo sapiens
100 SETD2  
Affinity Capture-MS Homo sapiens
101 FOXG1  
Affinity Capture-MS Homo sapiens
102 CNPY3 10695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 DCUN1D2 55208
Affinity Capture-MS Homo sapiens
104 ARHGEF16 27237
Affinity Capture-MS Homo sapiens
105 FOXL1  
Affinity Capture-MS Homo sapiens
106 SLTM 79811
Affinity Capture-MS Homo sapiens
107 FN1 2335
Affinity Capture-MS Homo sapiens
108 NXF1 10482
Affinity Capture-RNA Homo sapiens
109 PPHLN1  
Affinity Capture-MS Homo sapiens
110 ZNF777  
Affinity Capture-MS Homo sapiens
111 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
112 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
113 TJP2 9414
Affinity Capture-MS Homo sapiens
114 Nup107  
Affinity Capture-MS Mus musculus
115 RPA3 6119
Proximity Label-MS Homo sapiens
116 LRIG1 26018
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 CEP170B  
Affinity Capture-MS Homo sapiens
118 PKP4 8502
Affinity Capture-Western Homo sapiens
119 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
120 PARP1 142
Proximity Label-MS Homo sapiens
121 RHOB 388
Proximity Label-MS Homo sapiens
122 PATZ1  
Affinity Capture-MS Homo sapiens
123 KRAS 3845
Proximity Label-MS Homo sapiens
124 P4HA3 283208
Affinity Capture-MS Homo sapiens
125 PRKAB2 5565
Affinity Capture-MS Homo sapiens
126 LRRC40 55631
Affinity Capture-MS Homo sapiens
127 Ppp1cb 19046
Affinity Capture-MS Mus musculus
128 FOXK1 221937
Affinity Capture-MS Homo sapiens
129 FLOT1 10211
Proximity Label-MS Homo sapiens
130 ZNF263  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 CCDC8  
Affinity Capture-MS Homo sapiens
132 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
133 PRKAA1 5562
Affinity Capture-MS Homo sapiens
134 EXOSC10 5394
Two-hybrid Homo sapiens
135 RNF43  
Proximity Label-MS Homo sapiens
136 KLHL13 90293
Affinity Capture-MS Homo sapiens
137 SSTR3  
Affinity Capture-MS Homo sapiens
138 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
139 SIRT6  
Affinity Capture-MS Homo sapiens
140 LRRC6  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 PDIA4 9601
Cross-Linking-MS (XL-MS) Homo sapiens
142 FOXB1  
Affinity Capture-MS Homo sapiens
143 EZR 7430
Proximity Label-MS Homo sapiens
144 ZNF446  
Affinity Capture-MS Homo sapiens
145 LCK 3932
Proximity Label-MS Homo sapiens
146 DMWD  
Affinity Capture-MS Homo sapiens
147 PRKAG2 51422
Affinity Capture-MS Homo sapiens
148 Shoc2  
Affinity Capture-MS Mus musculus
149 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
150 SHMT2 6472
Affinity Capture-RNA Homo sapiens
151 C11orf52 91894
Proximity Label-MS Homo sapiens
152 MKI67  
Affinity Capture-MS Homo sapiens
153 NEK1  
Affinity Capture-MS Homo sapiens
154 CCT7 10574
Cross-Linking-MS (XL-MS) Homo sapiens
155 ZNF317 57693
Affinity Capture-MS Homo sapiens
156 RBM23  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
157 MOV10 4343
Affinity Capture-RNA Homo sapiens
158 C16orf72 29035
Affinity Capture-MS Homo sapiens
159 PRKCA 5578
Biochemical Activity Homo sapiens
160 BRD1 23774
Affinity Capture-MS Homo sapiens
161 EPHA2 1969
Proximity Label-MS Homo sapiens
162 TXNIP 10628
Affinity Capture-MS Homo sapiens
163 Sptlc2  
Affinity Capture-MS Mus musculus
164 C9orf72  
Affinity Capture-MS Homo sapiens
165 VIM 7431
Affinity Capture-MS Homo sapiens
166 IRF2BP2  
Affinity Capture-MS Homo sapiens
167 FOXE1  
Affinity Capture-MS Homo sapiens
168 ZNF449  
Affinity Capture-MS Homo sapiens
169 CAV1 857
Proximity Label-MS Homo sapiens
170 ZKSCAN8  
Affinity Capture-MS Homo sapiens
171 ZNF628  
Affinity Capture-MS Homo sapiens
172 EP300 2033
Affinity Capture-MS Homo sapiens
173 CDC5L 988
Affinity Capture-MS Homo sapiens
174 LRRC1 55227
Affinity Capture-MS Homo sapiens
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