Gene description for Dnm1
Gene name dynamin 1
Gene symbol Dnm1
Other names/aliases AI838169
Dnm
Ftfl
mKIAA4093
Species Mus musculus
 Database cross references - Dnm1
ExoCarta ExoCarta_13429
Vesiclepedia VP_13429
Entrez Gene 13429
UniProt P39053  
 Dnm1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dnm1
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISO
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISS
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IEA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
    microtubule binding GO:0008017 IBA
    SH3 domain binding GO:0017124 ISO
    GDP binding GO:0019003 IDA
    protein kinase binding GO:0019901 IPI
    D2 dopamine receptor binding GO:0031749 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    protein-containing complex binding GO:0044877 ISO
    nitric-oxide synthase binding GO:0050998 ISO
    protein serine/threonine kinase binding GO:0120283 ISO
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    G protein-coupled receptor internalization GO:0002031 ISO
    endocytosis GO:0006897 ISO
    receptor-mediated endocytosis GO:0006898 ISO
    receptor-mediated endocytosis GO:0006898 ISS
    endosome organization GO:0007032 IEA
    endosome organization GO:0007032 ISO
    synaptic vesicle budding from presynaptic endocytic zone membrane GO:0016185 IBA
    receptor internalization GO:0031623 IBA
    receptor internalization GO:0031623 ISO
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    protein homooligomerization GO:0051260 ISO
    protein homotetramerization GO:0051289 ISO
    protein homotetramerization GO:0051289 ISS
    regulation of vesicle size GO:0097494 IMP
    clathrin coat assembly involved in endocytosis GO:0099049 IMP
    vesicle scission GO:0099050 ISO
    vesicle scission GO:0099050 ISS
    regulation of synaptic vesicle endocytosis GO:1900242 ISO
    positive regulation of synaptic vesicle endocytosis GO:1900244 ISO
    positive regulation of synaptic vesicle recycling GO:1903423 ISO
Subcellular Localization
    photoreceptor inner segment GO:0001917 IDA
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 ISO
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
    plasma membrane GO:0005886 IBA
    clathrin-coated pit GO:0005905 ISO
    clathrin-coated pit GO:0005905 ISS
    synaptic vesicle GO:0008021 ISO
    membrane coat GO:0030117 IDA
    clathrin-coated vesicle GO:0030136 ISO
    endocytic vesicle GO:0030139 ISO
    endocytic vesicle GO:0030139 ISS
    protein-containing complex GO:0032991 ISO
    chromaffin granule GO:0042583 IEA
    cell projection GO:0042995 IEA
    varicosity GO:0043196 ISO
    myelin sheath GO:0043209 HDA
    synapse GO:0045202 IBA
    photoreceptor ribbon synapse GO:0098684 IDA
    presynapse GO:0098793 ISO
    presynapse GO:0098793 ISS
    presynaptic endocytic zone membrane GO:0098835 IDA
    presynaptic endocytic zone membrane GO:0098835 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Dnm1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dnm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tnik  
Affinity Capture-MS Mus musculus
2 Sh3gl2  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
3 Cyfip2 76884
Affinity Capture-MS Mus musculus
4 Kif5a  
Affinity Capture-Western Mus musculus
5 Prnp 19122
Affinity Capture-MS Mus musculus
6 Pfn2  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
7 Drd2  
Affinity Capture-Western Mus musculus
8 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
9 Dnm1 13429
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
10 Syngap1  
Affinity Capture-MS Mus musculus
11 Fmr1  
Affinity Capture-MS Mus musculus
12 Kcna3  
Affinity Capture-MS Mus musculus
13 Kctd13  
Affinity Capture-MS Mus musculus
14 MTNR1A  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
15 Pfn1 18643
Affinity Capture-Western Mus musculus
16 Tuba1a 22142
Affinity Capture-Western Mus musculus
17 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
18 Synj1 104015
Co-fractionation Mus musculus
19 Kcnma1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
20 Nckap1 50884
Affinity Capture-MS Mus musculus
21 AMPH 273
Reconstituted Complex Homo sapiens
22 HTT 3064
Affinity Capture-MS Homo sapiens
23 Nck2 17974
Two-hybrid Mus musculus
24 Grin2b  
Affinity Capture-Western Mus musculus
25 Cyfip1 20430
Affinity Capture-MS Mus musculus
26 Itsn1 16443
Affinity Capture-Western Mus musculus
27 Agap2  
Affinity Capture-MS Mus musculus
28 LDLRAD3  
Reconstituted Complex Homo sapiens
29 Runx2  
Affinity Capture-MS Mus musculus
30 Sh3kbp1 58194
Affinity Capture-Western Mus musculus
31 Grin1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
32 Sh3gl1 20405
Affinity Capture-Western Mus musculus
33 Vamp2  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
34 Snrpa1 68981
Co-fractionation Mus musculus
35 Atg5  
Affinity Capture-MS Mus musculus
36 Cltc 67300
Affinity Capture-Western Mus musculus
37 Syn2  
Co-fractionation Mus musculus
38 Fancd2  
Affinity Capture-MS Mus musculus
39 Homer1  
Affinity Capture-MS Mus musculus
40 Kif2a 16563
Affinity Capture-Western Mus musculus
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