Gene description for TJP1
Gene name tight junction protein 1
Gene symbol TJP1
Other names/aliases ZO-1
Species Homo sapiens
 Database cross references - TJP1
ExoCarta ExoCarta_7082
Vesiclepedia VP_7082
Entrez Gene 7082
HGNC 11827
MIM 601009
UniProt Q07157  
 TJP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TJP1
Molecular Function
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    cadherin binding GO:0045296 HDA
    cell adhesion molecule binding GO:0050839 IBA
Biological Process
    cell-cell junction assembly GO:0007043 TAS
    positive regulation of cell population proliferation GO:0008284 IMP
    actin cytoskeleton organization GO:0030036 IMP
    positive regulation of cell migration GO:0030335 IMP
    actomyosin structure organization GO:0031032 IMP
    adherens junction maintenance GO:0034334 IMP
    maintenance of blood-brain barrier GO:0035633 NAS
    ameloblast differentiation GO:0036305 ISS
    negative regulation of apoptotic process GO:0043066 IMP
    cell-cell junction organization GO:0045216 IBA
    cell-cell junction organization GO:0045216 IMP
    regulation of cytoskeleton organization GO:0051493 IMP
    negative regulation of stress fiber assembly GO:0051497 ISS
    protein localization to adherens junction GO:0071896 IMP
    establishment of endothelial intestinal barrier GO:0090557 IBA
    establishment of endothelial intestinal barrier GO:0090557 IMP
    cell-cell adhesion GO:0098609 IBA
    protein localization to cell-cell junction GO:0150105 IBA
    protein localization to cell-cell junction GO:0150105 IMP
    regulation of cell junction assembly GO:1901888 IMP
    protein localization to bicellular tight junction GO:1902396 IMP
    positive regulation of sprouting angiogenesis GO:1903672 IMP
    positive regulation of blood-brain barrier permeability GO:1905605 IBA
    positive regulation of blood-brain barrier permeability GO:1905605 IMP
    positive regulation of cell-cell adhesion mediated by cadherin GO:2000049 IMP
    regulation of bicellular tight junction assembly GO:2000810 IMP
Subcellular Localization
    podosome GO:0002102 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    adherens junction GO:0005912 TAS
    gap junction GO:0005921 ISS
    bicellular tight junction GO:0005923 IBA
    bicellular tight junction GO:0005923 IDA
    basolateral plasma membrane GO:0016323 IDA
    cell junction GO:0030054 IDA
    cell junction GO:0030054 TAS
    protein-containing complex GO:0032991 IDA
    cell projection GO:0042995 IEA
    apical junction complex GO:0043296 IDA
    apical part of cell GO:0045177 IDA
    tight junction GO:0070160 IDA
    tight junction GO:0070160 IMP
 Experiment description of studies that identified TJP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
15
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
20
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TJP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 F11R 50848
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 GJD3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 ISG15 9636
Affinity Capture-MS Homo sapiens
5 Cdk1 12534
Affinity Capture-MS Mus musculus
6 NCBP1 4686
Cross-Linking-MS (XL-MS) Homo sapiens
7 PPP1CB 5500
Affinity Capture-MS Homo sapiens
8 KRT1 3848
Affinity Capture-MS Homo sapiens
9 OCLN 100506658
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 VAPA 9218
Co-fractionation Homo sapiens
11 ATG5 9474
Proximity Label-MS Homo sapiens
12 GJA1 2697
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 PARD3 56288
Co-localization Homo sapiens
Proximity Label-MS Homo sapiens
14 EPB41L5 57669
Affinity Capture-MS Homo sapiens
15 SFPQ 6421
Co-fractionation Homo sapiens
16 TCF7L2  
Affinity Capture-MS Homo sapiens
17 LUZP4  
Affinity Capture-MS Homo sapiens
18 FAM168A  
Affinity Capture-MS Homo sapiens
19 ACTN1 87
Affinity Capture-MS Homo sapiens
