ExoCarta: Gene summary

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Gene description for MIA3
Gene name	melanoma inhibitory activity family, member 3
Gene symbol	MIA3
Other names/aliases	ARNT D320 TANGO TANGO1 UNQ6077
Species	Homo sapiens
Database cross references - MIA3
ExoCarta	ExoCarta_375056
Vesiclepedia	VP_375056
Entrez Gene	375056
HGNC	24008
MIM	613455
UniProt	Q5JRA6
MIA3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Retinal pigment epithelial cells	35333565
Retinal pigment epithelial cells	35333565

Gene ontology annotations for MIA3

Molecular Function
	protein binding	GO:0005515	IPI
	cargo receptor activity	GO:0038024	IMP
Biological Process
	cell migration involved in sprouting angiogenesis	GO:0002042	IMP
	positive regulation of leukocyte migration	GO:0002687	IDA
	exocytosis	GO:0006887	IMP
	endoplasmic reticulum to Golgi vesicle-mediated transport	GO:0006888	IBA
	endoplasmic reticulum to Golgi vesicle-mediated transport	GO:0006888	IMP
	endoplasmic reticulum organization	GO:0007029	IMP
	negative regulation of cell adhesion	GO:0007162	IDA
	protein secretion	GO:0009306	IBA
	protein transport	GO:0015031	IMP
	negative regulation of cell migration	GO:0030336	IDA
	vesicle cargo loading	GO:0035459	IBA
	wound healing	GO:0042060	IDA
	lipoprotein transport	GO:0042953	IMP
	protein localization to endoplasmic reticulum exit site	GO:0070973	IMP
	COPII-coated vesicle cargo loading	GO:0090110	IMP
	cellular response to oxidised low-density lipoprotein particle stimulus	GO:0140052	IMP
	negative regulation of leukocyte cell-cell adhesion	GO:1903038	IMP
	negative regulation of lymphocyte migration	GO:2000402	IMP
Subcellular Localization
	endoplasmic reticulum lumen	GO:0005788	TAS
	endoplasmic reticulum membrane	GO:0005789	IBA
	endoplasmic reticulum membrane	GO:0005789	IDA
	endoplasmic reticulum membrane	GO:0005789	TAS
	membrane	GO:0016020	HDA
	membrane	GO:0016020	IDA
	intracellular membrane-bounded organelle	GO:0043231	IDA
	endoplasmic reticulum exit site	GO:0070971	IBA
	endoplasmic reticulum exit site	GO:0070971	IDA

Experiment description of studies that identified MIA3 in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

834

MISEV standards
✔ EM	Biophysical techniques
✔ LAMP2\|CD63\|FLOT1\|ITGA2B\|ICAM1\|CD9\|CD151\|TFRC\|RAB5A\|GAPDH\|AQP1\|TSG101	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35333565

Organism

Homo sapiens

Experiment description

LAMP2A regulates the loading of proteins into exosomes

Authors

"Ferreira JV, da Rosa Soares A, Ramalho J, MÃ¡ximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, GirÃ£o H, van Niel G, Pereira P"

Journal name

Sci Adv

Publication year

2022

Sample

Retinal pigment epithelial cells

Sample name

ARPE-19

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

835

MISEV standards
✔ EM	Biophysical techniques
✔ CD63\|FLOT1\|ITGA2B\|ICAM1\|CD9\|CD151\|TFRC\|RAB5A\|GAPDH\|AQP1\|TSG101	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35333565

Organism

Homo sapiens

Experiment description

LAMP2A regulates the loading of proteins into exosomes

Authors

"Ferreira JV, da Rosa Soares A, Ramalho J, MÃ¡ximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, GirÃ£o H, van Niel G, Pereira P"

Journal name

Sci Adv

Publication year

2022

Sample

Retinal pigment epithelial cells

Sample name

ARPE-19

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Protein-protein interactions for MIA3

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

TTYH1

Affinity Capture-MS

Homo sapiens

GJD3

Proximity Label-MS

Homo sapiens

CTAGE7P

Affinity Capture-MS

Homo sapiens

CTAGE8

Cross-Linking-MS (XL-MS)

