Experiment description of studies that identified EEF1A1P5 in sEVs

1 Experiment ID 494 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 20 MISEV standards ✔ EM Biophysical techniques ✔ HSP90|CD63|CD81|LAMP1 Enriched markers ✔ GOLGA2|cytochrome c Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17956143     Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells. Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS" Journal name JPR Publication year 2007 Sample Colorectal cancer cells Sample name HT29 Isolation/purification methods Differential centrifugation Sucrose density gradient Diafiltration Flotation density 1.16 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

4 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for EEF1A1P5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PTMS 5763 Cross-Linking-MS (XL-MS) Homo sapiens 2 SHC1 6464 Affinity Capture-MS Homo sapiens 3 RPS3A 6189 Cross-Linking-MS (XL-MS) Homo sapiens 4 BTF3 689 Affinity Capture-MS Homo sapiens 5 USP5 8078 Cross-Linking-MS (XL-MS) Homo sapiens 6 KIF20A 10112 Affinity Capture-MS Homo sapiens 7 MRPL52   Cross-Linking-MS (XL-MS) Homo sapiens 8 PYGO1   Cross-Linking-MS (XL-MS) Homo sapiens 9 ARL8B 55207 Cross-Linking-MS (XL-MS) Homo sapiens 10 LAMC1 3915 Cross-Linking-MS (XL-MS) Homo sapiens 11 TRIM28 10155 Cross-Linking-MS (XL-MS) Homo sapiens 12 KIF23 9493 Affinity Capture-MS Homo sapiens 13 MCM2 4171 Affinity Capture-MS Homo sapiens 14 NAV1 89796 Cross-Linking-MS (XL-MS) Homo sapiens 15 TLN1 7094 Cross-Linking-MS (XL-MS) Homo sapiens 16 PRC1 9055 Affinity Capture-MS Homo sapiens 17 FER1L6   Cross-Linking-MS (XL-MS) Homo sapiens 18 ITGA4 3676 Affinity Capture-MS Homo sapiens 19 EEF1G 1937 Cross-Linking-MS (XL-MS) Homo sapiens 20 PGK2 5232 Cross-Linking-MS (XL-MS) Homo sapiens 21 ADARB2   Cross-Linking-MS (XL-MS) Homo sapiens 22 KIF14 9928 Affinity Capture-MS Homo sapiens 23 PPP5C 5536 Cross-Linking-MS (XL-MS) Homo sapiens 24 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 25 DDX21 9188 Cross-Linking-MS (XL-MS) Homo sapiens 26 PARK2   Affinity Capture-MS Homo sapiens 27 RPS10 6204 Cross-Linking-MS (XL-MS) Homo sapiens 28 NONO 4841 Cross-Linking-MS (XL-MS) Homo sapiens 29 PSMD14 10213 Affinity Capture-MS Homo sapiens 30 UBE2I 7329 Cross-Linking-MS (XL-MS) Homo sapiens 31 UBASH3B 84959 Affinity Capture-MS Homo sapiens 32 ESRRB   Affinity Capture-MS Homo sapiens 33 CHMP4B 128866 Affinity Capture-MS Homo sapiens 34 TTN 7273 Cross-Linking-MS (XL-MS) Homo sapiens 35 CD4 920 Cross-Linking-MS (XL-MS) Homo sapiens 36 IFI16 3428 Affinity Capture-MS Homo sapiens 37 U2AF2 11338 Affinity Capture-MS Homo sapiens 38 MIA3 375056 Cross-Linking-MS (XL-MS) Homo sapiens 39 ENO1 2023 Cross-Linking-MS (XL-MS) Homo sapiens 40 MYH10 4628 Cross-Linking-MS (XL-MS) Homo sapiens 41 GVINP1   Cross-Linking-MS (XL-MS) Homo sapiens 42 HIST1H2BK 85236 Cross-Linking-MS (XL-MS) Homo