Gene description for Hspa1b
Gene name heat shock protein 1B
Gene symbol Hspa1b
Other names/aliases Hsp70
Hsp70-1
Hsp70.1
hsp68
Species Mus musculus
 Database cross references - Hspa1b
ExoCarta ExoCarta_15511
Vesiclepedia VP_15511
Entrez Gene 15511
UniProt P17879  
 Hspa1b identified in sEVs derived from the following tissue/cell type
Breast cancer cells 34112803    
Breast cancer cells 34112803    
Embryonic fibroblasts 18494037    
Hepatocytes 34489604    
Hepatocytes 34489604    
Hepatocytes 34489604    
Neural stem cells 25242146    
Oligodendrocytes 21136642    
Serum 35173168    
 Gene ontology annotations for Hspa1b
Molecular Function
    G protein-coupled receptor binding GO:0001664 ISO
    protease binding GO:0002020 ISO
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 ISO
    enzyme binding GO:0019899 ISO
    heat shock protein binding GO:0031072 IBA
    heat shock protein binding GO:0031072 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    histone deacetylase binding GO:0042826 ISO
    protein folding chaperone GO:0044183 IBA
    protein folding chaperone GO:0044183 IDA
    protein folding chaperone GO:0044183 ISO
    NF-kappaB binding GO:0051059 ISO
    unfolded protein binding GO:0051082 ISO
    C3HC4-type RING finger domain binding GO:0055131 ISO
    ATP-dependent protein disaggregase activity GO:0140545 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    mRNA catabolic process GO:0006402 ISO
    protein folding GO:0006457 IDA
    binding of sperm to zona pellucida GO:0007339 IDA
    negative regulation of cell population proliferation GO:0008285 ISO
    response to heat GO:0009408 IDA
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of cell growth GO:0030308 ISO
    regulation of protein ubiquitination GO:0031396 ISO
    negative regulation of protein ubiquitination GO:0031397 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IBA
    positive regulation of interleukin-8 production GO:0032757 ISO
    cellular response to heat GO:0034605 ISO
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 ISO
    protein refolding GO:0042026 ISS
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of erythrocyte differentiation GO:0045648 ISO
    ATP metabolic process GO:0046034 ISO
    protein stabilization GO:0050821 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    cellular heat acclimation GO:0070370 ISO
    positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway GO:0070434 ISO
    positive regulation of microtubule nucleation GO:0090063 ISO
    positive regulation of microtubule nucleation GO:0090063 ISS
    negative regulation of inclusion body assembly GO:0090084 ISO
    regulation of mitotic spindle assembly GO:1901673 ISO
    regulation of mitotic spindle assembly GO:1901673 ISS
    positive regulation of tumor necrosis factor-mediated signaling pathway GO:1903265 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 IDA
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 ISO
Subcellular Localization
    zona pellucida receptor complex GO:0002199 IDA
    intracellular anatomical structure GO:0005622 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISS
    mitochondrial matrix GO:0005759 ISO
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    centriole GO:0005814 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    inclusion body GO:0016234 ISO
    aggresome GO:0016235 ISO
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    nuclear speck GO:0016607 ISO
    protein-containing complex GO:0032991 ISO
    cell body GO:0044297 IDA
    membrane raft GO:0045121 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Hspa1b in sEVs
1
Experiment ID 520
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Mus musculus
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name EO771
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 521
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Mus musculus
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name PyMT
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 73
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
4
Experiment ID 695
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
5
Experiment ID 696
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
6
Experiment ID 697
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
7
Experiment ID 262
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
8
Experiment ID 33
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
9
Experiment ID 870
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35173168    
Organism Mus musculus
Experiment description Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression
Authors "Qi M, Xia Y, Wu Y, Zhang Z, Wang X, Lu L, Dai C, Song Y, Xu K, Ji W, Zhan L."
Journal name Nat Commun
Publication year 2022
Sample Serum
Sample name BALB/c bearing 4T07 primary tumour
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study Western blotting
RNA sequencing
qRT-PCR
 Protein-protein interactions for Hspa1b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Krt8 16691
Affinity Capture-Western Mus musculus
2 Ighmbp2 20589
Affinity Capture-MS Mus musculus
3 Nr3c1  
Affinity Capture-Western Mus musculus
4 SOD1 6647
Affinity Capture-MS Homo sapiens
5 Hif1a  
Affinity Capture-Western Mus musculus
6 Il4ra  
Affinity Capture-Western Mus musculus
7 Trp53  
Affinity Capture-Western Mus musculus
8 Erg  
Affinity Capture-Western Mus musculus
9 DNAJB2 3300
Affinity Capture-Western Homo sapiens
10 Stub1 56424
Affinity Capture-Western Mus musculus
11 Foxp3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 Hsp90ab1 15516
Reconstituted Complex Mus musculus
13 Pfn1 18643
Affinity Capture-MS Mus musculus
14 Htt  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
15 Mllt3  
Affinity Capture-Western Mus musculus
16 Bag4  
Affinity Capture-Western Mus musculus
17 Bag3  
Affinity Capture-Western Mus musculus
18 Dnajb6 23950
Affinity Capture-Western Mus musculus
19 Mt3  
Affinity Capture-MS Mus musculus
20 Ppp1ca 19045
Affinity Capture-Western Mus musculus
21 Atxn3  
Affinity Capture-Western Mus musculus
22 GZMB  
Biochemical Activity Homo sapiens
23 Grin2b  
Affinity Capture-Western Mus musculus
24 DYX1C1  
Affinity Capture-Western Homo sapiens
25 Traf6  
Affinity Capture-Western Mus musculus
26 Epm2a  
Affinity Capture-Western Mus musculus
27 Ppp1cc 19047
Affinity Capture-Western Mus musculus
28 Grin1  
Affinity Capture-Western Mus musculus
29 Ikbkg  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
30 Lnx1  
Affinity Capture-MS Mus musculus
31 Kcnb1  
Affinity Capture-Western Mus musculus
32 Bag5 70369
Affinity Capture-Western Mus musculus
33 ACTB 60
Reconstituted Complex Homo sapiens
34 Nhlrc1  
Affinity Capture-Western Mus musculus
35 Ppp1cb 19046
Affinity Capture-Western Mus musculus
36 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
37 Pfn2  
Affinity Capture-MS Mus musculus
38 Socs1  
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here