Experiment description of studies that identified Capza1 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

3 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Capza1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Psmg1 56088 Co-fractionation Mus musculus 2 DCTN3 11258 Affinity Capture-MS Homo sapiens 3 Strap 20901 Co-fractionation Mus musculus 4 E430025E21Rik   Co-fractionation Mus musculus 5 KIAA0196 9897 Affinity Capture-MS Homo sapiens 6 DCTN6 10671 Affinity Capture-MS Homo sapiens 7 DCTN4 51164 Affinity Capture-MS Homo sapiens 8 Uchl5 56207 Co-fractionation Mus musculus 9 Ap3b1   Co-fractionation Mus musculus 10 CD2AP 23607 Affinity Capture-MS Homo sapiens 11 Kctd13   Affinity Capture-MS Mus musculus 12 FAM21A 387680 Affinity Capture-MS Homo sapiens 13 Fkbp15   Co-fractionation Mus musculus 14 ACTR1B 10120 Affinity Capture-MS Homo sapiens 15 DCTN2 10540 Affinity Capture-MS Homo sapiens 16 Homer2   Affinity Capture-Western Mus musculus 17 Bag3   Affinity Capture-Western Mus musculus 18 Ywhae 22627 Affinity Capture-MS Mus musculus 19 LRRC16A 55604 Affinity Capture-MS Homo sapiens 20 DCTN1 1639 Affinity Capture-MS Homo sapiens 21 TNKS1BP1 85456 Affinity Capture-MS Homo sapiens 22 Mettl21e   Affinity Capture-MS Mus musculus 23 Sgta 52551 Co-fractionation Mus musculus 24 Tmem173   Proximity Label-MS Mus musculus 25 CNOT1 23019 Affinity Capture-MS Homo sapiens 26 Sh3kbp1 58194 Co-fractionation Mus musculus 27 Nanog   Affinity Capture-MS Mus musculus 28 HACE1 57531 Affinity Capture-MS Homo sapiens 29 FAM21C 253725 Affinity Capture-MS Homo sapiens 30 Fam21   Co-fractionation Mus musculus 31 ACTR1A 10121 Affinity Capture-MS Homo sapiens 32 SH3KBP1 30011 Affinity Capture-MS Homo sapiens 33 Eed   Affinity Capture-MS Mus musculus 34 MTCL1 23255 Affinity Capture-MS Homo sapiens 35 ACTR10 55860 Affinity Capture-MS Homo sapiens 36 KIAA1033 23325 Affinity Capture-MS Homo sapiens 37 Fancd2   Affinity Capture-MS Mus musculus View the network

Pathways in which Capza1 is involved

No pathways found