Experiment description of studies that identified CPVL in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for CPVL

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Affinity Capture-MS Homo sapiens 2 HDAC2 3066 Affinity Capture-MS Homo sapiens 3 DCTN6 10671 Affinity Capture-MS Homo sapiens 4 KLF9 687 Affinity Capture-MS Homo sapiens 5 RPA2 6118 Proximity Label-MS Homo sapiens 6 ARHGEF9   Affinity Capture-MS Homo sapiens 7 C9orf78 51759 Affinity Capture-MS Homo sapiens 8 SEPT2 4735 Affinity Capture-MS Homo sapiens 9 ELF4 2000 Proximity Label-MS Homo sapiens 10 SEPT9 10801 Affinity Capture-MS Homo sapiens 11 CAPZB 832 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 QSOX2 169714 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 WDR76   Affinity Capture-MS Homo sapiens 14 TSC22D4 81628 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 KLF8   Proximity Label-MS Homo sapiens 16 FAM212A   Affinity Capture-MS Homo sapiens 17 COPB2 9276 Affinity Capture-MS Homo sapiens 18 PPP1R18 170954 Affinity Capture-MS Homo sapiens 19 SCRIB 23513 Affinity Capture-MS Homo sapiens 20 ACTR1A 10121 Affinity Capture-MS Homo sapiens 21 ATAD5   Affinity Capture-MS Homo sapiens 22 MAD2L2 10459 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 NT5C3A 51251 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 ABCE1 6059 Affinity Capture-MS Homo sapiens 25 SEPT8 23176 Affinity Capture-MS Homo sapiens 26 SARNP 84324 Affinity Capture-MS Homo sapiens 27 CEP57   Affinity Capture-MS Homo sapiens 28 CLTC 1213 Affinity Capture-MS Homo sapiens 29 BTRC 8945 Affinity Capture-MS Homo sapiens 30 SOX5 6660 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 KLF16   Proximity Label-MS Homo sapiens 32 DNAJC14   Affinity Capture-MS Homo sapiens 33 TLX3   Proximity Label-MS Homo sapiens 34 TMEM177   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 CDKN1A   Affinity Capture-MS Homo sapiens 36 PARK2   Affinity Capture-MS Homo sapiens 37 DCTN5 84516 Affinity Capture-MS Homo sapiens 38 Sec24c   Affinity Capture-MS Mus musculus 39 FAM162A 26355 Affinity Capture-MS Homo sapiens 40 PTPRE 5791 Affinity Capture-MS Homo sapiens 41 YAP1 10413 Affinity Capture-MS Homo sapiens 42 SAR1B 51128 Affinity Capture-MS Homo sapiens 43 LHX3   Proximity Label-MS Homo sapiens 44 NRBF2   Affinity Capture-MS Homo sapiens 45 PAX8   Proximity Label-MS Homo sapiens 46 ASB10   Affinity Capture-MS Homo sapiens 47 LETMD1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 48 LATS2 26524 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 49 MICU1 10367 Affinity Capture-MS Homo sapiens 50 KLF12   Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 51 SEC61B 10952 Proximity Label-MS Homo sapiens 52 CTNNBIP1 56998 Affinity Capture-MS Homo sapiens 53 ERLEC1 27248 Affinity Capture-MS Homo sapiens 54 CANX 821 Affinity Capture-MS Homo sapiens 55 AMBRA1   Affinity Capture-MS Homo sapiens 56 SOX6   Proximity Label-MS Homo sapiens 57 PLRG1 5356 Affinity Capture-MS Homo sapiens 58 CUL3 8452 Affinity Capture-MS Homo sapiens 59 NR3C1 2908 Proximity Label-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 60 TUBB4B 10383 Affinity Capture-MS Homo sapiens 61 EGFR 1956 Affinity Capture-MS Homo sapiens 62 HSD17B10 3028 Affinity Capture-MS Homo sapiens 63 FBXO6 26270 Affinity Capture-MS Homo sapiens 64 SEPT11 55752 Affinity Capture-MS Homo sapiens 65 NAT14 57106 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 66 ABHD12 26090 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 67 OCIAD1 54940 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 68 SRF   Affinity Capture-MS Homo sapiens 69 C22orf23   Affinity Capture-MS Homo sapiens 70 SS18L2   Affinity Capture-MS Homo sapiens 71 RPA3 6119 Proximity Label-MS Homo sapiens 72 NUCB1 4924 Affinity Capture-MS Homo sapiens 73 RASSF10   Affinity Capture-MS Homo sapiens 74 SEPT7 989 Affinity Capture-MS Homo sapiens 75 HUWE1 10075 Affinity Capture-MS Homo sapiens 76 MANEA 79694 Affinity Capture-MS Homo sapiens 77 NEXN 91624 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 78 TP53BP2   Affinity Capture-MS Homo sapiens 79 CDC20 991 Affinity Capture-MS Homo sapiens 80 TBC1D7   Affinity Capture-MS Homo sapiens 81 KLF3   Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 82 CAPZA2 830 Affinity Capture-MS Homo sapiens 83 Arhgap30   Affinity Capture-MS Mus musculus 84 ACTR1B 10120 Affinity Capture-MS Homo sapiens 85 DCTN2 10540 Affinity Capture-MS Homo sapiens 86 TSC1 7248 Affinity Capture-MS Homo sapiens 87 PAX9   Proximity Label-MS Homo sapiens 88 PPP1R13B   Affinity Capture-MS Homo sapiens 89 GLI2   Affinity Capture-MS Homo sapiens 90 C16orf72 29035 Affinity Capture-MS Homo sapiens 91 PHACTR3   Affinity Capture-MS Homo sapiens 92 CYB5R3 1727 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 93 ERG   Proximity Label-MS Homo sapiens 94 PTPN18 26469 Affinity Capture-MS Homo sapiens 95 ZMAT4   Affinity Capture-MS Homo sapiens 96 SP7   Proximity Label-MS Homo sapiens View the network

Pathways in which CPVL is involved

No pathways found