Experiment description of studies that identified C12orf57 in sEVs

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for C12orf57

Protein Interactor ExoCarta ID Identification method PubMed Species 1 YWHAB 7529 Affinity Capture-MS Homo sapiens 2 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 3 VPS35 55737 Affinity Capture-MS Homo sapiens 4 CNOT7 29883 Affinity Capture-MS Homo sapiens 5 SNX27 81609 Affinity Capture-MS Homo sapiens 6 PLXNB2 23654 Co-fractionation Homo sapiens 7 VPS29 51699 Affinity Capture-MS Homo sapiens 8 FKBP7 51661 Co-fractionation Homo sapiens 9 SNAP23 8773 Co-fractionation Homo sapiens 10 ARHGAP39   Affinity Capture-MS Homo sapiens 11 COL1A1 1277 Co-fractionation Homo sapiens 12 EGLN3   Affinity Capture-MS Homo sapiens 13 YTHDF1 54915 Affinity Capture-MS Homo sapiens 14 ANKK1   Two-hybrid Homo sapiens 15 LETM1 3954 Co-fractionation Homo sapiens 16 TANC1 85461 Affinity Capture-MS Homo sapiens 17 PPP1CA 5499 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 EMC4 51234 Affinity Capture-MS Homo sapiens 19 AIM1L   Affinity Capture-MS Homo sapiens 20 PALM2   Co-fractionation Homo sapiens 21 ANKRD50 57182 Affinity Capture-MS Homo sapiens 22 AGAP3 116988 Affinity Capture-MS Homo sapiens 23 THAP7   Affinity Capture-MS Homo sapiens 24 PPP1CC 5501 Affinity Capture-MS Homo sapiens 25 CAPZB 832 Affinity Capture-MS Homo sapiens 26 TMEM154   Affinity Capture-MS Homo sapiens 27 TAX1BP3 30851 Affinity Capture-MS Homo sapiens 28 INSL3   Two-hybrid Homo sapiens 29 CCNL1   Affinity Capture-MS Homo sapiens 30 ODF3   Affinity Capture-MS Homo sapiens 31 RPL35A 6165 Affinity Capture-MS Homo sapiens 32 BLMH 642 Affinity Capture-MS Homo sapiens 33 TACC1 6867 Co-fractionation Homo sapiens 34 YIPF3 25844 Affinity Capture-MS Homo sapiens 35 ACO1 48 Co-fractionation Homo sapiens 36 TANC2   Affinity Capture-MS Homo sapiens 37 Polr2a   Two-hybrid Mus musculus 38 SRI 6717 Co-fractionation Homo sapiens View the network

Pathways in which C12orf57 is involved

No pathways found