Gene description for TXNIP
Gene name thioredoxin interacting protein
Gene symbol TXNIP
Other names/aliases EST01027
HHCPA78
THIF
VDUP1
Species Homo sapiens
 Database cross references - TXNIP
ExoCarta ExoCarta_10628
Vesiclepedia VP_10628
Entrez Gene 10628
HGNC 16952
MIM 606599
UniProt Q9H3M7  
 TXNIP identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TXNIP
Molecular Function
    enzyme inhibitor activity GO:0004857 IDA
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    protein import into nucleus GO:0006606 IEA
    inflammatory response GO:0006954 TAS
    response to oxidative stress GO:0006979 IDA
    response to xenobiotic stimulus GO:0009410 IEA
    response to mechanical stimulus GO:0009612 IEA
    response to glucose GO:0009749 IEA
    protein transport GO:0015031 IBA
    keratinocyte differentiation GO:0030216 IDA
    response to estradiol GO:0032355 IEA
    response to progesterone GO:0032570 IEA
    regulation of cell population proliferation GO:0042127 IEA
    response to hydrogen peroxide GO:0042542 IEA
    positive regulation of apoptotic process GO:0043065 IEA
    platelet-derived growth factor receptor signaling pathway GO:0048008 IEA
    response to calcium ion GO:0051592 IEA
    negative regulation of cell division GO:0051782 IDA
    cellular response to tumor cell GO:0071228 IDA
    cellular response to oxidised low-density lipoprotein particle stimulus GO:0140052 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified TXNIP in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TXNIP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIF16B 55614
Affinity Capture-MS Homo sapiens
2 PRKAA1 5562
Biochemical Activity Homo sapiens
3 GATAD2B 57459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 HDAC2 3066
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 MTR 4548
Affinity Capture-MS Homo sapiens
6 WWP1 11059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
7 VPRBP 9730
Affinity Capture-MS Homo sapiens
8 SIVA1  
Two-hybrid Homo sapiens
9 SLC2A1 6513
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
10 VHL  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 PIK3CA 5290
Affinity Capture-MS Homo sapiens
12 DDIT4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
13 PI4KA 5297
Affinity Capture-MS Homo sapiens
14 CWF19L1 55280
Affinity Capture-MS Homo sapiens
15 INTS9 55756
Two-hybrid Homo sapiens
16 ZCCHC2 54877
Affinity Capture-MS Homo sapiens
17 KBTBD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 S100A4 6275
Affinity Capture-MS Homo sapiens
19 PJA1  
Affinity Capture-MS Homo sapiens
20 WDR11 55717
Affinity Capture-MS Homo sapiens
21 PMPCA 23203
Affinity Capture-MS Homo sapiens
22 LRMP  
Affinity Capture-MS Homo sapiens
23 SMARCA1 6594
Affinity Capture-MS Homo sapiens
24 COPS5 10987
Affinity Capture-Western Homo sapiens
25 FBXW7  
Affinity Capture-MS Homo sapiens
26 SCRIB 23513
Affinity Capture-MS Homo sapiens
27 RUVBL1 8607
Affinity Capture-MS Homo sapiens
28 KDR  
Affinity Capture-Western Homo sapiens
29 FLG 2312
Affinity Capture-MS Homo sapiens
30 HERC2 8924
Affinity Capture-MS Homo sapiens
31 PFKFB3 5209
Affinity Capture-MS Homo sapiens
32 PMPCB 9512
Affinity Capture-MS Homo sapiens
33 EEF1A1 1915
Two-hybrid Homo sapiens
34 NPHP3 27031
Affinity Capture-MS Homo sapiens
35 HSPA8 3312
Affinity Capture-MS Homo sapiens
36 CCT2 10576
Affinity Capture-MS Homo sapiens
37 MAPK1 5594
Biochemical Activity Homo sapiens
38 EPB41L3 23136
Affinity Capture-MS Homo sapiens
39 XPO1 7514
Affinity Capture-Western Homo sapiens
40 RBBP7 5931
Affinity Capture-MS Homo sapiens
41 UXT 8409
Two-hybrid Homo sapiens
42 SCEL 8796
Two-hybrid Homo sapiens
43 PSMD4 5710
Affinity Capture-MS Homo sapiens
44 ZBTB16  
Affinity Capture-Western Homo sapiens
45 MAP1B 4131
Affinity Capture-MS Homo sapiens
46 HSP90B1 7184
Affinity Capture-MS Homo sapiens
47 TUBA1A 7846
Affinity Capture-MS Homo sapiens
48 BAG6 7917
Affinity Capture-MS Homo sapiens
49 ZSWIM8 23053
Affinity Capture-MS Homo sapiens
50 PJA2  
Affinity Capture-MS Homo sapiens
51 RGMA  
Affinity Capture-MS Homo sapiens
52 MAGED4  
Affinity Capture-MS Homo sapiens
53 TUBA1C 84790
Affinity Capture-MS Homo sapiens
