Gene description for Dlg1
Gene name discs, large homolog 1 (Drosophila)
Gene symbol Dlg1
Other names/aliases B130052P05Rik
Dlgh1
E-dlg/SAP97
SAP-97
SAP97
mKIAA4187
Species Mus musculus
 Database cross references - Dlg1
ExoCarta ExoCarta_13383
Vesiclepedia VP_13383
Entrez Gene 13383
UniProt Q811D0  
 Dlg1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dlg1
Molecular Function
    protein binding GO:0005515 IPI
    protein binding GO:0005515 ISS
    potassium channel regulator activity GO:0015459 IMP
    potassium channel regulator activity GO:0015459 ISO
    kinesin binding GO:0019894 ISO
    kinase binding GO:0019900 ISO
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 ISO
    phosphatase binding GO:0019902 ISO
    phosphatase binding GO:0019902 ISS
    PDZ domain binding GO:0030165 ISO
    ionotropic glutamate receptor binding GO:0035255 IBA
    ionotropic glutamate receptor binding GO:0035255 ISO
    transmembrane transporter binding GO:0044325 IEA
    transmembrane transporter binding GO:0044325 ISO
    molecular adaptor activity GO:0060090 IMP
    L27 domain binding GO:0097016 IEA
    L27 domain binding GO:0097016 ISO
    structural constituent of postsynaptic density GO:0098919 IDA
    structural constituent of postsynaptic density GO:0098919 IEP
    structural constituent of postsynaptic density GO:0098919 IMP
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    ureteric bud development GO:0001657 IMP
    branching involved in ureteric bud morphogenesis GO:0001658 IMP
    immunological synapse formation GO:0001771 IMP
    endothelial cell proliferation GO:0001935 ISO
    endothelial cell proliferation GO:0001935 ISS
    lens development in camera-type eye GO:0002088 IMP
    T cell cytokine production GO:0002369 IMP
    actin filament organization GO:0007015 ISO
    actin filament organization GO:0007015 ISS
    chemical synaptic transmission GO:0007268 IBA
    chemical synaptic transmission GO:0007268 IEA
    nervous system development GO:0007399 IBA
    protein localization GO:0008104 IGI
    protein localization GO:0008104 IMP
    cell population proliferation GO:0008283 IMP
    positive regulation of cell population proliferation GO:0008284 IMP
    regulation of cell shape GO:0008360 IEA
    regulation of cell shape GO:0008360 ISO
    positive regulation of gene expression GO:0010628 ISO
    epithelial structure maintenance GO:0010669 ISS
    actin filament polymerization GO:0030041 IMP
    peristalsis GO:0030432 IMP
    positive regulation of actin filament polymerization GO:0030838 IMP
    cortical actin cytoskeleton organization GO:0030866 ISO
    cortical actin cytoskeleton organization GO:0030866 ISS
    astral microtubule organization GO:0030953 IEA
    astral microtubule organization GO:0030953 ISO
    protein-containing complex localization GO:0031503 ISO
    membrane raft organization GO:0031579 IMP
    regulation of myelination GO:0031641 IMP
    activation of protein kinase activity GO:0032147 IMP
    regulation of protein localization GO:0032880 ISO
    protein localization to synapse GO:0035418 IBA
    positive regulation of multicellular organism growth GO:0040018 TAS
    T cell proliferation GO:0042098 IMP
    T cell activation GO:0042110 IMP
    negative regulation of T cell proliferation GO:0042130 IMP
    regulation of membrane potential GO:0042391 IGI
    regulation of membrane potential GO:0042391 ISO
    amyloid precursor protein metabolic process GO:0042982 IGI
    receptor clustering GO:0043113 IBA
    positive regulation of potassium ion transport GO:0043268 IEA
    positive regulation of potassium ion transport GO:0043268 ISO
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IMP
    cortical microtubule organization GO:0043622 IEA
    cortical microtubule organization GO:0043622 ISO
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IBA
    reproductive structure development GO:0048608 IMP
    positive regulation of developmental growth GO:0048639 TAS
    embryonic skeletal system morphogenesis GO:0048704 IMP
    tissue morphogenesis GO:0048729 IMP
    smooth muscle tissue development GO:0048745 IMP
    negative regulation of epithelial cell proliferation GO:0050680 IMP
    establishment of centrosome localization GO:0051660 IEA
    establishment of centrosome localization GO:0051660 ISO
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IMP
    hard palate development GO:0060022 IMP
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    bicellular tight junction assembly GO:0070830 IEA
    bicellular tight junction assembly GO:0070830 ISO