Homo sapiens

OCLN

100506658

Affinity Capture-MS

Homo sapiens

FAM167A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

METTL7A

25840

Proximity Label-MS

Homo sapiens

SYCE1

Affinity Capture-MS

Homo sapiens

GJA1

2697

Proximity Label-MS

Homo sapiens

SRPRB

58477

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CFC1

Affinity Capture-MS

Homo sapiens

NOP56

10528

Proximity Label-MS

Homo sapiens

HSPA13

6782

Proximity Label-MS

Homo sapiens

ECEL1

Affinity Capture-MS

Homo sapiens

HSD17B11

51170

Proximity Label-MS

Homo sapiens

PXN

5829

Proximity Label-MS

Homo sapiens

EYA2

Proximity Label-MS

Homo sapiens

DNAJC25

548645

Proximity Label-MS

Homo sapiens

LRRC59

55379

Proximity Label-MS

Homo sapiens

PTPN1

5770

Proximity Label-MS

Homo sapiens

CLTB

1212

Affinity Capture-MS

Homo sapiens

STX4

6810

Proximity Label-MS

Homo sapiens

KIAA1429

25962

Affinity Capture-MS

Homo sapiens

CLIC3

9022

Two-hybrid

Homo sapiens

GXYLT1

283464

Affinity Capture-MS

Homo sapiens

B3GAT1

Proximity Label-MS

Homo sapiens

SDF2L1

23753

Affinity Capture-MS

Homo sapiens

NCR3

Affinity Capture-MS

Homo sapiens

SERPINA5

5104

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SSR1

6745

Proximity Label-MS

Homo sapiens

MARCH5

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPN2

6185

Proximity Label-MS

Homo sapiens

CTAGE6

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DNAJC16

Proximity Label-MS

Homo sapiens

SLC25A46

91137

Proximity Label-MS

Homo sapiens

P3H1

64175

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GPRC5B

51704

Affinity Capture-MS

Homo sapiens

CALR

811

Cross-Linking-MS (XL-MS)

Homo sapiens

SIGLEC12

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SEC23A

10484

Two-hybrid

Homo sapiens

TMEM106A

Affinity Capture-MS

Homo sapiens

CTAGE5

4253

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens

SERPINA7

6906

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

LMAN1

3998

Proximity Label-MS

Homo sapiens

MTNR1B

Two-hybrid

Homo sapiens

NUP155

9631

Proximity Label-MS

Homo sapiens

KIAA1715

80856

Proximity Label-MS

Homo sapiens

PXMP2

Proximity Label-MS

Homo sapiens

KRT8

3856

Proximity Label-MS

Homo sapiens

FAM19A4

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

COLEC10

10584

Affinity Capture-MS

Homo sapiens

ZNF444

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CEP55

55165

Two-hybrid

Homo sapiens

EMD

2010

Proximity Label-MS

Homo sapiens

QPRT

23475

Affinity Capture-MS

Homo sapiens

ANO4

121601

Affinity Capture-MS

Homo sapiens

RAB4A

5867

Proximity Label-MS

Homo sapiens

DIABLO

56616

Proximity Label-MS

Homo sapiens

MTMR7

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HAO2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NTRK1

4914

Affinity Capture-MS

Homo sapiens

EEF1A1P5

158078

Cross-Linking-MS (XL-MS)

Homo sapiens

DNAJB9

4189

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

STX6

10228

Proximity Label-MS

Homo sapiens

RPN1

6184

Proximity Label-MS

Homo sapiens

DNAJC30

Proximity Label-MS

Homo sapiens

LATS2

26524

Proximity Label-MS

Homo sapiens

RHOT2

89941

Proximity Label-MS

Homo sapiens

VPS18

57617

Affinity Capture-MS

Homo sapiens

SEC61B

10952

Proximity Label-MS

Homo sapiens

DNAJB11

51726

Proximity Label-MS

Homo sapiens

RMDN3

55177

Proximity Label-MS

Homo sapiens

FSTL1

11167

Cross-Linking-MS (XL-MS)

Homo sapiens

REEP5

7905

Proximity Label-MS

Homo sapiens

ZRANB1

54764

Affinity Capture-MS

Homo sapiens

PRG2

Affinity Capture-MS

Homo sapiens

HSPA5

3309

Proximity Label-MS

Homo sapiens

ANO6

196527

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CACNA1A

Two-hybrid

Homo sapiens

MINOS1

Affinity Capture-MS

Homo sapiens

MTMR14

64419

Affinity Capture-MS

Homo sapiens

TPI1

7167

Cross-Linking-MS (XL-MS)