54 PAPD7  
Affinity Capture-MS Homo sapiens
55 ZBTB32  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
56 USP5 8078
Affinity Capture-MS Homo sapiens
57 RAD18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 S100B 6285
Affinity Capture-MS Homo sapiens
59 GTF3C1  
Affinity Capture-MS Homo sapiens
60 CRY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 MBD3 53615
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 ITCH 83737
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
63 PRPF31 26121
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
64 RBM10  
Affinity Capture-MS Homo sapiens
65 TXN2 25828
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
66 NEURL4  
Affinity Capture-MS Homo sapiens
67 CAD 790
Affinity Capture-MS Homo sapiens
68 PTPN11 5781
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
69 GTF3C3 9330
Affinity Capture-MS Homo sapiens
70 TUBB 203068
Affinity Capture-MS Homo sapiens
71 MYCBP2 23077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 ECD  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
73 RAB5A 5868
Affinity Capture-Western Homo sapiens
74 DOCK7 85440
Affinity Capture-MS Homo sapiens
75 RLIM 51132
Affinity Capture-MS Homo sapiens
76 PRKAA2 5563
Affinity Capture-Western Homo sapiens
77 HRNR 388697
Affinity Capture-MS Homo sapiens
78 TUBB4B 10383
Affinity Capture-MS Homo sapiens
79 EGFR 1956
Affinity Capture-Western Homo sapiens
80 DCAF16 54876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 POLR2A 5430
Affinity Capture-MS Homo sapiens
83 IL12RB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 IDI1 3422
Two-hybrid Homo sapiens
85 SCARA5 286133
Affinity Capture-MS Homo sapiens
86 HSPA1A 3303
Affinity Capture-MS Homo sapiens
87 HSPA4 3308
Affinity Capture-Western Homo sapiens
88 PSMB5 5693
Affinity Capture-MS Homo sapiens
89 CDKN1B 1027
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
90 Cltc 67300
Affinity Capture-Western Mus musculus
91 PER1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 NEDD4L 23327
Affinity Capture-MS Homo sapiens
93 HUWE1 10075
Affinity Capture-MS Homo sapiens
94 HIF1A 3091
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
95 RELA 5970
Affinity Capture-Western Homo sapiens
96 FHL1 2273
Two-hybrid Homo sapiens
97 HSPB2  
Two-hybrid Homo sapiens
98 TP53 7157
Affinity Capture-Western Homo sapiens
99 RUVBL2 10856
Two-hybrid Homo sapiens
100 STK38 11329
Affinity Capture-MS Homo sapiens
101 NLGN3  
Two-hybrid Homo sapiens
102 UBA52 7311
Affinity Capture-MS Homo sapiens
103 TTBK1  
Affinity Capture-MS Homo sapiens
104 TUBB3 10381
Affinity Capture-MS Homo sapiens
105 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
106 MTA1 9112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 HDAC1 3065
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
108 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
109 GET4 51608
Affinity Capture-MS Homo sapiens
110 TUBB6 84617
Affinity Capture-MS Homo sapiens
111 UBE3C 9690
Affinity Capture-MS Homo sapiens
112 DCD 117159
Affinity Capture-MS Homo sapiens
113 WWP2 11060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 TXN 7295
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
115 HDAC3 8841
Reconstituted Complex Homo sapiens
116 GAPDH 2597
Two-hybrid Homo sapiens
117 EIF4B 1975
Affinity Capture-MS Homo sapiens
118 Ap2a2 11772
Affinity Capture-Western Mus musculus
119 LUC7L3 51747
Two-hybrid Homo sapiens
120 FLNA 2316
Affinity Capture-MS Homo sapiens
121 CBX8 57332
Two-hybrid Homo sapiens
122 SSSCA1 10534
Affinity Capture-MS Homo sapiens
123 TXNIP 10628
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
124 SMURF2 64750
Two-hybrid Homo sapiens
125 PSMD1 5707
Affinity Capture-MS Homo sapiens
126 CHAF1B  
Affinity Capture-MS Homo sapiens
127 CCT8L2  
Affinity Capture-MS Homo sapiens
128 PKN3 29941
Affinity Capture-MS Homo sapiens
129 WDHD1  
Affinity Capture-MS Homo sapiens
130 FAM91A1 157769
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 NLRP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
132 KDM5C  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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