Gene description for Stip1
Gene name stress-induced phosphoprotein 1
Gene symbol Stip1
Other names/aliases Hop
Sti1
p60
Species Mus musculus
 Database cross references - Stip1
ExoCarta ExoCarta_20867
Vesiclepedia VP_20867
Entrez Gene 20867
UniProt Q60864  
 Stip1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stip1
Molecular Function
    protein binding GO:0005515 IPI
    Hsp70 protein binding GO:0030544 ISO
    protein-folding chaperone binding GO:0051087 ISO
    Hsp90 protein binding GO:0051879 IBA
    Hsp90 protein binding GO:0051879 IEA
    Hsp90 protein binding GO:0051879 ISO
Biological Process
    cellular response to interleukin-7 GO:0098761 IDA
Subcellular Localization
    nucleus GO:0005634 IPI
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 ISO
    myelin sheath GO:0043209 HDA
    protein folding chaperone complex GO:0101031 IEA
    protein folding chaperone complex GO:0101031 ISO
    dynein axonemal particle GO:0120293 ISS
 Experiment description of studies that identified Stip1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stip1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hsp90aa1 15519
Co-fractionation Mus musculus
2 Ahsa1 217737
Co-fractionation Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Syngap1  
Affinity Capture-MS Mus musculus
5 Hsp90ab1 15516
Co-fractionation Mus musculus
6 Fmr1  
Affinity Capture-MS Mus musculus
7 Hspa4l  
Co-fractionation Mus musculus
8 Hspa8 15481
Co-fractionation Mus musculus
9 Tfe3  
Affinity Capture-MS Mus musculus
10 Atxn1  
Proximity Label-MS Mus musculus
11 Mllt3  
Affinity Capture-Western Mus musculus
12 Cops4 26891
Co-fractionation Mus musculus
13 Aip  
Co-fractionation Mus musculus
14 Gnb2 14693
Co-fractionation Mus musculus
15 Fancd2  
Affinity Capture-MS Mus musculus
16 Fkbp4 14228
Co-fractionation Mus musculus
17 Tcf3  
Affinity Capture-MS Mus musculus
18 Kcnma1  
Affinity Capture-MS Mus musculus
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