Gene description for Ddx1
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Gene symbol Ddx1
Other names/aliases AA409185
DBP-RB
Species Mus musculus
 Database cross references - Ddx1
ExoCarta ExoCarta_104721
Vesiclepedia VP_104721
Entrez Gene 104721
UniProt Q91VR5  
 Ddx1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ddx1
Molecular Function
    DNA binding GO:0003677 IEA
    chromatin binding GO:0003682 ISO
    chromatin binding GO:0003682 ISS
    DNA clamp loader activity GO:0003689 IEA
    transcription coregulator activity GO:0003712 ISO
    transcription coregulator activity GO:0003712 ISS
    RNA helicase activity GO:0003724 ISO
    RNA helicase activity GO:0003724 ISS
    double-stranded RNA binding GO:0003725 IDA
    nuclease activity GO:0004518 ISO
    nuclease activity GO:0004518 ISS
    exonuclease activity GO:0004527 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    poly(A) binding GO:0008143 ISO
    poly(A) binding GO:0008143 ISS
    ATP hydrolysis activity GO:0016887 IEA
    DNA/RNA helicase activity GO:0033677 ISO
    DNA/RNA helicase activity GO:0033677 ISS
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    positive regulation of myeloid dendritic cell cytokine production GO:0002735 IMP
    double-strand break repair GO:0006302 ISO
    double-strand break repair GO:0006302 ISS
    chromatin remodeling GO:0006338 IEA
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 ISO
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 ISS
    mRNA processing GO:0006397 IEA
    response to virus GO:0009615 IMP
    DNA duplex unwinding GO:0032508 ISO
    DNA duplex unwinding GO:0032508 ISS
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    response to exogenous dsRNA GO:0043330 IMP
    innate immune response GO:0045087 IEA
    defense response to virus GO:0051607 IEA
    nucleic acid metabolic process GO:0090304 ISO
    chromatin looping GO:0140588 IEA
    protein localization to cytoplasmic stress granule GO:1903608 IEA
    protein localization to cytoplasmic stress granule GO:1903608 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytoplasmic stress granule GO:0010494 IDA
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    cleavage body GO:0071920 ISO
    cleavage body GO:0071920 ISS
    tRNA-splicing ligase complex GO:0072669 ISO
    tRNA-splicing ligase complex GO:0072669 ISS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Ddx1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ddx1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Npm1 18148
Affinity Capture-MS Mus musculus
2 Cebpa  
Affinity Capture-MS Mus musculus
3 Stau1  
Co-fractionation Mus musculus
4 2700060E02Rik 68045
Co-fractionation Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Tardbp 230908
Co-fractionation Mus musculus
7 Eed  
Affinity Capture-MS Mus musculus
8 MKRN1 23608
Affinity Capture-MS Homo sapiens
9 Larp1  
Co-fractionation Mus musculus
10 Atg16l1  
Affinity Capture-MS Mus musculus
11 Gigyf2  
Co-fractionation Mus musculus
12 Lsm14a  
Co-fractionation Mus musculus
13 Nanog  
Affinity Capture-MS Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
15 Kctd13  
Affinity Capture-MS Mus musculus
16 Rtcb 28088
Co-fractionation Mus musculus
17 Hnrnpa2b1 53379
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Ddx1 is involved
No pathways found





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