Homo sapiens

SORT1

6272

Affinity Capture-MS

Homo sapiens

DNAJB12

54788

Proximity Label-MS

Homo sapiens

CALR3

Proximity Label-MS

Homo sapiens

PANX1

24145

Proximity Label-MS

Homo sapiens

SLC39A5

283375

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GGH

8836

Affinity Capture-MS

Homo sapiens

APOB

338

Affinity Capture-Western		Homo sapiens
Co-localization		Homo sapiens

BCAP31

10134

Proximity Label-MS

Homo sapiens

HSD3B7

80270

Proximity Label-MS

Homo sapiens

STIM1

6786

Proximity Label-MS

Homo sapiens

FKBP8

23770

Proximity Label-MS

Homo sapiens

PRTN3

5657

Affinity Capture-MS

Homo sapiens

RIPK4

Affinity Capture-MS

Homo sapiens

Evpl

Affinity Capture-MS

Mus musculus

PTPRN

Proximity Label-MS

Homo sapiens

GPR114

Affinity Capture-MS

Homo sapiens

RPA3

6119

Proximity Label-MS

Homo sapiens

100

NIN

51199

Proximity Label-MS

Homo sapiens

101

ATP2A1

487

Proximity Label-MS

Homo sapiens

102

FBXO2

26232

Affinity Capture-MS

Homo sapiens

103

SAV1

Proximity Label-MS

Homo sapiens

104

CRTAP

10491

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

105

RAB9A

9367

Proximity Label-MS

Homo sapiens

106

TRIM27

Two-hybrid

Homo sapiens

107

ASPH

444

Affinity Capture-MS

Homo sapiens

108

NDC80

10403

Proximity Label-MS

Homo sapiens

109

DNAJC10

54431

Proximity Label-MS

Homo sapiens

110

DNAJC3

5611

Proximity Label-MS

Homo sapiens

111

AMOT

154796

Proximity Label-MS

Homo sapiens

112

NXPH3

Affinity Capture-MS

Homo sapiens

113

CYP2C9

Proximity Label-MS

Homo sapiens

114

AKAP1

8165

Proximity Label-MS

Homo sapiens

115

TACC3

Affinity Capture-MS

Homo sapiens

116

BACE2

25825

Affinity Capture-MS

Homo sapiens

117

ADAM30

11085

Affinity Capture-MS

Homo sapiens

118

TMEM87A

25963

Affinity Capture-MS

Homo sapiens

119

RNF43

Proximity Label-MS

Homo sapiens

120

SPCS3

60559

Affinity Capture-MS

Homo sapiens

121

EDN3

Affinity Capture-MS

Homo sapiens

122

VNN2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

123

SIRT6

Affinity Capture-MS

Homo sapiens

124

SERPINA12

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

125

HLA-DRB1

3123

Affinity Capture-MS

Homo sapiens

126

GABRE

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

127

SIDT2

51092

Affinity Capture-MS

Homo sapiens

128

PRDM14

Two-hybrid

Homo sapiens

129

UQCC1

55245

Affinity Capture-MS

Homo sapiens

130

WWOX

51741

Affinity Capture-MS

Homo sapiens

131

PDIA4

9601

Proximity Label-MS

Homo sapiens

132

TALDO1

6888

Cross-Linking-MS (XL-MS)

Homo sapiens

133

CALU

813

Proximity Label-MS

Homo sapiens

134

SEC11A

23478

Affinity Capture-MS

Homo sapiens

135

SLC39A7

7922

Affinity Capture-MS

Homo sapiens

136

LENG8

Two-hybrid

Homo sapiens

137

HLA-DRA

3122

Affinity Capture-MS

Homo sapiens

138

SERPINA3

Affinity Capture-MS

Homo sapiens

139

ERGIC1

57222

Proximity Label-MS

Homo sapiens

140

MAP7D3

79649

Cross-Linking-MS (XL-MS)

Homo sapiens

141

PPFIBP2

8495

Affinity Capture-MS

Homo sapiens

142

LATS1

Proximity Label-MS

Homo sapiens

143

BRK1

55845

Affinity Capture-MS

Homo sapiens

144

RAB5A

5868

Proximity Label-MS

Homo sapiens

145

ERGIC2

51290

Proximity Label-MS

Homo sapiens

146

SIRPD

Affinity Capture-MS

Homo sapiens

147

SEC62

7095

Proximity Label-MS

Homo sapiens

148

SPCS2

9789

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

149

CEP135

Proximity Label-MS

Homo sapiens

150

CLTA

1211

Proximity Label-MS

Homo sapiens

151

HNRNPA1L2

144983

Affinity Capture-MS

Homo sapiens

152

DNAJC1

64215

Proximity Label-MS

Homo sapiens

153

SERPINA10

51156

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

154

CKAP4

10970

Proximity Label-MS

Homo sapiens

155

FXR2

9513

Two-hybrid

Homo sapiens

156

DERL1

79139

Proximity Label-MS

Homo sapiens

View the network

Pathways in which MIA3 is involved

Pathway	Evidence	Source
Asparagine N-linked glycosylation	TAS	Reactome
Cargo concentration in the ER	TAS	Reactome
ER to Golgi Anterograde Transport	TAS	Reactome
Membrane Trafficking	TAS	Reactome
Metabolism of proteins	TAS	Reactome
Post-translational protein modification	TAS	Reactome
Post-translational protein phosphorylation	TAS	Reactome
Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)	TAS	Reactome
Transport to the Golgi and subsequent modification	TAS	Reactome
Vesicle-mediated transport	TAS	Reactome

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