Gene description for Cbl
Gene name Casitas B-lineage lymphoma
Gene symbol Cbl
Other names/aliases 4732447J05Rik
Cbl-2
c-Cbl
cbl
Species Mus musculus
 Database cross references - Cbl
ExoCarta ExoCarta_12402
Vesiclepedia VP_12402
Entrez Gene 12402
UniProt P22682  
 Cbl identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cbl
Molecular Function
    phosphotyrosine residue binding GO:0001784 IEA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IPI
    SH3 domain binding GO:0017124 ISO
    protein kinase binding GO:0019901 ISO
    receptor tyrosine kinase binding GO:0030971 IBA
    phosphatidylinositol 3-kinase regulatory subunit binding GO:0036312 IEA
    phosphatidylinositol 3-kinase regulatory subunit binding GO:0036312 ISO
    ephrin receptor binding GO:0046875 IPI
    ephrin receptor binding GO:0046875 ISO
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin protein ligase activity GO:0061630 IGI
    ubiquitin protein ligase activity GO:0061630 IMP
    ubiquitin protein ligase activity GO:0061630 ISO
    ubiquitin protein ligase activity GO:0061630 TAS
    protein tyrosine kinase binding GO:1990782 IEA
    protein tyrosine kinase binding GO:1990782 ISO
Biological Process
    protein polyubiquitination GO:0000209 IEA
    protein polyubiquitination GO:0000209 ISO
    protein phosphorylation GO:0006468 ISS
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    protein monoubiquitination GO:0006513 IEA
    protein monoubiquitination GO:0006513 ISO
    DNA damage response GO:0006974 IEA
    signal transduction GO:0007165 IBA
    cell surface receptor signaling pathway GO:0007166 IEA
    male gonad development GO:0008584 IEA
    response to gamma radiation GO:0010332 IEA
    response to activity GO:0014823 IEA
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 ISS
    regulation of Rap protein signal transduction GO:0032487 IMP
    response to testosterone GO:0033574 IEA
    cellular response to platelet-derived growth factor stimulus GO:0036120 IEA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IBA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IDA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IGI
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IMP
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 ISO
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 ISS
    response to starvation GO:0042594 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    mast cell degranulation GO:0043303 IEA
    bone resorption GO:0045453 ISS
    response to ethanol GO:0045471 IEA
    positive regulation of receptor-mediated endocytosis GO:0048260 ISO
    positive regulation of receptor-mediated endocytosis GO:0048260 ISS
    protein autoubiquitination GO:0051865 IEA
    protein autoubiquitination GO:0051865 ISO
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    ubiquitin-dependent endocytosis GO:0070086 IMP
    ubiquitin-dependent endocytosis GO:0070086 ISO
    cellular response to hypoxia GO:0071456 IEA
    cellular response to nerve growth factor stimulus GO:1990090 IEA
    regulation of platelet-derived growth factor receptor-alpha signaling pathway GO:2000583 IMP
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    cilium GO:0005929 IDA
    cilium GO:0005929 ISO
    flotillin complex GO:0016600 IDA
    axon GO:0030424 ISO
    growth cone GO:0030426 IEA
    growth cone GO:0030426 ISO
    membrane raft GO:0045121 IBA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Cbl in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cbl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eps8  
Affinity Capture-Western Mus musculus
2 Syk  
Affinity Capture-Western Mus musculus
3 Pdgfrb 18596
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
4 Sla2 77799
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Atxn2 20239
Affinity Capture-Western Mus musculus
6 Grb2 14784
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
7 Sh3gl2  
Co-fractionation Mus musculus
8 Cd2ap 12488
Affinity Capture-Western Mus musculus
9 Sla 20491
Affinity Capture-Western Mus musculus
10 Serpinb9  
Affinity Capture-Western Mus musculus
11 Lyn 17096
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 Ptk2b  
Co-fractionation Mus musculus
13 Kit  
Affinity Capture-Western Mus musculus
14 Sorbs2  
Reconstituted Complex Mus musculus
15 Itk  
Affinity Capture-Western Mus musculus
16 Map1lc3b  
Affinity Capture-Western Mus musculus
17 Pik3r1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
18 Tnfrsf11a  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
19 Fyn  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
20 Crkl 12929
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
21 EGFR 1956
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 Plcg1  
Affinity Capture-Western Mus musculus
23 Shc1  
Affinity Capture-Western Mus musculus
24 Dok3  
Affinity Capture-Western Mus musculus
25 Dlg1 13383
Affinity Capture-Western Mus musculus
26 Nfatc1  
Affinity Capture-Western Mus musculus
27 Cd40 21939
Affinity Capture-Western Mus musculus
28 Ywhae 22627
Affinity Capture-MS Mus musculus
29 Fgr  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
30 Itsn1 16443
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
31 Inpp5d 16331
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
32 Tmem173  
Proximity Label-MS Mus musculus
33 Flt1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
34 Hck  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
Far Western Mus musculus
35 Synm  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
36 Spta1  
Affinity Capture-Western Mus musculus
37 Erbb2  
Affinity Capture-Western Mus musculus
38 Src 20779
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
39 Traf6  
Affinity Capture-Western Mus musculus
40 Atm  
Affinity Capture-Western Mus musculus
41 Sh3kbp1 58194
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
42 Abl1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
43 Nck1 17973
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
44 Lck 16818
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
45 Crk 12928
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
46 Pik3cg  
Co-purification Mus musculus
47 Sh2b2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Protein-peptide Mus musculus
48 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
49 Nedd4 17999
Affinity Capture-Western Mus musculus
50 SYK 6850
Affinity Capture-Western Homo sapiens
51 Egfr 13649
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Protein-peptide Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
52 Zap70 22637
Affinity Capture-Western Mus musculus
Protein-peptide Mus musculus
Affinity Capture-Western Mus musculus
Protein-peptide Mus musculus
53 Rapgef1  
Affinity Capture-Western Mus musculus
54 Sorbs1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
55 Sos1  
Co-fractionation Mus musculus
56 Tgfbr2 21813
Affinity Capture-Western Mus musculus
57 Pxn  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
58 Ptpn11 19247
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Cbl is involved
PathwayEvidenceSource
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
EGFR downregulation IEA Reactome
FLT3 Signaling IEA Reactome
Immune System IEA Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling IEA Reactome
Interleukin-6 family signaling IEA Reactome
Interleukin-6 signaling IEA Reactome
Membrane Trafficking IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulation of FLT3 IEA Reactome
Negative regulation of MET activity IEA Reactome
PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 IEA Reactome
Regulation of KIT signaling IEA Reactome
Regulation of signaling by CBL IEA Reactome
Signal Transduction IEA Reactome
Signaling by EGFR IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by MET IEA Reactome
Signaling by Non-Receptor Tyrosine Kinases IEA Reactome
Signaling by PTK6 IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by SCF-KIT IEA Reactome
Signaling by TGF-beta Receptor Complex IEA Reactome
Signaling by TGFB family members IEA Reactome
Spry regulation of FGF signaling IEA Reactome
TGF-beta receptor signaling activates SMADs IEA Reactome
Vesicle-mediated transport IEA Reactome





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