Gene description for Nedd4
Gene name neural precursor cell expressed, developmentally down-regulated 4
Gene symbol Nedd4
Other names/aliases AA959633
AL023035
AU019897
E430025J12Rik
Nedd4-1
Nedd4a
mKIAA0093
Species Mus musculus
 Database cross references - Nedd4
ExoCarta ExoCarta_17999
Vesiclepedia VP_17999
Entrez Gene 17999
UniProt P46935  
 Nedd4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nedd4
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
    protein binding GO:0005515 IPI
    sodium channel inhibitor activity GO:0019871 IBA
    sodium channel inhibitor activity GO:0019871 IDA
    sodium channel inhibitor activity GO:0019871 ISO
    enzyme binding GO:0019899 ISO
    protein domain specific binding GO:0019904 ISO
    beta-2 adrenergic receptor binding GO:0031698 ISO
    ionotropic glutamate receptor binding GO:0035255 IDA
    ubiquitin binding GO:0043130 ISO
    phosphoserine residue binding GO:0050815 IDA
    phosphothreonine residue binding GO:0050816 IDA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin protein ligase activity GO:0061630 ISO
    RNA polymerase binding GO:0070063 ISO
    proline-rich region binding GO:0070064 IPI
    proline-rich region binding GO:0070064 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    adaptive immune response GO:0002250 IMP
    outflow tract morphogenesis GO:0003151 IGI
    outflow tract morphogenesis GO:0003151 IMP
    endocardial cushion development GO:0003197 IGI
    endocardial cushion development GO:0003197 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    protein monoubiquitination GO:0006513 IDA
    protein targeting to lysosome GO:0006622 ISO
    sodium ion transport GO:0006814 IDA
    DNA damage response GO:0006974 ISO
    lysosomal transport GO:0007041 ISO
    nervous system development GO:0007399 IEA
    neuromuscular junction development GO:0007528 IBA
    neuromuscular junction development GO:0007528 IMP
    negative regulation of sodium ion transport GO:0010766 IDA
    negative regulation of sodium ion transport GO:0010766 ISO
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 ISO
    negative regulation of vascular endothelial growth factor receptor signaling pathway GO:0030948 IDA
    neuron projection development GO:0031175 IBA
    receptor internalization GO:0031623 IBA
    receptor internalization GO:0031623 ISO
    receptor catabolic process GO:0032801 IBA
    receptor catabolic process GO:0032801 ISO
    cellular response to UV GO:0034644 ISO
    regulation of monoatomic ion transmembrane transport GO:0034765 ISO
    T cell activation GO:0042110 IMP
    regulation of membrane potential GO:0042391 ISO
    nuclear receptor-mediated glucocorticoid signaling pathway GO:0042921 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    formation of structure involved in a symbiotic process GO:0044111 ISO
    innate immune response GO:0045087 ISO
    positive regulation of protein catabolic process GO:0045732 IDA
    positive regulation of protein catabolic process GO:0045732 ISO
    viral budding GO:0046755 ISO
    positive regulation of nucleocytoplasmic transport GO:0046824 ISO
    blood vessel morphogenesis GO:0048514 IGI
    blood vessel morphogenesis GO:0048514 IMP
    regulation of dendrite morphogenesis GO:0048814 IBA
    regulation of dendrite morphogenesis GO:0048814 IMP
    regulation of dendrite morphogenesis GO:0048814 ISO
    regulation of synapse organization GO:0050807 IBA
    regulation of synapse organization GO:0050807 IMP
    progesterone receptor signaling pathway GO:0050847 ISO
    establishment of localization in cell GO:0051649 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K63-linked ubiquitination GO:0070534 ISS
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 ISO
    regulation of protein catabolic process at postsynapse, modulating synaptic transmission GO:0099576 ISO
    regulation protein catabolic process at postsynapse GO:0140252 ISO
    regulation of potassium ion transmembrane transporter activity GO:1901016 ISO
    negative regulation of sodium ion transmembrane transporter activity GO:2000650 ISO
