Gene description for Tardbp
Gene name TAR DNA binding protein
Gene symbol Tardbp
Other names/aliases 1190002A23Rik
C85084
TDP-43
Tdp43
Species Mus musculus
 Database cross references - Tardbp
ExoCarta ExoCarta_230908
Vesiclepedia VP_230908
Entrez Gene 230908
UniProt Q921F2  
 Tardbp identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tardbp
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA binding GO:0003677 ISO
    double-stranded DNA binding GO:0003690 ISO
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    RNA binding GO:0003723 ISO
    mRNA 3'-UTR binding GO:0003730 IEA
    mRNA 3'-UTR binding GO:0003730 ISO
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    pre-mRNA intronic binding GO:0097157 IDA
    molecular condensate scaffold activity GO:0140693 IEA
    molecular condensate scaffold activity GO:0140693 ISO
Biological Process
    negative regulation of protein phosphorylation GO:0001933 ISO
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IMP
    RNA splicing GO:0008380 ISO
    gene expression GO:0010467 IMP
    regulation of gene expression GO:0010468 IBA
    negative regulation of gene expression GO:0010629 ISO
    regulation of protein stability GO:0031647 IMP
    regulation of protein stability GO:0031647 ISO
    positive regulation of insulin secretion GO:0032024 IEA
    positive regulation of insulin secretion GO:0032024 ISO
    response to endoplasmic reticulum stress GO:0034976 IEA
    positive regulation of protein import into nucleus GO:0042307 IDA
    regulation of circadian rhythm GO:0042752 IMP
    regulation of apoptotic process GO:0042981 IEA
    regulation of apoptotic process GO:0042981 ISO
    negative regulation by host of viral transcription GO:0043922 IEA
    negative regulation by host of viral transcription GO:0043922 ISO
    rhythmic process GO:0048511 IEA
    regulation of cell cycle GO:0051726 IEA
    regulation of cell cycle GO:0051726 ISO
    3'-UTR-mediated mRNA destabilization GO:0061158 IEA
    3'-UTR-mediated mRNA destabilization GO:0061158 ISO
    3'-UTR-mediated mRNA stabilization GO:0070935 IEA
    3'-UTR-mediated mRNA stabilization GO:0070935 ISO
    nuclear inner membrane organization GO:0071765 IEA
    nuclear inner membrane organization GO:0071765 ISO
    amyloid fibril formation GO:1990000 IEA
    amyloid fibril formation GO:1990000 ISO
Subcellular Localization
    chromatin GO:0000785 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 ISO
    perichromatin fibrils GO:0005726 IEA
    perichromatin fibrils GO:0005726 ISO
    mitochondrion GO:0005739 IEA
    cytoplasmic stress granule GO:0010494 IEA
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    interchromatin granule GO:0035061 IEA
    interchromatin granule GO:0035061 ISO
    intracellular membraneless organelle GO:0043232 ISO
 Experiment description of studies that identified Tardbp in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tardbp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ddx5 13207
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
2 Zfand2b  
Co-fractionation Mus musculus
3 Snrnp200 320632
Co-fractionation Mus musculus
4 Upf1 19704
Co-fractionation Mus musculus
5 Ruvbl2 20174
Co-fractionation Mus musculus
6 Rpl13a 22121
Co-fractionation Mus musculus
7 Hnrnpul2 68693
Co-fractionation Mus musculus
8 Ewsr1 14030
Co-fractionation Mus musculus
9 Celf1  
Two-hybrid Mus musculus
10 Gtf2e2  
Two-hybrid Mus musculus
11 Mettl21e  
Affinity Capture-MS Mus musculus
12 PTBP1 5725
Two-hybrid Homo sapiens
13 Ldb1  
Two-hybrid Mus musculus
14 Rbm39 170791
Co-fractionation Mus musculus
15 Mtdh 67154
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
16 Tfe3  
Affinity Capture-MS Mus musculus
17 Hnrnpu 51810
Co-fractionation Mus musculus
18 Tia1  
Affinity Capture-Western Mus musculus
19 Elavl2  
Co-fractionation Mus musculus
20 Ddx17 67040
Co-fractionation Mus musculus
21 Mecp2  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
22 Tial1  
Co-fractionation Mus musculus
23 Hnrnph1 59013
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
24 Tardbp 230908
Two-hybrid Mus musculus
Two-hybrid Mus musculus
25 Ddx3x 13205
Co-fractionation Mus musculus
26 Zfr  
Co-fractionation Mus musculus
27 Hnrnpa3 229279
Co-fractionation Mus musculus
28 Rpl9 20005
Co-fractionation Mus musculus
29 Dhx36  
Co-fractionation Mus musculus
30 RBBP5 5929
Affinity Capture-MS Homo sapiens
31 Fam98a  
Co-fractionation Mus musculus
32 Pabpn1 54196
Co-fractionation Mus musculus
33 Ddx1 104721
Co-fractionation Mus musculus
34 Dhx15 13204
Co-fractionation Mus musculus
35 Rps17 20068
Co-fractionation Mus musculus
36 Eed  
Affinity Capture-MS Mus musculus
37 Nacc1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
38 Gtf2e1  
Two-hybrid Mus musculus
39 Ube2i  
Two-hybrid Mus musculus
40 Rpl3 27367
Co-fractionation Mus musculus
41 Atg16l1  
Affinity Capture-MS Mus musculus
42 Msi2  
Co-fractionation Mus musculus
43 Srrt 83701
Co-fractionation Mus musculus
44 Med16  
Two-hybrid Mus musculus
45 Fancd2  
Affinity Capture-MS Mus musculus
46 Fus  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Tardbp is involved
No pathways found





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