Gene description for Nck1
Gene name non-catalytic region of tyrosine kinase adaptor protein 1
Gene symbol Nck1
Other names/aliases 6330586M15Rik
D230010O13Rik
Nck
Nck-1
Species Mus musculus
 Database cross references - Nck1
ExoCarta ExoCarta_17973
Vesiclepedia VP_17973
Entrez Gene 17973
UniProt Q99M51  
 Nck1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nck1
Molecular Function
    protein kinase inhibitor activity GO:0004860 ISO
    protein kinase inhibitor activity GO:0004860 ISS
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IPI
    protein-macromolecule adaptor activity GO:0030674 ISO
    receptor tyrosine kinase binding GO:0030971 IBA
    receptor tyrosine kinase binding GO:0030971 IEA
    receptor tyrosine kinase binding GO:0030971 ISO
    signaling adaptor activity GO:0035591 IBA
    signaling adaptor activity GO:0035591 IEA
    ephrin receptor binding GO:0046875 IBA
    ephrin receptor binding GO:0046875 IPI
    eukaryotic initiation factor eIF2 binding GO:0071074 IPI
    eukaryotic initiation factor eIF2 binding GO:0071074 ISO
    molecular condensate scaffold activity GO:0140693 IEA
    molecular condensate scaffold activity GO:0140693 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    substrate-dependent cell migration, cell extension GO:0006930 IGI
    actin filament organization GO:0007015 IMP
    positive regulation of neuron projection development GO:0010976 IMP
    cell migration GO:0016477 IBA
    cell migration GO:0016477 IMP
    lamellipodium assembly GO:0030032 IMP
    regulation of cell migration GO:0030334 IGI
    positive regulation of actin filament polymerization GO:0030838 IGI
    positive regulation of actin filament polymerization GO:0030838 ISO
    negative regulation of peptidyl-serine phosphorylation GO:0033137 ISO
    response to endoplasmic reticulum stress GO:0034976 IGI
    response to endoplasmic reticulum stress GO:0034976 ISO
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 IBA
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 IGI
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 ISO
    positive regulation of T cell proliferation GO:0042102 IEA
    positive regulation of T cell proliferation GO:0042102 ISO
    T cell activation GO:0042110 IEA
    T cell activation GO:0042110 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    negative regulation of insulin receptor signaling pathway GO:0046627 IEA
    negative regulation of insulin receptor signaling pathway GO:0046627 ISO
    ephrin receptor signaling pathway GO:0048013 IBA
    ephrin receptor signaling pathway GO:0048013 IMP
    response to other organism GO:0051707 IMP
    antiviral innate immune response GO:0140374 ISO
    antiviral innate immune response GO:0140374 ISS
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 IBA
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 IGI
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 ISO
    positive regulation of cap-dependent translational initiation GO:1903676 IEA
    positive regulation of cap-dependent translational initiation GO:1903676 ISO
    positive regulation of cap-independent translational initiation GO:1903679 IEA
    positive regulation of cap-independent translational initiation GO:1903679 ISO
    negative regulation of PERK-mediated unfolded protein response GO:1903898 IBA
    negative regulation of PERK-mediated unfolded protein response GO:1903898 IGI
    negative regulation of PERK-mediated unfolded protein response GO:1903898 ISO
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 IGI
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 ISO
Subcellular Localization
    protein phosphatase type 1 complex GO:0000164 IEA
    protein phosphatase type 1 complex GO:0000164 ISO
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    ribosome GO:0005840 IEA
    ribosome GO:0005840 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IDA
    vesicle membrane GO:0012506 IDA
 Experiment description of studies that identified Nck1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nck1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Abl1  
Reconstituted Complex Mus musculus
2 Atp2a3  
Co-fractionation Mus musculus
3 Nedd9  
Co-fractionation Mus musculus
4 Fhl5  
Co-fractionation Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
6 Magi2  
Co-fractionation Mus musculus
7 Kdr  
Affinity Capture-Western Mus musculus
8 Sema7a  
Co-fractionation Mus musculus
9 Tulp1  
Co-fractionation Mus musculus
10 Tnk2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
11 Fyn  
Affinity Capture-Western Mus musculus
12 Scarf2  
Co-fractionation Mus musculus
13 Sepn1  
Co-fractionation Mus musculus
14 Dok1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
15 Psrc1  
Co-fractionation Mus musculus
16 Cbl 12402
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
17 Dok2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here