Gene description for Arhgdia
Gene name Rho GDP dissociation inhibitor (GDI) alpha
Gene symbol Arhgdia
Other names/aliases 5330430M07Rik
Gdi-1
Rho-GDI
RhoDGI
RhoGDI-1
Species Mus musculus
 Database cross references - Arhgdia
ExoCarta ExoCarta_192662
Vesiclepedia VP_192662
Entrez Gene 192662
UniProt Q99PT1  
 Arhgdia identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arhgdia
Molecular Function
    Rho GDP-dissociation inhibitor activity GO:0005094 IBA
    Rho GDP-dissociation inhibitor activity GO:0005094 IDA
    GTPase activator activity GO:0005096 IEA
    fatty acid binding GO:0005504 ISO
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 ISO
    small GTPase binding GO:0031267 ISO
    Hsp90 protein binding GO:0051879 ISO
Biological Process
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 IDA
    regulation of cell shape GO:0008360 ISO
    negative regulation of cell migration GO:0030336 ISO
    regulation of protein localization GO:0032880 IDA
    regulation of Rho protein signal transduction GO:0035023 IEA
    regulation of Rho protein signal transduction GO:0035023 ISO
    cellular response to mechanical stimulus GO:0071260 ISO
    semaphorin-plexin signaling pathway GO:0071526 IMP
    regulation of synaptic vesicle cycle GO:0098693 IDA
    regulation of synaptic vesicle cycle GO:0098693 IMP
Subcellular Localization
    immunological synapse GO:0001772 IDA
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 IBA
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    synapse GO:0045202 ISO
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
 Experiment description of studies that identified Arhgdia in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arhgdia
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF128  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
2 Ikbkg  
Affinity Capture-Western Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 MAPT  
Reconstituted Complex Homo sapiens
6 Traf3  
Proximity Label-MS Mus musculus
7 Rac1 19353
Affinity Capture-Western Mus musculus
8 Rhoa 11848
Co-fractionation Mus musculus
9 Casp7  
Biochemical Activity Mus musculus
10 Casp3 12367
Biochemical Activity Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
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