Gene description for Snrnp200
Gene name small nuclear ribonucleoprotein 200 (U5)
Gene symbol Snrnp200
Other names/aliases A330064G03Rik
Ascc3l1
BC011390
HELIC2
U5-200-KD
U5-200KD
Species Mus musculus
 Database cross references - Snrnp200
ExoCarta ExoCarta_320632
Vesiclepedia VP_320632
Entrez Gene 320632
UniProt Q6P4T2  
 Snrnp200 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Snrnp200
Molecular Function
    nucleic acid binding GO:0003676 IEA
    DNA clamp loader activity GO:0003689 IEA
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 ISO
    RNA helicase activity GO:0003724 ISS
    helicase activity GO:0004386 ISO
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) GO:0000388 IBA
    spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) GO:0000388 IEA
    spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) GO:0000388 ISO
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    chromatin remodeling GO:0006338 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 IBA
    spliceosomal complex GO:0005681 ISO
    U5 snRNP GO:0005682 IEA
    U5 snRNP GO:0005682 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 IEA
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    U2-type catalytic step 1 spliceosome GO:0071006 ISO
    U2-type catalytic step 1 spliceosome GO:0071006 ISS
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Snrnp200 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Snrnp200
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Ddx5 13207
Co-fractionation Mus musculus
3 Ddx39b 53817
Co-fractionation Mus musculus
4 Sf3b3 101943
Co-fractionation Mus musculus
5 Prpf3 70767
Co-fractionation Mus musculus
6 Hnrnpc 15381
Co-fractionation Mus musculus
7 Slu7  
Co-fractionation Mus musculus
8 Zmat2  
Co-fractionation Mus musculus
9 Prpf19 28000
Co-fractionation Mus musculus
10 Snap23 20619
Co-fractionation Mus musculus
11 Cdc5l 71702
Co-fractionation Mus musculus
12 Sart3  
Co-fractionation Mus musculus
13 Snw1  
Co-fractionation Mus musculus
14 Hnrnpm 76936
Co-fractionation Mus musculus
15 Atxn1  
Affinity Capture-MS Mus musculus
16 Rbm39 170791
Co-fractionation Mus musculus
17 Kctd13  
Affinity Capture-MS Mus musculus
18 Snrpd2 107686
Co-fractionation Mus musculus
19 Frg1  
Co-fractionation Mus musculus
20 Pikfyve  
Co-fractionation Mus musculus
21 Cwc22  
Co-fractionation Mus musculus
22 Chaf1a  
Affinity Capture-MS Mus musculus
23 Prpf4  
Co-fractionation Mus musculus
24 Snrpd3 67332
Co-fractionation Mus musculus
25 Crnkl1  
Co-fractionation Mus musculus
26 Chchd1  
Co-fractionation Mus musculus
27 Mov10 17454
Co-fractionation Mus musculus
28 Mettl21e  
Affinity Capture-MS Mus musculus
29 Hnrnph1 59013
Co-fractionation Mus musculus
30 Tardbp 230908
Co-fractionation Mus musculus
31 Pabpc1 18458
Co-fractionation Mus musculus
32 Hnrnpu 51810
Co-fractionation Mus musculus
33 Ywhab 54401
Co-fractionation Mus musculus
34 Eftud2 20624
Co-fractionation Mus musculus
35 Srsf2 20382
Co-fractionation Mus musculus
36 2310036O22Rik  
Co-fractionation Mus musculus
37 Ncbp2  
Co-fractionation Mus musculus
38 Plrg1  
Co-fractionation Mus musculus
39 Ctnnbl1  
Co-fractionation Mus musculus
40 Srrm2  
Co-fractionation Mus musculus
41 Htatsf1  
Co-fractionation Mus musculus
42 Sfpq 71514
Co-fractionation Mus musculus
43 Dhx15 13204
Co-fractionation Mus musculus
44 Hnrnpa2b1 53379
Co-fractionation Mus musculus
45 Hnrnpf 98758
Co-fractionation Mus musculus
46 Skiv2l2  
Co-fractionation Mus musculus
47 Hnrnpk 15387
Co-fractionation Mus musculus
48 Ppil3  
Co-fractionation Mus musculus
49 Rbm8a  
Co-fractionation Mus musculus
50 Syncrip 56403
Co-fractionation Mus musculus
51 Cwc27  
Co-fractionation Mus musculus
52 U2af2 22185
Co-fractionation Mus musculus
53 Sf1 22668
Co-fractionation Mus musculus
54 Ppp2r5c 26931
Co-fractionation Mus musculus
55 Eif4a3 192170
Co-fractionation Mus musculus
56 Prpf31  
Co-fractionation Mus musculus
57 Smndc1  
Co-fractionation Mus musculus
58 Txnl4a  
Co-fractionation Mus musculus
59 Fancd2  
Affinity Capture-MS Mus musculus
60 Tcea1 21399
Co-fractionation Mus musculus
61 Paxbp1  
Co-fractionation Mus musculus
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