20 ANXA6 309
Affinity Capture-MS Homo sapiens
21 Actb 11461
Affinity Capture-MS Mus musculus
22 RTN4 57142
Co-fractionation Homo sapiens
23 MARVELD2 153562
Co-localization Homo sapiens
24 GRHPR 9380
Co-fractionation Homo sapiens
25 KIF20A 10112
Affinity Capture-MS Homo sapiens
26 STRN 6801
Affinity Capture-Western Homo sapiens
27 KIF23 9493
Affinity Capture-MS Homo sapiens
28 MCM2 4171
Co-fractionation Homo sapiens
29 ACTR3 10096
Proximity Label-MS Homo sapiens
30 COPB2 9276
Co-fractionation Homo sapiens
31 SRSF10 10772
Co-fractionation Homo sapiens
32 FBXW7  
Affinity Capture-MS Homo sapiens
33 PRC1 9055
Affinity Capture-MS Homo sapiens
34 CALD1 800
Affinity Capture-MS Homo sapiens
35 CALM3 808
Affinity Capture-MS Homo sapiens
36 KHDRBS1 10657
Protein-peptide Homo sapiens
37 OBSL1 23363
Affinity Capture-MS Homo sapiens
38 DDX58 23586
Affinity Capture-RNA Homo sapiens
39 C1orf111  
Affinity Capture-MS Homo sapiens
40 WDR3  
Co-fractionation Homo sapiens
41 CLDN6 9074
Reconstituted Complex Homo sapiens
42 CTTN 2017
Affinity Capture-MS Homo sapiens
43 PTPN14 5784
Affinity Capture-MS Homo sapiens
44 RAB8B 51762
Affinity Capture-Western Homo sapiens
45 MECP2 4204
Affinity Capture-MS Homo sapiens
46 SNX27 81609
Affinity Capture-MS Homo sapiens
47 CSNK2B 1460
Affinity Capture-MS Homo sapiens
48 PCF11 51585
Co-fractionation Homo sapiens
49 KIF14 9928
Affinity Capture-MS Homo sapiens
50 NOTCH2 4853
Co-fractionation Homo sapiens
51 PFN1 5216
Proximity Label-MS Homo sapiens
52 KRT8 3856
Affinity Capture-MS Homo sapiens
53 TOLLIP 54472
Proximity Label-MS Homo sapiens
54 CLDN8  
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
55 CLDN7 1366
Reconstituted Complex Homo sapiens
56 MIB1 57534
Proximity Label-MS Homo sapiens
Co-localization Homo sapiens
57 PPIE 10450
Affinity Capture-MS Homo sapiens
58 JAM3 83700
Reconstituted Complex Homo sapiens
59 JAM2  
Reconstituted Complex Homo sapiens
60 CXADR 1525
Proximity Label-MS Homo sapiens
61 CERS2 29956
Affinity Capture-MS Homo sapiens
62 KRT10 3858
Affinity Capture-MS Homo sapiens
63 SOWAHB  
Affinity Capture-MS Homo sapiens
64 CDH1 999
Proximity Label-MS Homo sapiens
65 CHMP4B 128866
Affinity Capture-MS Homo sapiens
66 YAP1 10413
Affinity Capture-MS Homo sapiens
67 VAPB 9217
Co-fractionation Homo sapiens
68 SUN2 25777
Affinity Capture-MS Homo sapiens
69 RNF166  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 U2AF2 11338
Co-fractionation Homo sapiens
71 BICD2 23299
Proximity Label-MS Homo sapiens
72 RPA4  
Proximity Label-MS Homo sapiens
73 ACTB 60
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 TUBA1C 84790
Affinity Capture-MS Homo sapiens
75 IFI30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 LATS2 26524
Proximity Label-MS Homo sapiens
77 CTDSPL 10217
Proximity Label-MS Homo sapiens
78 KIF2C 11004
Affinity Capture-MS Homo sapiens
79 VMP1 81671
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
80 C1orf189  
Affinity Capture-MS Homo sapiens
81 ZPR1 8882
Co-fractionation Homo sapiens
82 ANLN 54443
Affinity Capture-MS Homo sapiens
83 CDC14A  
Proximity Label-MS Homo sapiens
84 PHLPP1  
Proximity Label-MS Homo sapiens
85 GJA8  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
86 NTRK1 4914
Affinity Capture-MS Homo sapiens
87 TUBB 203068
Affinity Capture-MS Homo sapiens
88 SUGP1  
Co-fractionation Homo sapiens
89 Myh9 17886
Affinity Capture-MS Mus musculus
90 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 DCAF7 10238
Affinity Capture-MS Homo sapiens
92 ANGPT1 284
Affinity Capture-MS Homo sapiens
93 XPO1 7514
Affinity Capture-MS Homo sapiens
94 PSENEN  
Affinity Capture-MS Homo sapiens
95 ECT2 1894
Affinity Capture-MS Homo sapiens
96 SIRT7  
Affinity Capture-MS Homo sapiens
97 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
98 UQCRB 7381
Affinity Capture-MS Homo sapiens
99 ERLEC1 27248
