Gene description for NUP210
Gene name nucleoporin 210kDa
Gene symbol NUP210
Other names/aliases GP210
POM210
Species Homo sapiens
 Database cross references - NUP210
ExoCarta ExoCarta_23225
Vesiclepedia VP_23225
Entrez Gene 23225
HGNC 30052
MIM 607703
UniProt Q8TEM1  
 NUP210 identified in sEVs derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for NUP210
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    nucleocytoplasmic transport GO:0006913 NAS
    protein transport GO:0015031 IEA
    mRNA transport GO:0051028 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 TAS
    nuclear pore GO:0005643 IBA
    nuclear pore GO:0005643 NAS
    endoplasmic reticulum membrane GO:0005789 IEA
    membrane GO:0016020 HDA
    nuclear membrane GO:0031965 IEA
 Experiment description of studies that identified NUP210 in sEVs
1
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NUP210
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLFN11 91607
Affinity Capture-MS Homo sapiens
2 LGR4 55366
Affinity Capture-MS Homo sapiens
3 NAPA 8775
Affinity Capture-MS Homo sapiens
4 NFATC1 4772
Affinity Capture-MS Homo sapiens
5 ADRB2  
Affinity Capture-MS Homo sapiens
6 NOP56 10528
Proximity Label-MS Homo sapiens
7 HSPA13 6782
Proximity Label-MS Homo sapiens
8 PLD4  
Affinity Capture-MS Homo sapiens
9 DNAJC25 548645
Proximity Label-MS Homo sapiens
10 LRRC59 55379
Proximity Label-MS Homo sapiens
11 HSPA5 3309
Proximity Label-MS Homo sapiens
12 KIAA1429 25962
Affinity Capture-MS Homo sapiens
13 DNAJC10 54431
Proximity Label-MS Homo sapiens
14 ULK3 25989
Affinity Capture-MS Homo sapiens
15 NUP153 9972
Affinity Capture-MS Homo sapiens
16 HARS2 23438
Affinity Capture-MS Homo sapiens
17 PRC1 9055
Affinity Capture-MS Homo sapiens
18 ETFA 2108
Co-fractionation Homo sapiens
19 OBSL1 23363
Affinity Capture-MS Homo sapiens
20 DDOST 1650
Affinity Capture-MS Homo sapiens
21 RPN2 6185
Proximity Label-MS Homo sapiens
22 ITGA4 3676
Affinity Capture-MS Homo sapiens
23 EED  
Affinity Capture-MS Homo sapiens
24 ATG16L1 55054
Affinity Capture-MS Homo sapiens
25 Nup98  
Affinity Capture-MS Mus musculus
26 SMYD4  
Affinity Capture-MS Homo sapiens
27 SARS 6301
Co-fractionation Homo sapiens
28 NUP155 9631
Proximity Label-MS Homo sapiens
29 CHMP4C 92421
Affinity Capture-MS Homo sapiens
30 XPO1 7514
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 Rcc1  
Affinity Capture-MS Mus musculus
32 NONO 4841
Co-fractionation Homo sapiens
33 MAPRE1 22919
Affinity Capture-MS Homo sapiens
34 UBE2I 7329
Affinity Capture-MS Homo sapiens
35 RAN 5901
Affinity Capture-MS Homo sapiens
36 NTRK1 4914
Affinity Capture-MS Homo sapiens
37 DNAJB9 4189
Proximity Label-MS Homo sapiens
38 FLNB 2317
Co-fractionation Homo sapiens
39 KPNB1 3837
Affinity Capture-MS Homo sapiens
40 RPN1 6184
Proximity Label-MS Homo sapiens
41 DNAJC30  
Proximity Label-MS Homo sapiens
42 SEC61B 10952
Proximity Label-MS Homo sapiens
43 DNAJB11 51726
Proximity Label-MS Homo sapiens
44 COX4I1 1327
Proximity Label-MS Homo sapiens
45 NUMA1 4926
Affinity Capture-MS Homo sapiens
46 DNAJB14  
Proximity Label-MS Homo sapiens
47 SFPQ 6421
Co-fractionation Homo sapiens
48 FANCD2  
Affinity Capture-MS Homo sapiens
49 METTL14  
Affinity Capture-MS Homo sapiens
50 DNAJB12 54788
Proximity Label-MS Homo sapiens
51 CALR3  
Proximity Label-MS Homo sapiens
52 ECT2 1894
Affinity Capture-MS Homo sapiens
53 SIRT7  
Affinity Capture-MS Homo sapiens
54 CUL3 8452
Affinity Capture-MS Homo sapiens
55 NR3C1 2908
Proximity Label-MS Homo sapiens
56 EXOSC5 56915
Two-hybrid Homo sapiens
57 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 NUP214 8021
Affinity Capture-MS Homo sapiens
59 FN1 2335
Affinity Capture-MS Homo sapiens
60 DNAJC18  
Proximity Label-MS Homo sapiens
