Gene description for DCUN1D2
Gene name DCN1, defective in cullin neddylation 1, domain containing 2
Gene symbol DCUN1D2
Other names/aliases C13orf17
Species Homo sapiens
 Database cross references - DCUN1D2
ExoCarta ExoCarta_55208
Vesiclepedia VP_55208
Entrez Gene 55208
HGNC 20328
UniProt Q6PH85  
 DCUN1D2 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DCUN1D2
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin conjugating enzyme binding GO:0031624 IBA
    ubiquitin-like protein binding GO:0032182 IBA
    cullin family protein binding GO:0097602 IBA
    cullin family protein binding GO:0097602 IMP
Biological Process
    protein neddylation GO:0045116 IBA
    regulation of protein neddylation GO:2000434 IMP
    positive regulation of protein neddylation GO:2000436 IMP
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IBA
    nucleus GO:0005634 IMP
    cytoplasm GO:0005737 IMP
 Experiment description of studies that identified DCUN1D2 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DCUN1D2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2F  
Reconstituted Complex Homo sapiens
2 KLHL18  
Affinity Capture-MS Homo sapiens
3 CUL1 8454
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 SPRTN  
Affinity Capture-MS Homo sapiens
5 MAP3K1 4214
Biochemical Activity Homo sapiens
6 USP25  
Affinity Capture-MS Homo sapiens
7 INPPL1 3636
Affinity Capture-MS Homo sapiens
8 UBE2M 9040
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 CUL5 8065
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 CUL3 8452
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
11 RBX1 9978
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 ZC3HC1  
Affinity Capture-MS Homo sapiens
13 CUL4B 8450
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GAP43  
Affinity Capture-MS Homo sapiens
15 FEM1B 10116
Affinity Capture-MS Homo sapiens
16 KBTBD7  
Affinity Capture-MS Homo sapiens
17 APP 351
Reconstituted Complex Homo sapiens
18 RNF7  
Affinity Capture-MS Homo sapiens
19 CAND1 55832
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 KLHL42  
Affinity Capture-MS Homo sapiens
21 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
22 CARM1 10498
Affinity Capture-MS Homo sapiens
23 KLHL24  
Affinity Capture-MS Homo sapiens
24 SCCPDH 51097
Affinity Capture-MS Homo sapiens
25 TCEB2 6923
Affinity Capture-MS Homo sapiens
26 SCRIB 23513
Affinity Capture-MS Homo sapiens
27 CUL2 8453
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
28 KCTD3  
Affinity Capture-MS Homo sapiens
29 CUL4A 8451
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which DCUN1D2 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Neddylation TAS Reactome
Post-translational protein modification TAS Reactome





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