Gene description for Ywhab
Gene name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
Gene symbol Ywhab
Other names/aliases 1300003C17Rik
Species Mus musculus
 Database cross references - Ywhab
ExoCarta ExoCarta_54401
Vesiclepedia VP_54401
Entrez Gene 54401
UniProt Q9CQV8  
 Ywhab identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Mast cells 17486113    
Microglia 16081791    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ywhab
Molecular Function
    protein kinase inhibitor activity GO:0004860 ISO
    protein kinase inhibitor activity GO:0004860 ISS
    protein phosphatase inhibitor activity GO:0004864 IEA
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 ISO
    protein domain specific binding GO:0019904 IDA
    protein domain specific binding GO:0019904 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    histone deacetylase binding GO:0042826 IEA
    histone deacetylase binding GO:0042826 ISO
    protein-containing complex binding GO:0044877 ISO
    phosphoserine residue binding GO:0050815 IEA
    phosphoserine residue binding GO:0050815 ISO
    phosphoprotein binding GO:0051219 ISO
Biological Process
    protein targeting GO:0006605 IDA
    signal transduction GO:0007165 IBA
    protein localization GO:0008104 IBA
    positive regulation of catalytic activity GO:0043085 ISO
    negative regulation of G protein-coupled receptor signaling pathway GO:0045744 ISO
    negative regulation of G protein-coupled receptor signaling pathway GO:0045744 ISS
    negative regulation of DNA-templated transcription GO:0045892 ISO
    cytoplasmic sequestering of protein GO:0051220 IEA
    cytoplasmic sequestering of protein GO:0051220 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    transcription repressor complex GO:0017053 ISO
    protein-containing complex GO:0032991 ISO
    melanosome GO:0042470 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Ywhab in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
6
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ywhab
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pfkfb2  
Co-fractionation Mus musculus
2 Rmdn3  
Co-fractionation Mus musculus
3 TNFAIP3 7128
Two-hybrid Homo sapiens
4 Ywhah 22629
Co-fractionation Mus musculus
5 Hsp90ab1 15516
Co-fractionation Mus musculus
6 Sipa1l3  
Co-fractionation Mus musculus
7 Rin1  
Two-hybrid Mus musculus
8 G6pdx 14381
Co-fractionation Mus musculus
9 Pi4kb  
Co-fractionation Mus musculus
10 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
13 Itgb2 16414
Affinity Capture-Western Mus musculus
14 Ncam1 17967
Affinity Capture-Western Mus musculus
15 Lsm2  
Co-fractionation Mus musculus
16 Atxn1  
Proximity Label-MS Mus musculus
17 Slc9a1  
Co-fractionation Mus musculus
18 MTNR1A  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
19 Mark3  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
20 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
21 Tfe3  
Affinity Capture-MS Mus musculus
22 Snrnp200 320632
Co-fractionation Mus musculus
23 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
24 Bcl2l11  
Affinity Capture-Western Mus musculus
25 Lrrk2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
26 Kcnma1  
Affinity Capture-MS Mus musculus
27 Ywhaz 22631
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
28 Zfp36  
Affinity Capture-Western Mus musculus
29 Emd  
Co-fractionation Mus musculus
30 Ywhaq 22630
Co-fractionation Mus musculus
31 Akt1s1  
Co-fractionation Mus musculus
32 Hsp90aa1 15519
Co-fractionation Mus musculus
33 Slc8a2  
Co-fractionation Mus musculus
34 Pfdn2  
Co-fractionation Mus musculus
35 MAPT  
Reconstituted Complex Homo sapiens
36 Atg5  
Affinity Capture-RNA Mus musculus
37 Vipas39  
Co-fractionation Mus musculus
38 Cdc37 12539
Co-fractionation Mus musculus
39 Unk  
Affinity Capture-RNA Mus musculus
40 Actb 11461
Co-fractionation Mus musculus
41 Nedd4l 83814
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
42 Dyrk1a  
Co-fractionation Mus musculus
43 Pik3c2a  
Co-fractionation Mus musculus
44 Vps33b  
Co-fractionation Mus musculus
45 Crk 12928
Affinity Capture-MS Mus musculus
46 Lnx1  
Affinity Capture-MS Mus musculus
47 Hspa4 15525
Co-fractionation Mus musculus
48 Arhgef7  
Affinity Capture-Western Mus musculus
49 Hnrnpk 15387
Co-fractionation Mus musculus
50 Nedd4 17999
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
51 Eed  
Affinity Capture-MS Mus musculus
52 Ywhae 22627
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
53 Mapt  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
54 Atg16l1  
Affinity Capture-MS Mus musculus
55 Sptan1 20740
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
56 Hdac4  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
57 Cep170b  
Co-fractionation Mus musculus
58 Ywhag 22628
Co-fractionation Mus musculus
59 Fancd2  
Affinity Capture-MS Mus musculus
60 Homer1  
Affinity Capture-MS Mus musculus
61 G3bp1  
Co-fractionation Mus musculus
62 Mark2 13728
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Ywhab is involved
PathwayEvidenceSource
Activation of BAD and translocation to mitochondria IEA Reactome
Activation of BH3-only proteins IEA Reactome
Adaptive Immune System IEA Reactome
Apoptosis IEA Reactome
Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex IEA Reactome
FOXO-mediated transcription IEA Reactome
Frs2-mediated activation IEA Reactome
G2/M Checkpoints IEA Reactome
G2/M DNA damage checkpoint IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Immune System IEA Reactome
Intrinsic Pathway for Apoptosis IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Metabolism of RNA IEA Reactome
MTOR signalling IEA Reactome
mTORC1-mediated signalling IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Programmed Cell Death IEA Reactome
Prolonged ERK activation events IEA Reactome
RAF activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Rap1 signalling IEA Reactome
Regulation of localization of FOXO transcription factors IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases activate PKNs IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hippo IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signalling to ERKs IEA Reactome
TP53 Regulates Metabolic Genes IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA IEA Reactome





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