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    receptor localization to synapse GO:0097120 IBA
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion GO:0098609 ISO
    cell-cell adhesion GO:0098609 ISS
    regulation of ventricular cardiac muscle cell action potential GO:0098911 IMP
    membrane repolarization during ventricular cardiac muscle cell action potential GO:0098915 IMP
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IBA
    maintenance of postsynaptic density structure GO:0099562 IEA
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IDA
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IEP
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IMP
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 ISO
    regulation of protein localization to synapse GO:1902473 IDA
    regulation of protein localization to synapse GO:1902473 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 IEA
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    regulation of potassium ion import GO:1903286 IMP
    negative regulation of p38MAPK cascade GO:1903753 IEA
    negative regulation of p38MAPK cascade GO:1903753 ISO
    regulation of potassium ion export across plasma membrane GO:1903764 IMP
    cellular response to brain-derived neurotrophic factor stimulus GO:1990416 ISO
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 ISO
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 ISS
Subcellular Localization
    immunological synapse GO:0001772 IDA
    immunological synapse GO:0001772 ISO
    basement membrane GO:0005604 IDA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    microvillus GO:0005902 ISO
    cell-cell junction GO:0005911 ISO
    adherens junction GO:0005912 TAS
    bicellular tight junction GO:0005923 IEA
    bicellular tight junction GO:0005923 ISO
    cytoplasmic side of plasma membrane GO:0009898 IEA
    cytoplasmic side of plasma membrane GO:0009898 ISO
    basal plasma membrane GO:0009925 ISO
    postsynaptic density GO:0014069 IDA
    postsynaptic density GO:0014069 ISO
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    basolateral plasma membrane GO:0016323 ISS
    basolateral plasma membrane GO:0016323 TAS
    apical plasma membrane GO:0016324 IEA
    lateral plasma membrane GO:0016328 IDA
    lateral plasma membrane GO:0016328 ISO
    cell junction GO:0030054 IDA
    cell junction GO:0030054 ISO
    T-tubule GO:0030315 ISO
    dendrite GO:0030425 ISO
    synaptic vesicle membrane GO:0030672 ISO
    cell projection membrane GO:0031253 IDA
    neuromuscular junction GO:0031594 IBA
    neuromuscular junction GO:0031594 IDA
    node of Ranvier GO:0033268 IDA
    paranode region of axon GO:0033270 ISO
    myelin sheath abaxonal region GO:0035748 IDA
    sarcolemma GO:0042383 IEA
    presynaptic membrane GO:0042734 ISO
    neuron projection GO:0043005 IBA
    neuronal cell body GO:0043025 ISO
    lateral loop GO:0043219 IDA
    membrane raft GO:0045121 IDA
    synapse GO:0045202 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    MPP7-DLG1-LIN7 complex GO:0097025 IEA
    MPP7-DLG1-LIN7 complex GO:0097025 ISO
    synaptic membrane GO:0097060 IDA
    synaptic membrane GO:0097060 ISO
    postsynaptic density membrane GO:0098839 IBA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
    postsynaptic density, intracellular component GO:0099092 ISO
 Experiment description of studies that identified Dlg1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dlg1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mapk14  
Affinity Capture-Western Mus musculus
2 NET1 10276
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
3 Adam10 11487
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
4 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
5 Fmr1  
Affinity Capture-MS Mus musculus
6 Nrcam  
Affinity Capture-Western Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Lin7c 22343
Affinity Capture-MS Mus musculus
9 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
10 Tnik  
Affinity Capture-MS Mus musculus
11 Hcn1  
Affinity Capture-MS Mus musculus
12 Zap70 22637
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
13 Cnksr2  
Affinity Capture-MS Mus musculus
14 Agap2  
Affinity Capture-MS Mus musculus
15 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
16 Lck 16818
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
17 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
18 Grin1  
Affinity Capture-MS Mus musculus
19 Cbl 12402
Affinity Capture-Western Mus musculus
20 Lin7a  
Co-fractionation Mus musculus
21 Was 22376
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
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