Gene description for Sf1
Gene name splicing factor 1
Gene symbol Sf1
Other names/aliases BBP
MZFM
WBP4
Zfp162
Species Mus musculus
 Database cross references - Sf1
ExoCarta ExoCarta_22668
Vesiclepedia VP_22668
Entrez Gene 22668
 Sf1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Sf1
Molecular Function
    RNA binding GO:0003723 IPI
    mRNA binding GO:0003729 IBA
    zinc ion binding GO:0008270 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    mRNA splicing, via spliceosome GO:0000398 NAS
    male sex determination GO:0030238 IGI
    nuclear body organization GO:0030575 IMP
    Leydig cell differentiation GO:0033327 IGI
    regulation of mRNA splicing, via spliceosome GO:0048024 IBA
    negative regulation of smooth muscle cell proliferation GO:0048662 IEA
    negative regulation of smooth muscle cell proliferation GO:0048662 ISO
    regulation of steroid biosynthetic process GO:0050810 IGI
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 ISO
    cytoplasm GO:0005737 ISS
    nuclear body GO:0016604 IDA
    U2AF complex GO:0089701 ISO
 Experiment description of studies that identified Sf1 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Sf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pin1  
Affinity Capture-Western Mus musculus
2 Snw1  
Co-fractionation Mus musculus
3 Whsc1  
Affinity Capture-MS Mus musculus
4 Egr1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
5 Kcna3  
Affinity Capture-MS Mus musculus
6 Ctnnbl1  
Co-fractionation Mus musculus
7 U2af2 22185
Co-fractionation Mus musculus
8 Snrnp200 320632
Co-fractionation Mus musculus
9 Hnrnpc 15381
Co-fractionation Mus musculus
10 Pitx1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
11 Hnrnpa2b1 53379
Co-fractionation Mus musculus
12 Ddx39b 53817
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Sf1 is involved
No pathways found





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