Gene description for DNAJB12
Gene name DnaJ (Hsp40) homolog, subfamily B, member 12
Gene symbol DNAJB12
Other names/aliases DJ10
Species Homo sapiens
 Database cross references - DNAJB12
ExoCarta ExoCarta_54788
Vesiclepedia VP_54788
Entrez Gene 54788
HGNC 14891
MIM 608376
UniProt Q9NXW2  
 DNAJB12 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DNAJB12
Molecular Function
    Hsp70 protein binding GO:0030544 IBA
    Hsp70 protein binding GO:0030544 IPI
Biological Process
    ERAD pathway GO:0036503 IDA
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    chaperone cofactor-dependent protein refolding GO:0051085 IDA
    protein-containing complex assembly GO:0065003 IDA
    cellular response to misfolded protein GO:0071218 IBA
    cellular response to misfolded protein GO:0071218 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    membrane GO:0016020 HDA
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified DNAJB12 in sEVs
1
Experiment ID 834
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DNAJB12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGTA 6449
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
2 UGGT2 55757
Proximity Label-MS Homo sapiens
3 TUSC2  
Affinity Capture-MS Homo sapiens
4 STT3A 3703
Proximity Label-MS Homo sapiens
5 MME 4311
Affinity Capture-MS Homo sapiens
6 HSPA1L 3305
Affinity Capture-MS Homo sapiens
7 C9orf78 51759
Affinity Capture-MS Homo sapiens
8 EYA2  
Affinity Capture-MS Homo sapiens
9 GRAMD1A  
Proximity Label-MS Homo sapiens
10 APP 351
Proximity Label-MS Homo sapiens
11 DNAJB6 10049
Affinity Capture-MS Homo sapiens
12 CAPZB 832
Affinity Capture-MS Homo sapiens
13 PLOD3 8985
Proximity Label-MS Homo sapiens
14 RNF5  
Affinity Capture-Western Homo sapiens
15 HSPA2 3306
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
16 RCN2 5955
Proximity Label-MS Homo sapiens
17 NUP210 23225
Proximity Label-MS Homo sapiens
18 DDX58 23586
Affinity Capture-RNA Homo sapiens
19 DDOST 1650
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
20 DNAJB1 3337
Affinity Capture-MS Homo sapiens
21 STIP1 10963
Proximity Label-MS Homo sapiens
22 PDIA6 10130
Proximity Label-MS Homo sapiens
23 CCDC47 57003
Affinity Capture-MS Homo sapiens
24 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CALR 811
Proximity Label-MS Homo sapiens
26 KIF14 9928
Affinity Capture-MS Homo sapiens
27 HSPA8 3312
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
28 EMC1 23065
Proximity Label-MS Homo sapiens
29 LMAN1 3998
Proximity Label-MS Homo sapiens
30 E2F4  
Affinity Capture-MS Homo sapiens
31 LRRC59 55379
Proximity Label-MS Homo sapiens
32 PIGH  
Affinity Capture-MS Homo sapiens
33 PPIB 5479
Proximity Label-MS Homo sapiens
34 DNAJB8 165721
Affinity Capture-MS Homo sapiens
35 EMD 2010
Affinity Capture-MS Homo sapiens
36 CD74 972
Affinity Capture-MS Homo sapiens
37 HSP90B1 7184
Proximity Label-MS Homo sapiens
38 BAG6 7917
Proximity Label-MS Homo sapiens
39 CLCC1 23155
Proximity Label-MS Homo sapiens
40 BTF3 689
Affinity Capture-MS Homo sapiens
41 GPR182  
Affinity Capture-MS Homo sapiens
42 DNAJA1 3301
Affinity Capture-MS Homo sapiens
43 P4HB 5034
Proximity Label-MS Homo sapiens
44 RPN1 6184
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
45 GANAB 23193
Proximity Label-MS Homo sapiens
46 SDF4 51150
Proximity Label-MS Homo sapiens
47 DNAJB5  
Affinity Capture-MS Homo sapiens
