Gene description for PTPN14
Gene name protein tyrosine phosphatase, non-receptor type 14
Gene symbol PTPN14
Other names/aliases PEZ
PTP36
Species Homo sapiens
 Database cross references - PTPN14
ExoCarta ExoCarta_5784
Vesiclepedia VP_5784
Entrez Gene 5784
HGNC 9647
MIM 603155
UniProt Q15678  
 PTPN14 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for PTPN14
Molecular Function
    transcription coregulator activity GO:0003712 IMP
    protein tyrosine phosphatase activity GO:0004725 IBA
    protein tyrosine phosphatase activity GO:0004725 IMP
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    receptor tyrosine kinase binding GO:0030971 IPI
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    lymphangiogenesis GO:0001946 IBA
    lymphangiogenesis GO:0001946 IMP
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 TAS
    negative regulation of cell population proliferation GO:0008285 IMP
    regulation of protein export from nucleus GO:0046825 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified PTPN14 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PTPN14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACAD8 27034
Affinity Capture-MS Homo sapiens
2 HDAC4  
Affinity Capture-MS Homo sapiens
3 GIGYF1  
Affinity Capture-MS Homo sapiens
4 OCLN 100506658
Proximity Label-MS Homo sapiens
5 CCT8L1P  
Affinity Capture-MS Homo sapiens
6 KSR1  
Affinity Capture-MS Homo sapiens
7 RANBP10 57610
Affinity Capture-MS Homo sapiens
8 MARCKS 4082
Proximity Label-MS Homo sapiens
9 WWP1 11059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 GJA1 2697
Proximity Label-MS Homo sapiens
11 ATE1 11101
Affinity Capture-MS Homo sapiens
12 PARD3 56288
Proximity Label-MS Homo sapiens
13 TRIM66  
Affinity Capture-MS Homo sapiens
14 USP9X 8239
Affinity Capture-MS Homo sapiens
15 UBC 7316
Affinity Capture-MS Homo sapiens
16 VHL  
Affinity Capture-Western Homo sapiens
17 CBY1  
Affinity Capture-MS Homo sapiens
18 WDR26 80232
Affinity Capture-MS Homo sapiens
19 EIF4E2  
Affinity Capture-MS Homo sapiens
20 ANKRD50 57182
Affinity Capture-MS Homo sapiens
21 KIF13B 23303
Affinity Capture-MS Homo sapiens
22 MAGI3 260425
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MLLT4 4301
Proximity Label-MS Homo sapiens
24 ZBTB21  
Affinity Capture-MS Homo sapiens
25 BICD1 636
Proximity Label-MS Homo sapiens
26 ZNF638 27332
Affinity Capture-MS Homo sapiens
27 AMMECR1L 83607
Affinity Capture-MS Homo sapiens
28 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ARMC8 25852
Affinity Capture-MS Homo sapiens
30 LRR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
31 PTPN14 5784
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 LRFN1  
Affinity Capture-MS Homo sapiens
34 CTNNA1 1495
Affinity Capture-MS Homo sapiens
35 RAB35 11021
Proximity Label-MS Homo sapiens
36 CSNK1D 1453
Affinity Capture-MS Homo sapiens
37 EBAG9 9166
Proximity Label-MS Homo sapiens
38 LAMTOR1 55004
Proximity Label-MS Homo sapiens
39 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 KRT8 3856
Proximity Label-MS Homo sapiens
41 S100A2 6273
Affinity Capture-MS Homo sapiens
42 CXADR 1525
Proximity Label-MS Homo sapiens
43 SOCS7  
Affinity Capture-Western Homo sapiens
44 TESK2  
Affinity Capture-MS Homo sapiens
45 LYN 4067
Proximity Label-MS Homo sapiens
46 MCAM 4162
Proximity Label-MS Homo sapiens
47 DNAJC5 80331
Proximity Label-MS Homo sapiens
48 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 BPIFB1 92747
Affinity Capture-MS Homo sapiens
50 PLK1 5347
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 BICD2 23299
Proximity Label-MS Homo sapiens
52 CUL2 8453
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
53 ACAD11 84129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 YAP1 10413
