Gene description for Rtcb
Gene name RNA 2',3'-cyclic phosphate and 5'-OH ligase
Gene symbol Rtcb
Other names/aliases AI255213
AI463255
D10Wsu52e
FAAP
HSPC117
Species Mus musculus
 Database cross references - Rtcb
ExoCarta ExoCarta_28088
Vesiclepedia VP_28088
Entrez Gene 28088
UniProt Q99LF4  
 Rtcb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rtcb
Molecular Function
    RNA ligase (ATP) activity GO:0003972 ISO
    GTP binding GO:0005525 IEA
    vinculin binding GO:0017166 IEA
    vinculin binding GO:0017166 ISO
    metal ion binding GO:0046872 IEA
    RNA ligase (GTP) activity GO:0170057 ISS
Biological Process
    in utero embryonic development GO:0001701 IMP
    placenta development GO:0001890 IMP
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 IBA
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 ISO
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nuclear envelope GO:0005635 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum membrane GO:0005789 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    tRNA-splicing ligase complex GO:0072669 IBA
    tRNA-splicing ligase complex GO:0072669 ISO
    tRNA-splicing ligase complex GO:0072669 ISS
 Experiment description of studies that identified Rtcb in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rtcb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ddx1 104721
Co-fractionation Mus musculus
2 U2af2 22185
Two-hybrid Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Mettl21e  
Affinity Capture-MS Mus musculus
5 2700060E02Rik 68045
Co-fractionation Mus musculus
6 Atxn1  
Proximity Label-MS Mus musculus
7 Fam98b 68215
Co-fractionation Mus musculus
8 Eed  
Affinity Capture-MS Mus musculus
9 Nmt1  
Co-fractionation Mus musculus
10 Polr1c  
Co-fractionation Mus musculus
11 Krt18 16668
Co-fractionation Mus musculus
12 Trmt10c  
Co-fractionation Mus musculus
13 Nanog  
Affinity Capture-MS Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
15 Rars2  
Co-fractionation Mus musculus
16 Pef1  
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Rtcb is involved
No pathways found





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