Gene description for Crkl
Gene name v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Gene symbol Crkl
Other names/aliases 1110025F07Rik
AA589403
AI325100
Crkol
Species Mus musculus
 Database cross references - Crkl
ExoCarta ExoCarta_12929
Vesiclepedia VP_12929
Entrez Gene 12929
UniProt P47941  
 Crkl identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Crkl
Molecular Function
    phosphotyrosine residue binding GO:0001784 IEA
    phosphotyrosine residue binding GO:0001784 ISO
    protein binding GO:0005515 IPI
    receptor tyrosine kinase binding GO:0030971 IBA
    signaling adaptor activity GO:0035591 IBA
    signaling adaptor activity GO:0035591 IGI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
Biological Process
    regulation of cell growth GO:0001558 IDA
    blood vessel development GO:0001568 IGI
    urogenital system development GO:0001655 IMP
    neuron migration GO:0001764 IGI
    neuron migration GO:0001764 IMP
    B cell apoptotic process GO:0001783 IMP
    negative regulation of protein phosphorylation GO:0001933 ISO
    positive regulation of protein phosphorylation GO:0001934 ISO
    regulation of leukocyte migration GO:0002685 IGI
    outflow tract morphogenesis GO:0003151 IMP
    lipid metabolic process GO:0006629 IGI
    enzyme-linked receptor protein signaling pathway GO:0007167 IBA
    enzyme-linked receptor protein signaling pathway GO:0007167 IDA
    enzyme-linked receptor protein signaling pathway GO:0007167 IGI
    enzyme-linked receptor protein signaling pathway GO:0007167 IMP
    spermatogenesis GO:0007283 IMP
    single fertilization GO:0007338 IMP
    pattern specification process GO:0007389 IGI
    heart development GO:0007507 IGI
    positive regulation of cell population proliferation GO:0008284 IEA
    positive regulation of cell population proliferation GO:0008284 ISO
    fibroblast growth factor receptor signaling pathway GO:0008543 IMP
    male gonad development GO:0008584 IMP
    animal organ morphogenesis GO:0009887 IMP
    anterior/posterior pattern specification GO:0009952 IGI
    regulation of gene expression GO:0010468 IMP
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    dendrite development GO:0016358 IGI
    cell migration GO:0016477 IBA
    hippocampus development GO:0021766 IGI
    cerebral cortex development GO:0021987 IGI
    establishment of cell polarity GO:0030010 IGI
    regulation of cell adhesion mediated by integrin GO:0033628 IGI
    positive regulation of Rac protein signal transduction GO:0035022 IMP
    helper T cell diapedesis GO:0035685 IGI
    reelin-mediated signaling pathway GO:0038026 IGI
    reelin-mediated signaling pathway GO:0038026 IMP
    positive regulation of MAPK cascade GO:0043410 IDA
    retinoic acid receptor signaling pathway GO:0048384 IMP
    thymus development GO:0048538 IGI
    regulation of dendrite development GO:0050773 IGI
    T cell receptor signaling pathway GO:0050852 IDA
    parathyroid gland development GO:0060017 IGI
    cell chemotaxis GO:0060326 IGI
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IBA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    cellular response to xenobiotic stimulus GO:0071466 IDA
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 ISO
    response to fibroblast growth factor GO:0071774 IMP
    endothelin receptor signaling pathway GO:0086100 IMP
    activation of GTPase activity GO:0090630 IGI
    acetylcholine receptor signaling pathway GO:0095500 IGI
    postsynaptic specialization assembly GO:0098698 IDA
    postsynaptic specialization assembly GO:0098698 IMP
    cerebellar neuron development GO:0098749 IGI
    cellular response to interleukin-7 GO:0098761 IDA
    chordate pharynx development GO:0160093 IMP
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    positive regulation of glial cell migration GO:1903977 IEA
    positive regulation of glial cell migration GO:1903977 ISO
    regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904393 IGI
    positive regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904395 IDA
    positive regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904395 IGI
    positive regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904395 IMP
    cranial skeletal system development GO:1904888 IMP
    regulation of T cell migration GO:2000404 IGI
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    neuromuscular junction GO:0031594 IDA
    neuromuscular junction GO:0031594 IMP
    protein-containing complex GO:0032991 IDA
    synapse GO:0045202 IDA
    extrinsic component of postsynaptic membrane GO:0098890 IDA
 Experiment description of studies that identified Crkl in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Crkl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cblb 208650
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 Sos1  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
3 Pxn  
Affinity Capture-Western Mus musculus
4 Inpp5d 16331
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
5 Atxn1  
Proximity Label-MS Mus musculus
6 Hspd1 15510
Affinity Capture-MS Mus musculus
7 Rapgef1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
8 Bcar1  
Affinity Capture-Western Mus musculus
9 Zap70 22637
Affinity Capture-Western Mus musculus
10 Dab1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
11 Fgfr1 14182
Affinity Capture-Western Mus musculus
12 Prdx1 18477
Affinity Capture-MS Mus musculus
13 Nckipsd  
Affinity Capture-MS Mus musculus
14 Abl1  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
15 Fancd2  
Affinity Capture-MS Mus musculus
16 Pik3r1  
Reconstituted Complex Mus musculus
17 Cbl 12402
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
18 Fgfr2  
Affinity Capture-Western Mus musculus
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