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CRLF3 51379
Affinity Capture-MS Homo sapiens
56 CTDSPL 10217
Proximity Label-MS Homo sapiens
57 RMND5A 64795
Affinity Capture-MS Homo sapiens
58 BAG3 9531
Affinity Capture-MS Homo sapiens
59 LIMA1 51474
Affinity Capture-MS Homo sapiens
60 TJP1 7082
Affinity Capture-MS Homo sapiens
61 STK3 6788
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 MOB1B 92597
Proximity Label-MS Homo sapiens
63 DCLK1 9201
Affinity Capture-MS Homo sapiens
64 PTEN 5728
Affinity Capture-MS Homo sapiens
65 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 RC3H2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
67 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
68 MEX3A  
Affinity Capture-RNA Homo sapiens
69 PRKCZ 5590
Affinity Capture-MS Homo sapiens
70 SRGAP2 23380
Affinity Capture-MS Homo sapiens
71 CUL3 8452
Affinity Capture-MS Homo sapiens
72 YWHAZ 7534
Affinity Capture-MS Homo sapiens
73 ARF6 382
Proximity Label-MS Homo sapiens
74 VBP1 7411
Affinity Capture-MS Homo sapiens
75 KCNE3  
Affinity Capture-MS Homo sapiens
76 GPS1 2873
Affinity Capture-MS Homo sapiens
77 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 HECW2  
Affinity Capture-MS Homo sapiens
79 KCTD3  
Affinity Capture-MS Homo sapiens
80 SAV1  
Proximity Label-MS Homo sapiens
81 WWC1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
82 RHPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 SYDE1 85360
Affinity Capture-MS Homo sapiens
84 CGN  
Affinity Capture-MS Homo sapiens
85 DDB1 1642
Affinity Capture-MS Homo sapiens
86 AMOT 154796
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 Yap1  
Reconstituted Complex Mus musculus
88 RHOB 388
Proximity Label-MS Homo sapiens
89 Rpl35 66489
Affinity Capture-MS Mus musculus
90 HAX1  
Proximity Label-MS Homo sapiens
91 FLOT1 10211
Proximity Label-MS Homo sapiens
92 DENND4C 55667
Affinity Capture-MS Homo sapiens
93 RNF43  
Proximity Label-MS Homo sapiens
94 PDLIM7 9260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 KIF2A 3796
Affinity Capture-MS Homo sapiens
96 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
97 PHF20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 JUP 3728
Affinity Capture-MS Homo sapiens
99 RTKN 6242
Affinity Capture-MS Homo sapiens
100 WWOX 51741
Affinity Capture-MS Homo sapiens
101 PPM1H  
Affinity Capture-MS Homo sapiens
102 EZR 7430
Proximity Label-MS Homo sapiens
103 SRSF12  
Affinity Capture-MS Homo sapiens
104 WWC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 LCK 3932
Proximity Label-MS Homo sapiens
106 LATS2 26524
Proximity Label-MS Homo sapiens
107 DENND1A 57706
Affinity Capture-MS Homo sapiens
108 C11orf52 91894
Proximity Label-MS Homo sapiens
109 LATS1  
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
110 NF1 4763
Affinity Capture-MS Homo sapiens
111 WWP2 11060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 STXBP4 252983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
114 PARD6B 84612
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 INPP5E 56623
Affinity Capture-MS Homo sapiens
116 PXN 5829
Proximity Label-MS Homo sapiens
117 NDEL1 81565
Affinity Capture-MS Homo sapiens
118 EPHA2 1969
Proximity Label-MS Homo sapiens
119 KRAS 3845
Proximity Label-MS Homo sapiens
120 MOB1A 55233
Proximity Label-MS Homo sapiens
121 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
122 MAST3  
Affinity Capture-MS Homo sapiens
123 COPS4 51138
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
125 RPA2 6118
Proximity Label-MS Homo sapiens
126 MAGI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 CAV1 857
Proximity Label-MS Homo sapiens
128 MAEA 10296
Affinity Capture-MS Homo sapiens
129 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here