Gene description for Casp3
Gene name caspase 3
Gene symbol Casp3
Other names/aliases A830040C14Rik
AC-3
Apopain
CASP-3
CC3
CPP-32
CPP32
Caspase-3
Lice
SCA-1
Yama
mldy
Species Mus musculus
 Database cross references - Casp3
ExoCarta ExoCarta_12367
Vesiclepedia VP_12367
Entrez Gene 12367
UniProt P70677  
 Casp3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Casp3
Molecular Function
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    endopeptidase activity GO:0004175 IDA
    aspartic-type endopeptidase activity GO:0004190 IDA
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 IDA
    cysteine-type endopeptidase activity GO:0004197 ISO
    cysteine-type endopeptidase activity GO:0004197 ISS
    cyclin-dependent protein serine/threonine kinase inhibitor activity GO:0004861 IMP
    death receptor binding GO:0005123 IEA
    death receptor binding GO:0005123 ISO
    protein binding GO:0005515 IPI
    enzyme activator activity GO:0008047 IBA
    peptidase activity GO:0008233 IDA
    peptidase activity GO:0008233 IMP
    peptidase activity GO:0008233 ISO
    peptidase activity GO:0008233 RCA
    cysteine-type peptidase activity GO:0008234 IDA
    cysteine-type peptidase activity GO:0008234 ISO
    phospholipase A2 activator activity GO:0016005 IEA
    phospholipase A2 activator activity GO:0016005 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    luteolysis GO:0001554 IEA
    response to hypoxia GO:0001666 IEA
    B cell homeostasis GO:0001782 IMP
    negative regulation of cytokine production GO:0001818 IEA
    negative regulation of cytokine production GO:0001818 ISO
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 ISO
    proteolysis GO:0006508 RCA
    apoptotic process GO:0006915 IBA
    apoptotic process GO:0006915 IMP
    apoptotic process GO:0006915 ISO
    DNA damage response GO:0006974 IDA
    axonal fasciculation GO:0007413 IEA
    axonal fasciculation GO:0007413 ISO
    heart development GO:0007507 IGI
    sensory perception of sound GO:0007605 IMP
    learning or memory GO:0007611 IEA
    learning or memory GO:0007611 ISO
    intrinsic apoptotic signaling pathway in response to osmotic stress GO:0008627 IDA
    response to xenobiotic stimulus GO:0009410 IEA
    response to UV GO:0009411 IDA
    response to UV GO:0009411 IGI
    response to wounding GO:0009611 IDA
    response to glucose GO:0009749 IEA
    response to glucose GO:0009749 ISO
    response to X-ray GO:0010165 IEA
    protein processing GO:0016485 IDA
    protein processing GO:0016485 ISO
    hippocampus development GO:0021766 IEA
    protein catabolic process GO:0030163 IEA
    protein catabolic process GO:0030163 ISO
    neuron differentiation GO:0030182 IBA
    keratinocyte differentiation GO:0030216 IBA
    keratinocyte differentiation GO:0030216 IMP
    erythrocyte differentiation GO:0030218 IBA
    erythrocyte differentiation GO:0030218 IEA
    erythrocyte differentiation GO:0030218 ISO
    negative regulation of B cell proliferation GO:0030889 IMP
    regulation of protein stability GO:0031647 ISO
    regulation of protein stability GO:0031647 ISS
    response to cobalt ion GO:0032025 IEA
    response to estradiol GO:0032355 IEA
    response to lipopolysaccharide GO:0032496 IEA
    glial cell apoptotic process GO:0034349 IMP
    response to nicotine GO:0035094 IEA
    intracellular signal transduction GO:0035556 ISO
    response to hydrogen peroxide GO:0042542 IEA
    response to hydrogen peroxide GO:0042542 ISO
    T cell homeostasis GO:0043029 IMP
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    response to amino acid GO:0043200 IEA
    positive regulation of neuron apoptotic process GO:0043525 IBA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    positive regulation of neuron apoptotic process GO:0043525 ISO
    fibroblast apoptotic process GO:0044346 IDA
    fibroblast apoptotic process GO:0044346 IMP
    cell fate commitment GO:0045165 IMP
    negative regulation of cyclin-dependent protein serine/threonine kinase activity GO:0045736 IMP
    negative regulation of cell cycle GO:0045786 IMP
    negative regulation of activated T cell proliferation GO:0046007 IMP
    neurotrophin TRK receptor signaling pathway GO:0048011 ISO
    striated muscle cell differentiation GO:0051146 IEA
    response to glucocorticoid GO:0051384 IEA
    neuron apoptotic process GO:0051402 IDA
    neuron apoptotic process GO:0051402 IGI
    neuron apoptotic process GO:0051402 IMP
    protein maturation GO:0051604 ISO
    anterior neural tube closure GO:0061713 IMP
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 NAS
    pyroptotic inflammatory response GO:0070269 IEA
    pyroptotic inflammatory response GO:0070269 ISO
    leukocyte apoptotic process GO:0071887 IEA
    cellular response to staurosporine GO:0072734 IEA
    cellular response to staurosporine GO:0072734 ISO
    intrinsic apoptotic signaling pathway GO:0097193 ISO
    execution phase of apoptosis GO:0097194 IBA
    execution phase of apoptosis GO:0097194 IDA
    execution phase of apoptosis GO:0097194 IGI
    execution phase of apoptosis GO:0097194 IMP
    execution phase of apoptosis GO:0097194 IMP
    execution phase of apoptosis GO:0097194 ISO
    regulation of synaptic vesicle cycle GO:0098693 IDA
    regulation of synaptic vesicle cycle GO:0098693 IMP
    positive regulation of pyroptotic inflammatory response GO:0140639 IEA
    positive regulation of pyroptotic inflammatory response GO:0140639 ISO
    positive regulation of amyloid-beta formation GO:1902004 ISO
    positive regulation of amyloid-beta formation GO:1902004 ISS
    epithelial cell apoptotic process GO:1904019 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    postsynaptic density GO:0014069 IDA
    postsynaptic density GO:0014069 IEP
    death-inducing signaling complex GO:0031264 IBA
    death-inducing signaling complex GO:0031264 IEA
    death-inducing signaling complex GO:0031264 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
 Experiment description of studies that identified Casp3 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Casp3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gzmb  
Biochemical Activity Mus musculus
2 Dffb  
Biochemical Activity Mus musculus
3 GZMB  
Biochemical Activity Homo sapiens
4 Tmem173  
Proximity Label-MS Mus musculus
5 Parp1 11545
Biochemical Activity Mus musculus
6 Bid  
Biochemical Activity Mus musculus
7 Sars2  
Co-fractionation Mus musculus
8 Psme3 19192
Biochemical Activity Mus musculus
9 Sptan1 20740
Biochemical Activity Mus musculus
10 Ctnnb1 12387
Biochemical Activity Mus musculus
11 Arhgdia 192662
Biochemical Activity Mus musculus
12 Birc3  
Biochemical Activity Mus musculus
13 Rock1  
Biochemical Activity Mus musculus
14 Slc1a2  
Biochemical Activity Mus musculus
15 FEM1B 10116
Biochemical Activity Homo sapiens
16 Ywhae 22627
Affinity Capture-MS Mus musculus
17 Ube4b  
Co-fractionation Mus musculus
18 Dffa  
Biochemical Activity Mus musculus
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