Gene description for Mark2
Gene name MAP/microtubule affinity-regulating kinase 2
Gene symbol Mark2
Other names/aliases EMK-1
Emk
Par-1
Par-1b
Species Mus musculus
 Database cross references - Mark2
ExoCarta ExoCarta_13728
Vesiclepedia VP_13728
Entrez Gene 13728
UniProt Q05512  
 Mark2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mark2
Molecular Function
    magnesium ion binding GO:0000287 ISO
    magnesium ion binding GO:0000287 ISS
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    lipid binding GO:0008289 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    tau protein binding GO:0048156 ISO
    tau-protein kinase activity GO:0050321 IBA
    tau-protein kinase activity GO:0050321 ISO
    tau-protein kinase activity GO:0050321 ISS
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    molecular function activator activity GO:0140677 ISO
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    neuron migration GO:0001764 ISO
    neuron migration GO:0001764 ISS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    positive regulation of neuron projection development GO:0010976 ISO
    positive regulation of neuron projection development GO:0010976 ISS
    Wnt signaling pathway GO:0016055 IEA
    peptidyl-serine phosphorylation GO:0018105 ISO
    establishment of cell polarity GO:0030010 ISO
    establishment of cell polarity GO:0030010 ISS
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 ISO
    intracellular signal transduction GO:0035556 ISS
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 ISO
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 ISS
    protein autophosphorylation GO:0046777 ISS
    regulation of axonogenesis GO:0050770 ISO
    regulation of axonogenesis GO:0050770 ISS
    regulation of cytoskeleton organization GO:0051493 ISO
    regulation of cytoskeleton organization GO:0051493 ISS
    axon development GO:0061564 ISO
    regulation of microtubule cytoskeleton organization GO:0070507 ISO
    establishment or maintenance of cell polarity regulating cell shape GO:0071963 ISO
    regulation of postsynapse organization GO:0099175 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    actin filament GO:0005884 ISO
    actin filament GO:0005884 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    lateral plasma membrane GO:0016328 ISO
    lateral plasma membrane GO:0016328 ISS
    dendrite GO:0030425 ISO
    dendrite GO:0030425 ISS
    basal cortex GO:0045180 IDA
    microtubule bundle GO:0097427 ISO
    microtubule bundle GO:0097427 ISS
    postsynapse GO:0098794 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Mark2 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mark2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNCA 6622
Affinity Capture-MS Homo sapiens
2 EEA1 8411
Affinity Capture-MS Homo sapiens
3 CLASP2 23122
Affinity Capture-MS Homo sapiens
4 Foxp3  
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 MTNR1A  
Reconstituted Complex Homo sapiens
7 CYTH1 9267
Affinity Capture-MS Homo sapiens
8 Ywhae 22627
Affinity Capture-MS Mus musculus
9 SRSF8 10929
Affinity Capture-MS Homo sapiens
10 PPHLN1  
Affinity Capture-MS Homo sapiens
11 Ywhab 54401
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
12 Ywhah 22629
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
13 SOGA1 140710
Affinity Capture-MS Homo sapiens
14 AKR7A2 8574
Affinity Capture-MS Homo sapiens
15 Hspa4 15525
Co-fractionation Mus musculus
16 Sstr3  
Proximity Label-MS Mus musculus
17 Eed  
Affinity Capture-MS Mus musculus
18 MTCL1 23255
Affinity Capture-MS Homo sapiens
19 Atg16l1  
Affinity Capture-MS Mus musculus
20 ERI3 79033
Affinity Capture-MS Homo sapiens
21 Ywhag 22628
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
22 RNF41  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Mark2 is involved
No pathways found





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