Gene description for Dnajb6
Gene name DnaJ (Hsp40) homolog, subfamily B, member 6
Gene symbol Dnajb6
Other names/aliases Mrj
mDj4
Species Mus musculus
 Database cross references - Dnajb6
ExoCarta ExoCarta_23950
Vesiclepedia VP_23950
Entrez Gene 23950
UniProt O54946  
 Dnajb6 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Dnajb6
Molecular Function
    ATPase activator activity GO:0001671 IEA
    ATPase activator activity GO:0001671 ISO
    DNA binding GO:0003677 IDA
    protein binding GO:0005515 IPI
    Hsp70 protein binding GO:0030544 IEA
    heat shock protein binding GO:0031072 ISO
    identical protein binding GO:0042802 ISO
    protein folding chaperone GO:0044183 IBA
    protein folding chaperone GO:0044183 ISO
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IEA
    unfolded protein binding GO:0051082 ISO
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 ISO
Biological Process
    protein folding GO:0006457 ISO
    actin cytoskeleton organization GO:0030036 IMP
    extracellular matrix organization GO:0030198 IMP
    regulation of protein localization GO:0032880 IEA
    regulation of protein localization GO:0032880 ISO
    protein localization to nucleus GO:0034504 IMP
    intermediate filament organization GO:0045109 IEA
    intermediate filament organization GO:0045109 ISO
    negative regulation of DNA-templated transcription GO:0045892 IDA
    chorio-allantoic fusion GO:0060710 IMP
    syncytiotrophoblast cell differentiation involved in labyrinthine layer development GO:0060715 IMP
    chorion development GO:0060717 IMP
    chaperone-mediated protein folding GO:0061077 IBA
    chaperone-mediated protein folding GO:0061077 ISO
    negative regulation of inclusion body assembly GO:0090084 IEA
    negative regulation of inclusion body assembly GO:0090084 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    Z disc GO:0030018 IEA
    Z disc GO:0030018 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Dnajb6 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Dnajb6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hspa1b 15511
Affinity Capture-Western Mus musculus
2 Zbtb7b  
Affinity Capture-MS Mus musculus
3 Nphs2  
Affinity Capture-MS Mus musculus
4 Ubc  
Reconstituted Complex Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Nfatc3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
7 Foxp3  
Affinity Capture-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Hdac7  
Affinity Capture-Western Mus musculus
10 Atg16l1  
Affinity Capture-MS Mus musculus
11 Hdac4  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 Twist1  
Proximity Label-MS Mus musculus
13 Fancd2  
Affinity Capture-MS Mus musculus
14 Hdac5  
Affinity Capture-Western Mus musculus
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