48 PTPRO 5800
Affinity Capture-MS Homo sapiens
49 SEC61B 10952
Proximity Label-MS Homo sapiens
50 DNAJB11 51726
Affinity Capture-MS Homo sapiens
51 RER1 11079
Affinity Capture-MS Homo sapiens
52 ST13 6767
Proximity Label-MS Homo sapiens
53 NUCB2 4925
Proximity Label-MS Homo sapiens
54 DNAJB14  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
55 HSPA5 3309
Affinity Capture-MS Homo sapiens
56 CNGA4  
Affinity Capture-MS Homo sapiens
57 RPN2 6185
Affinity Capture-MS Homo sapiens
58 CANX 821
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
59 SELT 51714
Proximity Label-MS Homo sapiens
60 AMBRA1  
Affinity Capture-MS Homo sapiens
61 CALR3  
Proximity Label-MS Homo sapiens
62 BAG2 9532
Affinity Capture-MS Homo sapiens
63 BCAP31 10134
Proximity Label-MS Homo sapiens
64 CUL3 8452
Affinity Capture-MS Homo sapiens
65 KLHL29  
Affinity Capture-MS Homo sapiens
66 PDIA3 2923
Proximity Label-MS Homo sapiens
67 EGFR 1956
Affinity Capture-MS Homo sapiens
68 RIPK4  
Affinity Capture-MS Homo sapiens
69 RCN1 5954
Proximity Label-MS Homo sapiens
70 ABHD12 26090
Proximity Label-MS Homo sapiens
71 MIA3 375056
Proximity Label-MS Homo sapiens
72 UGGT1 56886
Proximity Label-MS Homo sapiens
73 MAPK6  
Affinity Capture-MS Homo sapiens
74 YME1L1 10730
Affinity Capture-MS Homo sapiens
75 MYC  
Affinity Capture-MS Homo sapiens
76 HSPA1A 3303
Affinity Capture-MS Homo sapiens
77 DSTN 11034
Co-fractionation Homo sapiens
78 DNAJC3 5611
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 HYOU1 10525
Proximity Label-MS Homo sapiens
80 SLC12A6 9990
Affinity Capture-MS Homo sapiens
81 HSPA9 3313
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
82 LMNA 4000
Proximity Label-MS Homo sapiens
83 MAGT1 84061
Proximity Label-MS Homo sapiens
84 BAG4  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
85 OST4  
Affinity Capture-MS Homo sapiens
86 STUB1 10273
Affinity Capture-MS Homo sapiens
87 LAMB1 3912
Proximity Label-MS Homo sapiens
88 SUN1 23353
Proximity Label-MS Homo sapiens
89 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
90 PRKCSH 5589
Proximity Label-MS Homo sapiens
91 TMED10 10972
Affinity Capture-MS Homo sapiens
92 NAAA  
Affinity Capture-MS Homo sapiens
93 TTC1 7265
Affinity Capture-MS Homo sapiens
94 PDIA4 9601
Proximity Label-MS Homo sapiens
95 CALU 813
Proximity Label-MS Homo sapiens
96 M6PR 4074
Affinity Capture-MS Homo sapiens
97 EDEM1  
Affinity Capture-MS Homo sapiens
98 GRPEL1 80273
Affinity Capture-MS Homo sapiens
99 PLOD1 5351
Proximity Label-MS Homo sapiens
100 C21orf33  
Proximity Label-MS Homo sapiens
101 TUBB6 84617
Affinity Capture-MS Homo sapiens
102 MARCH4  
Affinity Capture-MS Homo sapiens
103 BAG5 9529
Affinity Capture-MS Homo sapiens
104 LRPAP1 4043
Proximity Label-MS Homo sapiens
105 EXT2 2132
Proximity Label-MS Homo sapiens
106 CDK9 1025
Affinity Capture-MS Homo sapiens
107 CABYR  
Affinity Capture-MS Homo sapiens
108 STT3B 201595
Proximity Label-MS Homo sapiens
109 CLPTM1L 81037
Proximity Label-MS Homo sapiens
110 COLGALT1 79709
Proximity Label-MS Homo sapiens
111 C9orf72  
Affinity Capture-MS Homo sapiens
112 DIAPH1 1729
Affinity Capture-MS Homo sapiens
113 CKAP4 10970
Proximity Label-MS Homo sapiens
114 DERL1 79139
Proximity Label-MS Homo sapiens
115 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNAJB12 is involved
No pathways found





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