Gene description for Bag5
Gene name BCL2-associated athanogene 5
Gene symbol Bag5
Other names/aliases 1600025G07Rik
4930405J06Rik
Species Mus musculus
 Database cross references - Bag5
ExoCarta ExoCarta_70369
Vesiclepedia VP_70369
Entrez Gene 70369
UniProt Q8CI32  
 Bag5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Bag5
Molecular Function
    adenyl-nucleotide exchange factor activity GO:0000774 IBA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 ISO
    protein-folding chaperone binding GO:0051087 ISS
Biological Process
    Golgi organization GO:0007030 ISO
    Golgi organization GO:0007030 ISS
    negative regulation of neuron projection development GO:0010977 ISO
    negative regulation of neuron projection development GO:0010977 ISS
    negative regulation of protein ubiquitination GO:0031397 IBA
    negative regulation of protein ubiquitination GO:0031397 ISO
    negative regulation of protein ubiquitination GO:0031397 ISS
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISS
    protein stabilization GO:0050821 IBA
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    negative regulation of ubiquitin-protein transferase activity GO:0051444 ISS
    negative regulation of protein refolding GO:0061084 ISO
    negative regulation of protein refolding GO:0061084 ISS
    regulation of inclusion body assembly GO:0090083 IBA
    regulation of inclusion body assembly GO:0090083 ISO
    regulation of inclusion body assembly GO:0090083 ISS
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISO
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    membrane GO:0016020 IBA
    inclusion body GO:0016234 ISO
    inclusion body GO:0016234 ISS
    junctional membrane complex GO:0030314 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
 Experiment description of studies that identified Bag5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Bag5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hspa1b 15511
Affinity Capture-Western Mus musculus
2 Frmd5  
Co-fractionation Mus musculus
3 Gak  
Reconstituted Complex Mus musculus
4 Stub1 56424
Affinity Capture-Western Mus musculus
5 Wdr45  
Co-fractionation Mus musculus
6 Lrrk2  
Affinity Capture-Western Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here