Gene description for Hsp90ab1
Gene name heat shock protein 90 alpha (cytosolic), class B member 1
Gene symbol Hsp90ab1
Other names/aliases 90kDa
AL022974
C81438
Hsp84
Hsp84-1
Hsp90
Hspcb
Species Mus musculus
 Database cross references - Hsp90ab1
ExoCarta ExoCarta_15516
Vesiclepedia VP_15516
Entrez Gene 15516
UniProt P11499  
 Hsp90ab1 identified in sEVs derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Colon cancer cells 37309723    
Dendritic cells 10545503    
Dendritic cells 11390481    
Embryonic fibroblasts 18494037    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 36648227    
Macrophages 36648227    
Macrophages 36648227    
Macrophages 36648227    
Macrophages 36648227    
Mast cells 17486113    
Neural stem cells 25242146    
Neural stem cells 25242146    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hsp90ab1
Molecular Function
    UTP binding GO:0002134 ISO
    CTP binding GO:0002135 ISO
    double-stranded RNA binding GO:0003725 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    GTP binding GO:0005525 ISO
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IEA
    sulfonylurea receptor binding GO:0017098 ISO
    protein kinase regulator activity GO:0019887 IMP
    kinase binding GO:0019900 IPI
    kinase binding GO:0019900 ISO
    protein kinase binding GO:0019901 IPI
    nitric-oxide synthase regulator activity GO:0030235 ISS
    TPR domain binding GO:0030911 ISS
    heat shock protein binding GO:0031072 IEA
    heat shock protein binding GO:0031072 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    dATP binding GO:0032564 ISO
    peptide binding GO:0042277 IEA
    peptide binding GO:0042277 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    histone deacetylase binding GO:0042826 IEA
    histone deacetylase binding GO:0042826 ISO
    ATP-dependent protein binding GO:0043008 IEA
    ATP-dependent protein binding GO:0043008 ISO
    protein folding chaperone GO:0044183 IMP
    transmembrane transporter binding GO:0044325 ISO
    protein dimerization activity GO:0046983 ISO
    protein dimerization activity GO:0046983 ISS
    tau protein binding GO:0048156 IPI
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IEA
    DNA polymerase binding GO:0070182 IEA
    DNA polymerase binding GO:0070182 ISO
    protein phosphatase activator activity GO:0072542 IEA
    disordered domain specific binding GO:0097718 IEA
    disordered domain specific binding GO:0097718 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
    receptor ligand inhibitor activity GO:0141069 ISO
    receptor ligand inhibitor activity GO:0141069 ISS
    heterocyclic compound binding GO:1901363 ISO
    histone methyltransferase binding GO:1990226 IEA
    histone methyltransferase binding GO:1990226 ISO
Biological Process
    placenta development GO:0001890 IMP
    protein folding GO:0006457 IBA
    protein folding GO:0006457 IMP
    telomere maintenance via telomerase GO:0007004 IEA
    virion attachment to host cell GO:0019062 IEA
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISS
    regulation of protein ubiquitination GO:0031396 IEA
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISS
    regulation of protein localization GO:0032880 IMP
    cellular response to heat GO:0034605 IBA
    cellular response to heat GO:0034605 IMP
    positive regulation of protein import into nucleus GO:0042307 ISO
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISS
    positive regulation of cell differentiation GO:0045597 IEA
    positive regulation of cell size GO:0045793 ISO
    protein stabilization GO:0050821 IBA
    chaperone-mediated protein complex assembly GO:0051131 IEA
    negative regulation of protein metabolic process GO:0051248 ISO
    regulation of cell cycle GO:0051726 ISS
    chaperone-mediated protein folding GO:0061077 IMP
    cellular response to interleukin-4 GO:0071353 IDA
    supramolecular fiber organization GO:0097435 IEA
    negative regulation of proteasomal protein catabolic process GO:1901799 IMP
    negative regulation of proteasomal protein catabolic process GO:1901799 ISO
    negative regulation of complement-dependent cytotoxicity GO:1903660 ISO
    telomerase holoenzyme complex assembly GO:1905323 IEA
    positive regulation of protein localization to cell surface GO:2000010 IEA
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular region GO:0005576 ISS
    intracellular anatomical structure GO:0005622 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    lysosomal membrane GO:0005765 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    COP9 signalosome GO:0008180 IEA
    COP9 signalosome GO:0008180 ISO
    cell surface GO:0009986 ISO
    inclusion body GO:0016234 ISO
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    brush border membrane GO:0031526 ISO
    protein-containing complex GO:0032991 IBA
    protein-containing complex GO:0032991 IDA
    protein-containing complex GO:0032991 ISO
    aryl hydrocarbon receptor complex GO:0034751 ISO
    aryl hydrocarbon receptor complex GO:0034751 ISS
    melanosome GO:0042470 IEA
    neuronal cell body GO:0043025 IDA
    dendritic growth cone GO:0044294 IDA
    axonal growth cone GO:0044295 IDA
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
    protein folding chaperone complex GO:0101031 IMP
    protein folding chaperone complex GO:0101031 ISO
    dynein axonemal particle GO:0120293 ISS
    HSP90-CDC37 chaperone complex GO:1990565 IDA
    HSP90-CDC37 chaperone complex GO:1990565 ISO
    sperm head plasma membrane GO:1990913 ISO
    ooplasm GO:1990917 ISO
 Experiment description of studies that identified Hsp90ab1 in sEVs
1
Experiment ID 89
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 3
MISEV standards
✔
IEM
Biophysical techniques
✔
HSP84|HSC73|MHCII|CD9|mac-1
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 10545503    
Organism Mus musculus
Experiment description Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
Authors "Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S."
