Gene description for TAX1BP3
Gene name Tax1 (human T-cell leukemia virus type I) binding protein 3
Gene symbol TAX1BP3
Other names/aliases TIP-1
Species Homo sapiens
 Database cross references - TAX1BP3
ExoCarta ExoCarta_30851
Vesiclepedia VP_30851
Entrez Gene 30851
HGNC 30684
UniProt O14907  
 TAX1BP3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
 Gene ontology annotations for TAX1BP3
Molecular Function
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IEA
Biological Process
    Rho protein signal transduction GO:0007266 IDA
    negative regulation of cell population proliferation GO:0008285 IEA
    Wnt signaling pathway GO:0016055 IEA
    negative regulation of Wnt signaling pathway GO:0030178 ISS
    activation of GTPase activity GO:0090630 IDA
    negative regulation of protein localization to cell surface GO:2000009 IDA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    actin cytoskeleton GO:0015629 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified TAX1BP3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
10
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for TAX1BP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATA1  
Two-hybrid Homo sapiens
2 TOMM40 10452
Co-fractionation Homo sapiens
3 CTNNA2 1496
Affinity Capture-MS Homo sapiens
4 FASN 2194
Positive Genetic Homo sapiens
5 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
6 ATPIF1 93974
Co-fractionation Homo sapiens
7 ARNT2  
Two-hybrid Homo sapiens
8 PSEN1 5663
Affinity Capture-MS Homo sapiens
9 PRNP 5621
Affinity Capture-MS Homo sapiens
10 RTKN 6242
Affinity Capture-MS Homo sapiens
11 RNF183  
Two-hybrid Homo sapiens
12 PAK1 5058
Affinity Capture-MS Homo sapiens
13 PAK3  
Affinity Capture-MS Homo sapiens
14 PPP1R13L 10848
Affinity Capture-MS Homo sapiens
15 C1orf106  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CDH3 1001
Affinity Capture-MS Homo sapiens
17 ARVCF 421
Affinity Capture-MS Homo sapiens
18 CTNNB1 1499
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
19 CDH15 1013
Affinity Capture-MS Homo sapiens
20 APC  
Affinity Capture-MS Homo sapiens
21 PAK2 5062
Affinity Capture-MS Homo sapiens
22 ACAA2 10449
Co-fractionation Homo sapiens
23 TXN 7295
Co-fractionation Homo sapiens
24 CST1  
Affinity Capture-MS Homo sapiens
25 CYTH1 9267
Affinity Capture-MS Homo sapiens
26 VAMP2 6844
Co-fractionation Homo sapiens
27 UBL3 5412
Affinity Capture-MS Homo sapiens
28 CYTH2 9266
Affinity Capture-MS Homo sapiens
29 FRMD4A  
Affinity Capture-MS Homo sapiens
30 MEF2D  
Affinity Capture-MS Homo sapiens
31 DTX1  
Two-hybrid Homo sapiens
FRET Homo sapiens
32 TCEA2  
Two-hybrid Homo sapiens
33 STAU1 6780
Two-hybrid Homo sapiens
FRET Homo sapiens
34 PPP1CC 5501
Affinity Capture-MS Homo sapiens
35 KBTBD6  
Affinity Capture-MS Homo sapiens
36 UBE2D2 7322
Co-fractionation Homo sapiens
37 CDH1 999
Affinity Capture-MS Homo sapiens
38 GIT1 28964
Affinity Capture-MS Homo sapiens
39 GIT2 9815
Affinity Capture-MS Homo sapiens
40 TMEM65 157378
Co-fractionation Homo sapiens
41 PKP4 8502
Affinity Capture-MS Homo sapiens
42 CIT 11113
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CDK5RAP3 80279
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
44 CDH2 1000
Affinity Capture-MS Homo sapiens
45 PLK4  
Two-hybrid Homo sapiens
46 ARHGEF6 9459
Affinity Capture-MS Homo sapiens
47 FBXO28  
Affinity Capture-MS Homo sapiens
48 CTNND1 1500
Affinity Capture-MS Homo sapiens
49 CTNNA1 1495
Affinity Capture-MS Homo sapiens
50 TANC1 85461
Affinity Capture-MS Homo sapiens
51 TRIM29 23650
Two-hybrid Homo sapiens
52 TANC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 C12orf57 113246
Affinity Capture-MS Homo sapiens
54 RBBP8  
Affinity Capture-MS Homo sapiens
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