sapiens 43 ARPC1A 10552 Cross-Linking-MS (XL-MS) Homo sapiens 44 PRKACB 5567 Cross-Linking-MS (XL-MS) Homo sapiens 45 AIMP2 7965 Affinity Capture-MS Homo sapiens 46 ANLN 54443 Affinity Capture-MS Homo sapiens 47 TRIM21 6737 Affinity Capture-MS Homo sapiens 48 GAN 8139 Affinity Capture-MS Homo sapiens 49 PSMA3 5684 Affinity Capture-MS Homo sapiens 50 FANCD2   Affinity Capture-MS Homo sapiens 51 UGGT1 56886 Cross-Linking-MS (XL-MS) Homo sapiens 52 ECT2 1894 Affinity Capture-MS Homo sapiens 53 ANXA4 307 Cross-Linking-MS (XL-MS) Homo sapiens 54 NR3C1 2908 Affinity Capture-MS Homo sapiens 55 ETF1 2107 Cross-Linking-MS (XL-MS) Homo sapiens 56 EPRS 2058 Cross-Linking-MS (XL-MS) Homo sapiens 57 ZC3H11A   Cross-Linking-MS (XL-MS) Homo sapiens 58 AURKB 9212 Affinity Capture-MS Homo sapiens 59 SLC35B2 347734 Cross-Linking-MS (XL-MS) Homo sapiens 60 GNAS 2778 Cross-Linking-MS (XL-MS) Homo sapiens 61 IGSF3 3321 Cross-Linking-MS (XL-MS) Homo sapiens 62 RPL12 6136 Cross-Linking-MS (XL-MS) Homo sapiens 63 EEF1A2 1917 Cross-Linking-MS (XL-MS) Homo sapiens 64 EEF1B2 1933 Cross-Linking-MS (XL-MS) Homo sapiens 65 DYRK2 8445 Affinity Capture-MS Homo sapiens 66 DDRGK1 65992 Affinity Capture-MS Homo sapiens 67 SGTA 6449 Cross-Linking-MS (XL-MS) Homo sapiens 68 MSN 4478 Cross-Linking-MS (XL-MS) Homo sapiens 69 HEATR1 55127 Cross-Linking-MS (XL-MS) Homo sapiens 70 CHD5 26038 Cross-Linking-MS (XL-MS) Homo sapiens 71 GCN1L1 10985 Cross-Linking-MS (XL-MS) Homo sapiens 72 H2AFX 3014 Cross-Linking-MS (XL-MS) Homo sapiens 73 TXLNA 200081 Cross-Linking-MS (XL-MS) Homo sapiens 74 IKBKAP 8518 Cross-Linking-MS (XL-MS) Homo sapiens 75 EIF3F 8665 Affinity Capture-MS Homo sapiens 76 PRKCSH 5589 Cross-Linking-MS (XL-MS) Homo sapiens 77 SPOP   Affinity Capture-MS Homo sapiens 78 RPS25 6230 Cross-Linking-MS (XL-MS) Homo sapiens 79 GRB2 2885 Affinity Capture-MS Homo sapiens 80 TP53 7157 Affinity Capture-MS Homo sapiens 81 GPR158   Cross-Linking-MS (XL-MS) Homo sapiens 82 EIF1AX 1964 Cross-Linking-MS (XL-MS) Homo sapiens 83 DNAJA2 10294 Cross-Linking-MS (XL-MS) Homo sapiens 84 MCM5 4174 Affinity Capture-MS Homo sapiens 85 SMARCC1 6599 Cross-Linking-MS (XL-MS) Homo sapiens 86 PLXNA1 5361 Cross-Linking-MS (XL-MS) Homo sapiens 87 HIST1H2BC 8347 Cross-Linking-MS (XL-MS) Homo sapiens 88 HIST1H1E 3008 Cross-Linking-MS (XL-MS) Homo sapiens 89 RPS5 6193 Cross-Linking-MS (XL-MS) Homo sapiens 90 UFL1 23376 Affinity Capture-MS Homo sapiens 91 HDGFRP2 84717 Cross-Linking-MS (XL-MS) Homo sapiens 92 EIF3J 8669 Cross-Linking-MS (XL-MS) Homo sapiens 93 CYLD   Affinity Capture-MS Homo sapiens 94 TRIM31   Affinity Capture-MS Homo sapiens 95 PINK1   Affinity Capture-MS Homo sapiens 96 SERBP1 26135 Affinity Capture-MS Homo sapiens 97 C9orf72   Affinity Capture-MS Homo sapiens 98 CDC37 11140 Affinity Capture-MS Homo sapiens View the network

Pathways in which EEF1A1P5 is involved

No pathways found