Journal name JCB
Publication year 1999
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
4
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
5
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
6
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 569
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name RAW264.2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 570
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name RAW264.2 - E. coli infected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 571
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh|Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name RAW264.2 - E. coli infected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 580
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name BMDM
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
13
Experiment ID 581
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name BMDM
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
14
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
15
Experiment ID 262
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|HSP90|CD63|CD9|
Enriched markers
✘ Negative markers
✔
Y
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
16
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
18
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hsp90ab1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nod2  
Affinity Capture-Western Mus musculus
2 Sars2  
Co-fractionation Mus musculus
3 Hsf1  
Affinity Capture-Western Mus musculus
4 Hsp90ab1 15516
Affinity Capture-Western Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Affinity Capture-Western Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 Chordc1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
6 Akt1  
Affinity Capture-Western Mus musculus
7 Tmem173  
Proximity Label-MS Mus musculus
8 Fkbp5 14229
Co-fractionation Mus musculus
9 Nr3c2  
Affinity Capture-Western Mus musculus
10 Nr3c1  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
11 Gcgr  
Affinity Capture-Western Mus musculus
12 Stip1 20867
Co-fractionation Mus musculus
13 Sugt1 67955
Co-fractionation Mus musculus
14 Tsc1  
Affinity Capture-MS Mus musculus
15 DNAJB2 3300
Affinity Capture-Western Homo sapiens
16 Rpl6 19988
Co-fractionation Mus musculus
17 Kctd13  
Affinity Capture-MS Mus musculus
18 Tfe3  
Affinity Capture-MS Mus musculus
19 Htt  
Affinity Capture-Western Mus musculus
20 Irf3  
Affinity Capture-Western Mus musculus
21 Sgta 52551
Co-fractionation Mus musculus
22 Rps11 27207
Co-fractionation Mus musculus
23 Mks1  
Affinity Capture-MS Mus musculus
24 Iqcb1  
Affinity Capture-MS Mus musculus
25 Hdac6  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
26 Mt3  
Affinity Capture-MS Mus musculus
27 VASP 7408
Affinity Capture-MS Homo sapiens
28 Ywhaq 22630
Co-fractionation Mus musculus
29 Mettl21e  
Affinity Capture-MS Mus musculus
30 Nphp4  
Affinity Capture-MS Mus musculus
31 Fzr1  
Affinity Capture-MS Mus musculus
32 EIF4ENIF1 56478
Two-hybrid Homo sapiens
33 Nod1  
Affinity Capture-Western Mus musculus
34 Traf6  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
35 MAPT  
Reconstituted Complex Homo sapiens
36 CCNB1 891
Two-hybrid Homo sapiens
37 Cdkn2a  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
38 Unk  
Affinity Capture-RNA Mus musculus
39 Ywhab 54401
Co-fractionation Mus musculus
40 HACE1 57531
Affinity Capture-MS Homo sapiens
41 Invs  
Affinity Capture-MS Mus musculus
42 Hspa1b 15511
Reconstituted Complex Mus musculus
43 Rps13 68052
Co-fractionation Mus musculus
44 Slc34a1  
Two-hybrid Mus musculus
45 Ip6k2  
Affinity Capture-Western Mus musculus
46 Eed  
Affinity Capture-MS Mus musculus
47 Ywhae 22627
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
48 Ahi1  
Affinity Capture-MS Mus musculus
49 Nr1i3  
Affinity Capture-Western Mus musculus
50 Atg16l1  
Affinity Capture-MS Mus musculus
51 PRKACB  
Affinity Capture-MS Bos taurus
52 Nphp1  
Affinity Capture-MS Mus musculus
53 Rpl4 67891
Co-fractionation Mus musculus
54 Fancd2  
Affinity Capture-MS Mus musculus
55 Rpl8 26961
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Hsp90ab1 is involved
PathwayEvidenceSource
Aryl hydrocarbon receptor signalling IEA Reactome
Attenuation phase IEA Reactome
Axon guidance IEA Reactome
Biological oxidations IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
DDX58/IFIH1-mediated induction of interferon-alpha/beta IEA Reactome
Developmental Biology IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-dependent gene expression IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
G2/M Transition IEA Reactome
HSF1 activation IEA Reactome
HSF1-dependent transactivation IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Inflammasomes IEA Reactome
Innate Immune System IEA Reactome
Metabolism IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Nervous system development IEA Reactome
Neutrophil degranulation IEA Reactome
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways IEA Reactome
Phase I - Functionalization of compounds IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle IEA Reactome
RHOBTB GTPase Cycle IEA Reactome
RHOBTB2 GTPase cycle IEA Reactome
Sema3A PAK dependent Axon repulsion IEA Reactome
Semaphorin interactions IEA Reactome
Signal Transduction IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
The NLRP3 inflammasome IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here