Gene description for Prdx1
Gene name peroxiredoxin 1
Gene symbol Prdx1
Other names/aliases MSP23
NkefA
OSF-3
OSF3
PAG
Paga
PrdxI
PrxI
TDX2
TPxA
Tdpx2
prx1
Species Mus musculus
 Database cross references - Prdx1
ExoCarta ExoCarta_18477
Vesiclepedia VP_18477
Entrez Gene 18477
UniProt P35700  
 Prdx1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 11390481    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prdx1
Molecular Function
    peroxidase activity GO:0004601 ISO
    protein binding GO:0005515 IPI
    thioredoxin peroxidase activity GO:0008379 IBA
    thioredoxin peroxidase activity GO:0008379 IEA
    thioredoxin peroxidase activity GO:0008379 ISO
    heme binding GO:0020037 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    peroxiredoxin activity GO:0051920 ISO
Biological Process
    response to reactive oxygen species GO:0000302 IMP
    response to oxidative stress GO:0006979 IBA
    response to oxidative stress GO:0006979 IDA
    response to oxidative stress GO:0006979 IMP
    canonical NF-kappaB signal transduction GO:0007249 IMP
    removal of superoxide radicals GO:0019430 IBA
    removal of superoxide radicals GO:0019430 IMP
    natural killer cell activation GO:0030101 IEA
    natural killer cell activation GO:0030101 ISO
    regulation of stress-activated MAPK cascade GO:0032872 IMP
    erythrocyte homeostasis GO:0034101 IMP
    natural killer cell mediated cytotoxicity GO:0042267 IMP
    hydrogen peroxide catabolic process GO:0042744 IBA
    hydrogen peroxide catabolic process GO:0042744 ISO
    hydrogen peroxide catabolic process GO:0042744 ISO
    leukocyte activation GO:0045321 IBA
    cell redox homeostasis GO:0045454 IBA
    fibroblast proliferation GO:0048144 IMP
    regulation of non-canonical NF-kappaB signal transduction GO:1901222 IMP
Subcellular Localization
    euchromatin GO:0000791 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    mitochondrial matrix GO:0005759 ISO
    peroxisomal matrix GO:0005782 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Prdx1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
8
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prdx1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZMYM1 79830
Affinity Capture-MS Homo sapiens
2 LSM5  
Two-hybrid Homo sapiens
3 FDX1 2230
Affinity Capture-MS Homo sapiens
4 Tmem173  
Proximity Label-MS Mus musculus
5 Nphp4  
Affinity Capture-MS Mus musculus
6 Srxn1  
Co-fractionation Mus musculus
7 TXNDC5 81567
Affinity Capture-MS Homo sapiens
8 Eed  
Affinity Capture-MS Mus musculus
9 MAPT  
Reconstituted Complex Homo sapiens
10 CCNB1 891
Two-hybrid Homo sapiens
11 Crkl 12929
Affinity Capture-MS Mus musculus
12 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
13 Tfe3  
Affinity Capture-MS Mus musculus
14 PRDX2 7001
Affinity Capture-MS Homo sapiens
15 TRIO 7204
Affinity Capture-MS Homo sapiens
16 Ube3a  
Co-localization Mus musculus
17 HSD17B10 3028
Affinity Capture-MS Homo sapiens
18 RAD51  
Affinity Capture-MS Homo sapiens
19 Fancd2  
Affinity Capture-MS Mus musculus
20 Kctd13  
Affinity Capture-MS Mus musculus
21 Uchl5 56207
Co-fractionation Mus musculus
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