Gene description for FLG
Gene name filaggrin
Gene symbol FLG
Other names/aliases ATOD2
Species Homo sapiens
 Database cross references - FLG
ExoCarta ExoCarta_2312
Vesiclepedia VP_2312
Entrez Gene 2312
HGNC 3748
MIM 135940
UniProt P20930  
 FLG identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for FLG
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    structural constituent of skin epidermis GO:0030280 IDA
    transition metal ion binding GO:0046914 IEA
Biological Process
    peptide cross-linking GO:0018149 IDA
    keratinocyte differentiation GO:0030216 TAS
    establishment of skin barrier GO:0061436 IBA
    establishment of skin barrier GO:0061436 IEP
Subcellular Localization
    cornified envelope GO:0001533 IBA
    cornified envelope GO:0001533 IDA
    nucleus GO:0005634 HDA
    cytosol GO:0005829 TAS
    keratohyalin granule GO:0036457 IBA
    keratohyalin granule GO:0036457 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified FLG in sEVs
1
Experiment ID 494
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FLG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APTX  
Affinity Capture-MS Homo sapiens
2 PRSS16 10279
Affinity Capture-MS Homo sapiens
3 KRT18 3875
Cross-Linking-MS (XL-MS) Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 FBXW7  
Affinity Capture-MS Homo sapiens
6 GALNT9  
Affinity Capture-MS Homo sapiens
7 MRM1  
Affinity Capture-MS Homo sapiens
8 DTL  
Affinity Capture-MS Homo sapiens
9 STK16 8576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BLMH 642
Affinity Capture-MS Homo sapiens
11 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
12 INPP5A 3632
Affinity Capture-MS Homo sapiens
13 SLC25A6 293
Affinity Capture-MS Homo sapiens
14 C18orf21  
Affinity Capture-MS Homo sapiens
15 MAK  
Affinity Capture-MS Homo sapiens
16 GMCL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 METTL14  
Affinity Capture-MS Homo sapiens
18 CCNC  
Affinity Capture-MS Homo sapiens
19 ATG5 9474
Proximity Label-MS Homo sapiens
20 FCF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ANXA2 302
Affinity Capture-MS Homo sapiens
22 USP1 7398
Affinity Capture-MS Homo sapiens
23 SH2D3A  
Affinity Capture-MS Homo sapiens
24 TBC1D22B  
Affinity Capture-MS Homo sapiens
25 MCL1 4170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SLC35F2 54733
Affinity Capture-MS Homo sapiens
27 TUBG1 7283
Affinity Capture-MS Homo sapiens
28 UCP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ZSCAN12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ERCC3  
Affinity Capture-MS Homo sapiens
31 C1orf35  
Affinity Capture-MS Homo sapiens
32 ERP27  
Affinity Capture-MS Homo sapiens
33 NPHP1  
Affinity Capture-MS Homo sapiens
34 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
35 ZC3HC1  
Affinity Capture-MS Homo sapiens
36 PTDSS1 9791
Affinity Capture-MS Homo sapiens
37 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
38 LRRC10  
Affinity Capture-MS Homo sapiens
39 FTH1 2495
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CASP14 23581
Affinity Capture-MS Homo sapiens
41 PKP1 5317
Affinity Capture-MS Homo sapiens
42 FNDC5  
Affinity Capture-MS Homo sapiens
43 PI4KA 5297
Affinity Capture-MS Homo sapiens
44 C21orf59 56683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PSMD14 10213
Affinity Capture-MS Homo sapiens
46 KLF5  
Affinity Capture-MS Homo sapiens
47 E2F6 1876
Affinity Capture-MS Homo sapiens
48 DPPA4  
Affinity Capture-MS Homo sapiens
49 FRMD1  
Affinity Capture-MS Homo sapiens
50 KIR3DS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PI4KAP1  
Affinity Capture-MS Homo sapiens
52 EZH2  
Affinity Capture-MS Homo sapiens
53 TGM3 7053
Affinity Capture-MS Homo sapiens
54 TMEM79  
Affinity Capture-MS Homo sapiens
55 TRIM21 6737
Affinity Capture-MS Homo sapiens
56 KIF23 9493
Affinity Capture-MS Homo sapiens
57 PINK1  
Affinity Capture-MS Homo sapiens
58 GPR18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 GOT1 2805
Affinity Capture-MS Homo sapiens
60 SARAF  
Affinity Capture-MS Homo sapiens
61 DSG1 1828
Affinity Capture-MS Homo sapiens
62 STK11 6794
Affinity Capture-MS Homo sapiens
63 ACAD9 28976
Affinity Capture-MS Homo sapiens
64 TXNIP 10628
Affinity Capture-MS Homo sapiens
65 ALDH3A1 218
Affinity Capture-MS Homo sapiens
66 ZDHHC19  
Affinity Capture-MS Homo sapiens
67 B3GNT2 10678
Affinity Capture-MS Homo sapiens
68 OR2A4 79541
Affinity Capture-MS Homo sapiens
69 TNFRSF19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 OR13C3  
Affinity Capture-MS Homo sapiens
71 CUL2 8453
Affinity Capture-MS Homo sapiens
72 DDX3X 1654
Affinity Capture-MS Homo sapiens
73 AQP12B  
Affinity Capture-MS Homo sapiens
74 CCR1  
Affinity Capture-MS Homo sapiens
75 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
76 SCN3B  
Affinity Capture-MS Homo sapiens
77 FIBIN  
Affinity Capture-MS Homo sapiens
78 STX17 55014
Affinity Capture-MS Homo sapiens
79 P2RX6  
Affinity Capture-MS Homo sapiens
80 RIPPLY3  
Affinity Capture-MS Homo sapiens
81 CYLD  
Affinity Capture-MS Homo sapiens
82 MECP2 4204
Affinity Capture-MS Homo sapiens
83 MLLT6  
Affinity Capture-MS Homo sapiens
84 UCHL5 51377
Reconstituted Complex Homo sapiens
85 FLG2 388698
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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