Gene description for Sh3kbp1
Gene name SH3-domain kinase binding protein 1
Gene symbol Sh3kbp1
Other names/aliases 1200007H22Rik
1700125L08Rik
5830464D22Rik
AI447724
IN85
Ruk
Seta
Species Mus musculus
 Database cross references - Sh3kbp1
ExoCarta ExoCarta_58194
Vesiclepedia VP_58194
Entrez Gene 58194
UniProt Q8R550  
 Sh3kbp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sh3kbp1
Molecular Function
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    molecular adaptor activity GO:0060090 ISO
Biological Process
    apoptotic process GO:0006915 IEA
    cytoskeleton organization GO:0007010 ISO
    cytoskeleton organization GO:0007010 ISS
    actin filament organization GO:0007015 IBA
    regulation of cell shape GO:0008360 ISO
    regulation of cell shape GO:0008360 ISS
    cell migration GO:0016477 IBA
    cell migration GO:0016477 ISO
    cell migration GO:0016477 ISS
    positive regulation of B cell activation GO:0050871 IEA
    positive regulation of B cell activation GO:0050871 ISO
    ubiquitin-dependent endocytosis GO:0070086 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    cell-cell junction GO:0005911 IDA
    focal adhesion GO:0005925 IEA
    postsynaptic density GO:0014069 IEA
    endocytic vesicle GO:0030139 IDA
    cytoplasmic vesicle membrane GO:0030659 IEA
    neuron projection GO:0043005 IEA
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified Sh3kbp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sh3kbp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tnik  
Affinity Capture-MS Mus musculus
2 Hcn1  
Affinity Capture-MS Mus musculus
3 Sh3gl2  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
4 Atxn2 20239
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
5 Grb2 14784
Co-fractionation Mus musculus
6 Synj2  
Co-fractionation Mus musculus
7 Dlg4  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
8 Drd2  
Affinity Capture-Western Mus musculus
9 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
10 Dnm1 13429
Affinity Capture-Western Mus musculus
11 Capza1 12340
Co-fractionation Mus musculus
12 Kctd13  
Affinity Capture-MS Mus musculus
13 Rab7 19349
Co-fractionation Mus musculus
14 Dnm2 13430
Co-fractionation Mus musculus
15 Inpp5d 16331
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
16 Sept11  
Co-fractionation Mus musculus
17 Spry2  
Affinity Capture-Western Mus musculus
18 Sh3glb2  
Co-fractionation Mus musculus
19 Rab5a 271457
Co-fractionation Mus musculus
20 Tubgcp2  
Co-fractionation Mus musculus
21 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 Sos1  
Co-fractionation Mus musculus
23 Capza2 12343
Co-fractionation Mus musculus
24 BMPR1B 658
Affinity Capture-Luminescence Homo sapiens
25 Cbl 12402
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
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