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IPI
    chromatin GO:0000785 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    microvillus GO:0005902 ISO
    cell cortex GO:0005938 ISO
    membrane GO:0016020 IDA
    protein-containing complex GO:0032991 ISO
    dendritic spine GO:0043197 IBA
    perinuclear region of cytoplasm GO:0048471 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
    postsynaptic cytosol GO:0099524 IDA
    postsynaptic cytosol GO:0099524 IMP
 Experiment description of studies that identified Nedd4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nedd4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bean1  
Far Western Mus musculus
Far Western Mus musculus
2 Pmepa1  
Far Western Mus musculus
Far Western Mus musculus
3 Fxr1 14359
Affinity Capture-MS Mus musculus
4 Tnik  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
5 Usp2  
Reconstituted Complex Mus musculus
6 Spdya  
Affinity Capture-Western Mus musculus
7 Rnf11  
Far Western Mus musculus
Far Western Mus musculus
8 GUCD1  
Two-hybrid Homo sapiens
9 Aicda  
Affinity Capture-Western Mus musculus
10 Traf2  
Affinity Capture-Western Mus musculus
11 Pou5f1  
Affinity Capture-MS Mus musculus
12 Litaf  
Far Western Mus musculus
Far Western Mus musculus
13 Ubox5  
Co-fractionation Mus musculus
14 Ndfip1  
Far Western Mus musculus
Affinity Capture-Western Mus musculus
Far Western Mus musculus
Far Western Mus musculus
15 Dlg3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
16 Sftpc  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
17 N4bp1  
Biochemical Activity Mus musculus
18 Prrg2  
Far Western Mus musculus
Far Western Mus musculus
19 Scnn1b  
Affinity Capture-Western Mus musculus
Far Western Mus musculus
Co-fractionation Mus musculus
20 Fmr1  
Affinity Capture-MS Mus musculus
21 Scnn1g  
Far Western Mus musculus
22 GLIS3  
Two-hybrid Homo sapiens
23 Sulf1  
Co-fractionation Mus musculus
24 Vdac1 22333
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
25 Gabarapl2  
Co-fractionation Mus musculus
26 Irgq  
Co-fractionation Mus musculus
27 Shank3  
Affinity Capture-MS Mus musculus
28 Scnn1a  
Affinity Capture-Western Mus musculus
Far Western Mus musculus
Co-fractionation Mus musculus
29 Wbp2  
Far Western Mus musculus
Far Western Mus musculus
30 Hmces  
Co-fractionation Mus musculus
31 N4BP3  
Reconstituted Complex Homo sapiens
32 Cd40 21939
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
33 Ywhae 22627
Affinity Capture-Western Mus musculus
34 POLR2A 5430
Affinity Capture-Western Homo sapiens
35 NFE2  
Far Western Homo sapiens
36 SCN3A  
Reconstituted Complex Homo sapiens
37 Adrbk1 110355
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
38 UBE2D2 7322
Reconstituted Complex Homo sapiens
39 Wbp1  
Far Western Mus musculus
Far Western Mus musculus
40 Laptm5 16792
Reconstituted Complex Mus musculus
41 Ywhab 54401
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
42 Agap2  
Affinity Capture-MS Mus musculus
43 LDLRAD3  
Reconstituted Complex Homo sapiens
44 UBE2L3 7332
Reconstituted Complex Homo sapiens
45 UBE2D3 7323
Reconstituted Complex Homo sapiens
46 Kcp  
Co-fractionation Mus musculus
47 Tsc1  
Affinity Capture-MS Mus musculus
48 Nfe2l2  
Affinity Capture-Western Mus musculus
49 Sqstm1  
Affinity Capture-MS Mus musculus
50 Nedd4 17999
Biochemical Activity Mus musculus
Biochemical Activity Mus musculus
51 Eed  
Affinity Capture-MS Mus musculus
52 Fnbp1  
Co-fractionation Mus musculus
53 Traf3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
54 Prr16  
Co-fractionation Mus musculus
55 Grb10  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Co-crystal Structure Mus musculus
56 Rap2a  
Affinity Capture-Western Mus musculus
57 Gpbar1  
Affinity Capture-MS Mus musculus
58 SCN5A 6331
Reconstituted Complex Homo sapiens
59 Cbl 12402
Affinity Capture-Western Mus musculus
60 Fancd2  
Affinity Capture-MS Mus musculus
61 Scn2a  
Reconstituted Complex Rattus norvegicus
62 UBE2D1 7321
Reconstituted Complex Homo sapiens
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