Gene description for CHD5
Gene name chromodomain helicase DNA binding protein 5
Gene symbol CHD5
Other names/aliases CHD-5
Species Homo sapiens
 Database cross references - CHD5
ExoCarta ExoCarta_26038
Vesiclepedia VP_26038
Entrez Gene 26038
HGNC 16816
MIM 610771
UniProt Q8TDI0  
 CHD5 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for CHD5
Molecular Function
    DNA binding GO:0003677 IBA
    chromatin binding GO:0003682 IBA
    DNA clamp loader activity GO:0003689 IEA
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IBA
    double-stranded DNA helicase activity GO:0036121 IEA
    histone binding GO:0042393 IBA
    metal ion binding GO:0046872 IEA
    H3K27me3 modified histone binding GO:0061628 IDA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent chromatin remodeler activity GO:0140658 IBA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    chromatin remodeling GO:0006338 IBA
    chromatin remodeling GO:0006338 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    negative regulation of cell population proliferation GO:0008285 ISS
    cerebral cortex neuron differentiation GO:0021895 ISS
    DNA duplex unwinding GO:0032508 IEA
    sperm DNA condensation GO:0035092 ISS
    chromatin looping GO:0140588 IEA
    positive regulation of signal transduction by p53 class mediator GO:1901798 ISS
Subcellular Localization
    chromatin GO:0000785 IBA
    heterochromatin GO:0000792 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    NuRD complex GO:0016581 IBA
    NuRD complex GO:0016581 ISS
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified CHD5 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CHD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF219  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 GATAD2A 54815
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 BCL11A 53335
Affinity Capture-MS Homo sapiens
4 GATAD2B 57459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CHD3 1107
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
6 HDAC2 3066
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
7 WDR77 79084
Affinity Capture-MS Homo sapiens
8 BCL11B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SAP130  
Affinity Capture-MS Homo sapiens
10 PHF12  
Affinity Capture-MS Homo sapiens
11 BAG2 9532
Affinity Capture-MS Homo sapiens
12 MTA1 9112
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
13 SOX15  
Proximity Label-MS Homo sapiens
14 SUB1 10923
Co-fractionation Homo sapiens
15 ADNP2  
Affinity Capture-MS Homo sapiens
16 RBBP7 5931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
17 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
18 SHPRH  
Affinity Capture-MS Homo sapiens
19 MBD3 53615
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
20 ZBTB2 57621
Affinity Capture-MS Homo sapiens
21 SAP30 8819
Affinity Capture-MS Homo sapiens
22 CHD4 1108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-Western Homo sapiens
23 C17orf49 124944
Affinity Capture-MS Homo sapiens
24 BRMS1L  
Affinity Capture-MS Homo sapiens
25 HIST3H3 8290
Affinity Capture-Western Homo sapiens
26 HDAC1 3065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
27 HMGXB4 10042
Affinity Capture-MS Homo sapiens
28 CBX3 11335
Affinity Capture-MS Homo sapiens
29 LIN9  
Affinity Capture-MS Homo sapiens
30 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
31 MTA3 57504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 HSPA2 3306
Affinity Capture-MS Homo sapiens
33 ZMYND8 23613
Affinity Capture-MS Homo sapiens
34 EEF1A1P5 158078
Cross-Linking-MS (XL-MS) Homo sapiens
35 BPTF 2186
Affinity Capture-MS Homo sapiens
36 CDK2AP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MBD2 8932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MYBL2 4605
Affinity Capture-MS Homo sapiens
39 PRC1 9055
Affinity Capture-MS Homo sapiens
40 DNAH10 196385
Cross-Linking-MS (XL-MS) Homo sapiens
41 ARID4B  
Affinity Capture-MS Homo sapiens
42 MBD3L1  
Affinity Capture-MS Homo sapiens
43 SUDS3  
Affinity Capture-MS Homo sapiens
44 CHAF1B  
Affinity Capture-MS Homo sapiens
45 PWWP2A  
Affinity Capture-MS Homo sapiens
46 RBBP4 5928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 MBD3L2  
Affinity Capture-MS Homo sapiens
48 LIN37  
Affinity Capture-MS Homo sapiens
49 SIN3B  
Affinity Capture-MS Homo sapiens
50 EIF5 1983
Co-fractionation Homo sapiens
51 RAD51B  
Cross-Linking-MS (XL-MS) Homo sapiens
52 MTA2 9219
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which CHD5 is involved
No pathways found





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