Gene description for Nckap1
Gene name NCK-associated protein 1
Gene symbol Nckap1
Other names/aliases C79304
H19
Hem-2
Hem2
Nap1
mKIAA0587
mh19
p125Nap1
Species Mus musculus
 Database cross references - Nckap1
ExoCarta ExoCarta_50884
Vesiclepedia VP_50884
Entrez Gene 50884
UniProt P28660  
 Nckap1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nckap1
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 ISO
Biological Process
    cell morphogenesis GO:0000902 IBA
    in utero embryonic development GO:0001701 IMP
    somitogenesis GO:0001756 IMP
    neural tube closure GO:0001843 IMP
    zygotic determination of anterior/posterior axis, embryo GO:0007354 IMP
    endoderm development GO:0007492 IMP
    mesodermal cell migration GO:0008078 IMP
    embryonic body morphogenesis GO:0010172 IMP
    positive regulation of lamellipodium assembly GO:0010592 IDA
    positive regulation of lamellipodium assembly GO:0010592 ISO
    cell migration GO:0016477 IBA
    Rac protein signal transduction GO:0016601 ISO
    cell projection assembly GO:0030031 IBA
    lamellipodium assembly GO:0030032 IDA
    lamellipodium assembly GO:0030032 IMP
    positive regulation of actin filament polymerization GO:0030838 IDA
    cortical actin cytoskeleton organization GO:0030866 IBA
    notochord development GO:0030903 IMP
    establishment or maintenance of actin cytoskeleton polarity GO:0030950 IMP
    regulation of protein localization GO:0032880 IMP
    embryonic heart tube development GO:0035050 IMP
    cell migration involved in gastrulation GO:0042074 IMP
    basal protein localization GO:0045175 IDA
    apical protein localization GO:0045176 IDA
    paraxial mesoderm development GO:0048339 IMP
    paraxial mesoderm morphogenesis GO:0048340 IMP
    notochord morphogenesis GO:0048570 IMP
    embryonic foregut morphogenesis GO:0048617 IMP
    neuron projection morphogenesis GO:0048812 IBA
    protein stabilization GO:0050821 IMP
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 ISO
Subcellular Localization
    ruffle GO:0001726 IDA
    cytoplasm GO:0005737 IEA
    lamellipodium GO:0030027 IDA
    SCAR complex GO:0031209 IBA
    SCAR complex GO:0031209 ISO
    lamellipodium membrane GO:0031258 IEA
    filamentous actin GO:0031941 IDA
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IEP
 Experiment description of studies that identified Nckap1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nckap1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Wasf1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
2 Camk2a  
Affinity Capture-MS Mus musculus
3 Tnik  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
4 Abi2  
Affinity Capture-MS Mus musculus
5 Myo16  
Affinity Capture-Western Mus musculus
6 Nyap2  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
7 Pfn2  
Affinity Capture-MS Mus musculus
8 Pik3r1  
Affinity Capture-Western Mus musculus
9 Dpysl2 12934
Affinity Capture-MS Mus musculus
10 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
11 Nefm 18040
Affinity Capture-MS Mus musculus
12 Dnm1 13429
Affinity Capture-MS Mus musculus
13 Tsc1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
14 Fmr1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
15 Epb4.1l1 13821
Affinity Capture-MS Mus musculus
16 Kctd13  
Affinity Capture-MS Mus musculus
17 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
18 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
19 Pex5l  
Affinity Capture-MS Mus musculus
20 Chaf1a  
Affinity Capture-MS Mus musculus
21 Sgip1  
Affinity Capture-MS Mus musculus
22 Wasf3  
Co-fractionation Mus musculus
23 Baiap2 108100
Affinity Capture-MS Mus musculus
24 Synpo  
Affinity Capture-MS Mus musculus
25 Sptbn1 20742
Affinity Capture-MS Mus musculus
26 Cyfip2 76884
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
27 Cyfip1 20430
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
28 Abi1 11308
Affinity Capture-MS Mus musculus
29 Epb4.1l3 13823
Affinity Capture-MS Mus musculus
30 Agap2  
Affinity Capture-MS Mus musculus
31 Syn2  
Co-fractionation Mus musculus
32 Wipf2  
Co-fractionation Mus musculus
33 Snap25 20614
Affinity Capture-MS Mus musculus
34 Nefl  
Affinity Capture-MS Mus musculus
35 Nyap1  
Affinity Capture-Western Mus musculus
36 Ap2a1 11771
Affinity Capture-MS Mus musculus
37 Sptbn2  
Affinity Capture-MS Mus musculus
38 Eed  
Affinity Capture-MS Mus musculus
39 Mapt  
Affinity Capture-MS Mus musculus
40 Rac1 19353
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
41 Sptan1 20740
Affinity Capture-MS Mus musculus
42 Gsk3b  
Affinity Capture-MS Mus musculus
43 Wasf2 242687
Co-fractionation Mus musculus
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