Affinity Capture-MS Homo sapiens
100 BTF3 689
Affinity Capture-MS Homo sapiens
101 EGFR 1956
Affinity Capture-Western Homo sapiens
102 MAB21L2  
Affinity Capture-MS Homo sapiens
103 MYO19  
Affinity Capture-MS Homo sapiens
104 THOC5 8563
Co-fractionation Homo sapiens
105 TRIM68 55128
Affinity Capture-MS Homo sapiens
106 GJA3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
107 Mphosph9  
Affinity Capture-MS Mus musculus
108 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 MYO1C 4641
Affinity Capture-MS Homo sapiens
110 HOOK3 84376
Proximity Label-MS Homo sapiens
111 PRKCI 5584
Co-localization Homo sapiens
112 CLDN5 7122
Reconstituted Complex Homo sapiens
113 KIRREL 55243
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
114 PCBP1 5093
Proximity Label-MS Homo sapiens
115 TJP2 9414
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
116 RAPGEF2 9693
Affinity Capture-MS Homo sapiens
117 MYC  
Affinity Capture-MS Homo sapiens
118 CRYBA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 DLST 1743
Affinity Capture-MS Homo sapiens
120 LGALS14  
Affinity Capture-MS Homo sapiens
121 ACTN4 81
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
122 CGN  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 BICD1 636
Proximity Label-MS Homo sapiens
124 AMOT 154796
Proximity Label-MS Homo sapiens
125 Yap1  
Reconstituted Complex Mus musculus
126 RHOB 388
Proximity Label-MS Homo sapiens
127 Rpl35 66489
Affinity Capture-MS Mus musculus
128 CTNNA1 1495
Proximity Label-MS Homo sapiens
129 NDUFV2 4729
Co-fractionation Homo sapiens
130 CCDC102B  
Affinity Capture-MS Homo sapiens
131 RAE1 8480
Affinity Capture-MS Homo sapiens
132 CCDC8  
Affinity Capture-MS Homo sapiens
133 SUMO1 7341
Affinity Capture-MS Homo sapiens
134 CLDN2 9075
Reconstituted Complex Homo sapiens
135 RNF43  
Proximity Label-MS Homo sapiens
136 NUDT21 11051
Co-fractionation Homo sapiens
137 SSTR3  
Affinity Capture-MS Homo sapiens
138 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
139 SPTAN1 6709
Affinity Capture-MS Homo sapiens
140 FLNA 2316
Affinity Capture-MS Homo sapiens
141 PLEC 5339
Affinity Capture-MS Homo sapiens
142 HSPA8 3312
Co-fractionation Homo sapiens
143 PIN1 5300
Co-fractionation Homo sapiens
144 TJP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
145 EZR 7430
Proximity Label-MS Homo sapiens
146 PTBP1 5725
Co-fractionation Homo sapiens
147 CLDN3 1365
Reconstituted Complex Homo sapiens
148 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
149 CAPZB 832
Affinity Capture-MS Homo sapiens
150 HSP90AA1 3320
Reconstituted Complex Homo sapiens
151 CLDN1 9076
Reconstituted Complex Homo sapiens
152 TUBB4A 10382
Affinity Capture-MS Homo sapiens
153 LATS1  
Proximity Label-MS Homo sapiens
154 MAGIX 79917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
155 ERCC3  
Co-fractionation Homo sapiens
156 WWP2 11060
Affinity Capture-MS Homo sapiens
157 TRIM37  
Proximity Label-MS Homo sapiens
158 DHX16 8449
Co-fractionation Homo sapiens
159 PSMA5 5686
Co-localization Homo sapiens
160 YBX3 8531
Reconstituted Complex Homo sapiens
161 CEP135  
Proximity Label-MS Homo sapiens
162 CIT 11113
Affinity Capture-MS Homo sapiens
163 FLOT1 10211
Proximity Label-MS Homo sapiens
164 ZUFSP 221302
Affinity Capture-MS Homo sapiens
165 CUL7 9820
Affinity Capture-MS Homo sapiens
166 KRAS 3845
Proximity Label-MS Homo sapiens
167 AR 367
Affinity Capture-MS Homo sapiens
168 MLLT4 4301
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
169 CLDN4 1364
Reconstituted Complex Homo sapiens
170 VIM 7431
Affinity Capture-MS Homo sapiens
171 PARD6A  
Co-localization Homo sapiens
172 CCR1  
Affinity Capture-MS Homo sapiens
173 RPA3 6119
Proximity Label-MS Homo sapiens
174 NDUFS6  
Co-fractionation Homo sapiens
175 FAM133A 286499
Affinity Capture-MS Homo sapiens
176 SCO2  
Co-fractionation Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here