Experiment description of studies that identified SLTM in sEVs

1 Experiment ID 426 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 427 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

9 Experiment ID 434 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 435 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

11 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

12 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for SLTM

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HIST1H2BC 8347 Cross-Linking-MS (XL-MS) Homo sapiens 2 SENP3 26168 Affinity Capture-MS Homo sapiens 3 Fus   Affinity Capture-MS Mus musculus 4 GGPS1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 MCC 4163 Two-hybrid Homo sapiens 6 FGFBP1 9982 Affinity Capture-MS Homo sapiens 7 ANAPC15   Affinity Capture-MS Homo sapiens 8 DHX8 1659 Proximity Label-MS Homo sapiens 9 MAP4 4134 Affinity Capture-MS Homo sapiens 10 CALM1 801 Reconstituted Complex Homo sapiens 11 SOX2   Affinity Capture-MS Homo sapiens 12 ANKRD50 57182 Affinity Capture-MS Homo sapiens 13 CHCHD4   Affinity Capture-MS Homo sapiens 14 DDX23 9416 Proximity Label-MS Homo sapiens 15 C2orf73   Affinity Capture-MS Homo sapiens 16 SNRNP70 6625 Affinity Capture-MS Homo sapiens 17 PRPF38B 55119 Affinity Capture-MS Homo sapiens 18 PRC1 9055 Affinity Capture-MS Homo sapiens 19 SCRIB 23513 Affinity Capture-MS Homo sapiens 20 OBSL1 23363 Affinity Capture-MS Homo sapiens 21 DDX58 23586 Affinity Capture-RNA Homo sapiens 22 PEA15 8682 Affinity Capture-MS Homo sapiens 23 EXOSC4 54512 Affinity Capture-MS Homo sapiens 24 EED   Affinity Capture-MS Homo sapiens 25 SDHAP2   Affinity Capture-MS Homo sapiens 26 ATG16L1 55054 Affinity Capture-MS Homo sapiens 27 MECP2 4204 Affinity Capture-MS Homo sapiens 28 MYCN   Affinity Capture-MS Homo sapiens 29 TACO1   Affinity Capture-MS Homo sapiens 30 CLK3   Affinity Capture-MS Homo sapiens 31 IFNA14   Affinity Capture-MS Homo sapiens 32 LTBR 4055 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 CHMP4C 92421 Affinity Capture-MS Homo sapiens 34 TMEM14B   Affinity Capture-MS Homo sapiens 35 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 36 DDX21 9188 Affinity Capture-MS Homo sapiens 37 RBM4B   Affinity Capture-MS Homo sapiens 38 Taf15   Affinity Capture-MS Mus musculus 39 IFI27L1   Affinity Capture-MS Homo sapiens 40 CEP19   Affinity Capture-MS Homo sapiens 41 PTP4A3   Affinity Capture-MS Homo sapiens 42 AIM2   Affinity Capture-MS Homo sapiens 43 IFI16 3428 Affinity Capture-MS Homo sapiens 44 RPA4   Proximity Label-MS Homo sapiens 45 IKZF5 64376 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 ARL13B 200894 Affinity Capture-MS Homo sapiens 47 CCNT1   Affinity Capture-MS Homo sapiens 48 ID2   Affinity Capture-MS Homo sapiens 49 C1orf189   Affinity Capture-MS Homo sapiens 50 POLR2B 5431 Co-fractionation Homo sapiens 51 ANLN 54443 Affinity Capture-MS Homo sapiens 52 TPX2   Affinity Capture-MS Homo sapiens 53 SFPQ 6421 Co-fractionation Homo sapiens 54 FANCD2   Affinity Capture-MS Homo sapiens 55 BTNL9   Affinity Capture-MS Homo sapiens 56 LMNB1 4001 Affinity Capture-MS Homo sapiens 57 PSENEN   Affinity Capture-MS Homo sapiens 58 SIRT7   Affinity Capture-MS Homo sapiens 59 ERLEC1 27248 Affinity Capture-MS Homo sapiens 60 HNRNPA1 3178 Proximity Label-MS Homo sapiens 61 GHITM 27069 Affinity Capture-MS Homo sapiens 62 SUZ12   Affinity Capture-MS Homo sapiens 63 CUL9   Affinity Capture-MS Homo sapiens 64 MYC   Affinity Capture-MS Homo sapiens 65 RPA3 6119 Proximity Label-MS Homo sapiens 66 ZC3H18   Affinity Capture-MS Homo sapiens 67 PABPC5 140886 Affinity Capture-MS Homo sapiens 68 TOP1 7150 Affinity Capture-MS Homo sapiens 69 KAT5   Affinity Capture-MS Homo sapiens 70 MATR3 9782 Affinity Capture-MS Homo sapiens 71 DPM2 8818 Affinity Capture-MS Homo sapiens 72 GPBP1   Cross-Linking-MS (XL-MS) Homo sapiens 73 BMI1   Affinity Capture-MS Homo sapiens 74 ZC3HAV1 56829 Co-fractionation Homo sapiens 75 TMSB4Y   Affinity Capture-MS Homo sapiens 76 MALL   Affinity Capture-MS Homo sapiens 77 FGL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 78 FDCSP   Affinity Capture-MS Homo sapiens 79 MAPKAPK2 9261 Affinity Capture-MS Homo sapiens 80 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 81 FASN 2194 Positive Genetic Homo sapiens 82 TERF2   Affinity Capture-MS Homo sapiens 83 PLEC 5339 Co-fractionation Homo sapiens 84 HIST1H2BK 85236 Cross-Linking-MS (XL-MS) Homo sapiens 85 DNAJC19 131118 Co-fractionation Homo sapiens 86 CPSF6 11052 Affinity Capture-MS Homo sapiens 87 FANCC 2176 Affinity Capture-MS Homo sapiens 88 PCMT1 5110 Cross-Linking-MS (XL-MS) Homo sapiens 89 APOBEC3D   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 90 PPM1J   Affinity Capture-MS Homo sapiens 91 SAA1 6288 Affinity Capture-MS Homo sapiens 92 CCDC70   Affinity Capture-MS Homo sapiens 93 SNRPA 6626 Affinity Capture-MS Homo sapiens 94 KNOP1 400506 Affinity Capture-MS Homo sapiens 95 IFI6   Affinity Capture-MS Homo sapiens 96 EZH1   Affinity Capture-MS Homo sapiens 97 PIP4K2A 5305 Affinity Capture-MS Homo sapiens 98 DHX40   Proximity Label-MS Homo sapiens 99 C20orf197   Affinity Capture-MS Homo sapiens 100 Srsf1 110809 Affinity Capture-MS Mus musculus 101 E2F4   Affinity Capture-MS Homo sapiens 102 LRPAP1 4043 Affinity Capture-MS Homo sapiens 103 SPCS1 28972 Affinity Capture-MS Homo sapiens 104 SSSCA1 10534 Affinity Capture-MS Homo sapiens 105 RPS6 6194 Affinity Capture-MS Homo sapiens 106 GAGE5   Affinity Capture-MS Homo sapiens 107 EP300 2033 Affinity Capture-MS Homo sapiens 108 SSRP1 6749 Affinity Capture-MS Homo sapiens 109 UQCR11   Affinity Capture-MS Homo sapiens 110 ZNF677   Affinity Capture-MS Homo sapiens View the network

Pathways in which SLTM is involved

No pathways found