61 Nup107  
Affinity Capture-MS Mus musculus
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 ATP2A1 487
Proximity Label-MS Homo sapiens
64 FBXO2 26232
Affinity Capture-MS Homo sapiens
65 LACTB2 51110
Co-fractionation Homo sapiens
66 PTPRN  
Proximity Label-MS Homo sapiens
67 VRK2 7444
Affinity Capture-MS Homo sapiens
68 DNAJC3 5611
Proximity Label-MS Homo sapiens
69 RANBP1 5902
Affinity Capture-MS Homo sapiens
70 SEH1L 81929
Affinity Capture-MS Homo sapiens
71 CANX 821
Affinity Capture-MS Homo sapiens
72 AKAP1 8165
Proximity Label-MS Homo sapiens
73 HIF1A 3091
Affinity Capture-MS Homo sapiens
74 TRIP6 7205
Two-hybrid Homo sapiens
75 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
76 SIRT6  
Affinity Capture-MS Homo sapiens
77 RHOBTB3 22836
Affinity Capture-MS Homo sapiens
78 PDIA4 9601
Proximity Label-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
79 CALU 813
Proximity Label-MS Homo sapiens
80 MEN1 4221
Affinity Capture-MS Homo sapiens
81 NUDCD2 134492
Co-fractionation Homo sapiens
82 NUP35 129401
Proximity Label-MS Homo sapiens
83 OS9 10956
Affinity Capture-MS Homo sapiens
84 Nup214  
Affinity Capture-MS Mus musculus
85 ERGIC2 51290
Proximity Label-MS Homo sapiens
86 NUP107 57122
Affinity Capture-MS Homo sapiens
87 E2F4  
Affinity Capture-MS Homo sapiens
88 PINK1  
Affinity Capture-MS Homo sapiens
89 KYNU  
Co-fractionation Homo sapiens
90 CUL7 9820
Affinity Capture-MS Homo sapiens
91 C9orf72  
Affinity Capture-MS Homo sapiens
92 CCRN4L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NUP210 is involved
PathwayEvidenceSource
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Disorders of transmembrane transporters TAS Reactome
Export of Viral Ribonucleoproteins from Nucleus TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
HCMV Late Events TAS Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle TAS Reactome
Host Interactions of HIV factors IEA Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Interactions of Rev with host cellular proteins IEA Reactome
Interactions of Rev with host cellular proteins TAS Reactome
Interactions of Vpr with host cellular proteins TAS Reactome
Interferon Signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome
Late Phase of HIV Life Cycle TAS Reactome
M Phase TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of non-coding RNA TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Mitotic Prophase TAS Reactome
NEP/NS2 Interacts with the Cellular Export Machinery TAS Reactome
NS1 Mediated Effects on Host Pathways TAS Reactome
Nuclear Envelope Breakdown TAS Reactome
Nuclear import of Rev protein IEA Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Rev-mediated nuclear export of HIV RNA TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
SLC transporter disorders TAS Reactome
snRNP Assembly TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation TAS Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of DNA replication proteins TAS Reactome
SUMOylation of RNA binding proteins TAS Reactome
SUMOylation of SUMOylation proteins TAS Reactome
SUMOylation of ubiquitinylation proteins TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript TAS Reactome
Transport of Mature mRNA Derived from an Intronless Transcript TAS Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts TAS Reactome
Transport of Mature Transcript to Cytoplasm TAS Reactome
Transport of Ribonucleoproteins into the Host Nucleus TAS Reactome
Transport of the SLBP Dependant Mature mRNA TAS Reactome
Transport of the SLBP independent Mature mRNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Messenger RNA Synthesis TAS Reactome
Vpr-mediated nuclear import of PICs TAS Reactome





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