Experiment description of studies that identified Sptan1 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 210 MISEV standards ✔ EM Biophysical techniques ✔ CD81|FLOT1 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23260141     Organism Mus musculus Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL." Journal name Cell Publication year 2012 Sample Fibroblasts Sample name Normal-Fibroblasts (L cells) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Sptan1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Kctd13   Affinity Capture-MS Mus musculus 2 Ubc   Reconstituted Complex Mus musculus 3 SNCA 6622 Affinity Capture-MS Homo sapiens 4 Cnksr2   Affinity Capture-MS Mus musculus 5 Cyfip2 76884 Affinity Capture-MS Mus musculus 6 Mycbp2 105689 Affinity Capture-MS Mus musculus 7 Tnik   Affinity Capture-MS Mus musculus 8 Kalrn   Affinity Capture-MS Mus musculus 9 Hspb2 69253 Affinity Capture-MS Mus musculus 10 Htt   Affinity Capture-MS Mus musculus 11 Dlgap1   Affinity Capture-MS Mus musculus Affinity Capture-MS Mus musculus 12 Ncam1 17967 Affinity Capture-Western Mus musculus 13 Cebpa   Affinity Capture-MS Mus musculus 14 YWHAB 7529 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 15 Shank3   Affinity Capture-MS Mus musculus Affinity Capture-MS Mus musculus 16 CSNK2A1 1457 Biochemical Activity Homo sapiens 17 Iqcb1   Affinity Capture-MS Mus musculus 18 Ywhaz 22631 Affinity Capture-MS Mus musculus 19 Nckap1 50884 Affinity Capture-MS Mus musculus 20 Nphp4   Affinity Capture-MS Mus musculus 21 Pde4dip   Affinity Capture-MS Mus musculus 22 Cyfip1 20430 Affinity Capture-MS Mus musculus 23 Ywhab 54401 Affinity Capture-Western Mus musculus Reconstituted Complex Mus musculus Reconstituted Complex Mus musculus 24 Agap2   Affinity Capture-MS Mus musculus 25 Casp3 12367 Biochemical Activity Mus musculus 26 Pex5l   Affinity Capture-MS Mus musculus 27 SH3KBP1 30011 Affinity Capture-MS Homo sapiens 28 Eed   Affinity Capture-MS Mus musculus 29 Fancd2   Affinity Capture-MS Mus musculus 30 Homer1   Affinity Capture-MS Mus musculus View the network

Pathways in which Sptan1 is involved

No pathways found