Gene description for Rhoa
Gene name ras homolog gene family, member A
Gene symbol Rhoa
Other names/aliases Arha
Arha1
Arha2
Species Mus musculus
 Database cross references - Rhoa
ExoCarta ExoCarta_11848
Vesiclepedia VP_11848
Entrez Gene 11848
UniProt Q9QUI0  
 Rhoa identified in exosomes derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rhoa
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    myosin binding GO:0017022 IEA
    myosin binding GO:0017022 ISO
    GDP binding GO:0019003 ISO
    protein kinase binding GO:0019901 IBA
    protein domain specific binding GO:0019904 ISO
    Rho GDP-dissociation inhibitor binding GO:0051022 ISO
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    cell morphogenesis GO:0000902 IGI
    neuron migration GO:0001764 IMP
    kidney development GO:0001822 IGI
    angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure GO:0001998 IMP
    alpha-beta T cell lineage commitment GO:0002363 IMP
    regulation of systemic arterial blood pressure by endothelin GO:0003100 IMP
    aortic valve formation GO:0003189 IEA
    aortic valve formation GO:0003189 ISO
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    cytoskeleton organization GO:0007010 IMP
    microtubule depolymerization GO:0007019 ISO
    cell adhesion GO:0007155 IMP
    cell-matrix adhesion GO:0007160 IDA
    integrin-mediated signaling pathway GO:0007229 TAS
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 IMP
    Rho protein signal transduction GO:0007266 ISO
    Rho protein signal transduction GO:0007266 ISO
    Rho protein signal transduction GO:0007266 ISS
    Rho protein signal transduction GO:0007266 TAS
    skeletal muscle tissue development GO:0007519 IDA
    response to xenobiotic stimulus GO:0009410 ISO
    negative regulation of cell-substrate adhesion GO:0010812 IMP
    regulation of neuron projection development GO:0010975 IDA
    negative regulation of neuron projection development GO:0010977 ISO
    cell migration GO:0016477 IBA
    cell migration GO:0016477 ISO
    cell migration GO:0016477 ISS
    cerebral cortex cell migration GO:0021795 IMP
    forebrain radial glial cell differentiation GO:0021861 IMP
    actin cytoskeleton organization GO:0030036 IDA
    actin cytoskeleton organization GO:0030036 ISO
    actin cytoskeleton organization GO:0030036 ISS
    cell differentiation GO:0030154 IDA
    positive regulation of cell growth GO:0030307 ISO
    regulation of cell migration GO:0030334 ISO
    regulation of cell migration GO:0030334 ISS
    androgen receptor signaling pathway GO:0030521 IDA
    positive regulation of actin filament polymerization GO:0030838 ISO
    stress-activated protein kinase signaling cascade GO:0031098 ISO
    cytoplasmic microtubule organization GO:0031122 ISO
    cytoplasmic microtubule organization GO:0031122 ISS
    positive regulation of cytokinesis GO:0032467 ISO
    positive regulation of cytokinesis GO:0032467 ISS
    regulation of actin cytoskeleton organization GO:0032956 IBA
    regulation of actin cytoskeleton organization GO:0032956 IMP
    regulation of actin cytoskeleton organization GO:0032956 ISO
    negative regulation of intracellular steroid hormone receptor signaling pathway GO:0033144 IDA
    regulation of osteoblast proliferation GO:0033688 IMP
    cell junction assembly GO:0034329 ISO
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    substrate adhesion-dependent cell spreading GO:0034446 ISS
    Roundabout signaling pathway GO:0035385 ISO
    Roundabout signaling pathway GO:0035385 ISS
    cleavage furrow formation GO:0036089 ISO
    cleavage furrow formation GO:0036089 ISS
    apolipoprotein A-I-mediated signaling pathway GO:0038027 IEA
    apolipoprotein A-I-mediated signaling pathway GO:0038027 ISO
    odontogenesis GO:0042476 IEP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISS
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 ISO
    stress fiber assembly GO:0043149 IBA
    stress fiber assembly GO:0043149 IDA
    stress fiber assembly GO:0043149 ISO
    apical junction assembly GO:0043297 IDA
    apical junction assembly GO:0043297 ISO
    beta selection GO:0043366 IMP
    positive regulation of neuron apoptotic process GO:0043525 ISO
    endothelial cell migration GO:0043542 ISO
    ossification involved in bone maturation GO:0043931 IMP
    wound healing, spreading of cells GO:0044319 ISS
    establishment of epithelial cell apical/basal polarity GO:0045198 ISO
    establishment of epithelial cell apical/basal polarity GO:0045198 ISS
    negative regulation of neuron differentiation GO:0045665 ISO
    positive regulation of neuron differentiation GO:0045666 ISO
    negative regulation of cell size GO:0045792 IEA
    negative regulation of cell size GO:0045792 ISO
    positive regulation of smooth muscle contraction GO:0045987 ISO
    GTP metabolic process GO:0046039 ISO
    positive regulation of alpha-beta T cell differentiation GO:0046638 IMP
    regulation of dendrite development GO:0050773 ISO
    negative chemotaxis GO:0050919 IEA
    negative chemotaxis GO:0050919 ISO
    positive regulation of stress fiber assembly GO:0051496 IDA
    positive regulation of stress fiber assembly GO:0051496 ISO
    positive regulation of stress fiber assembly GO:0051496 ISO
    regulation of calcium ion transport GO:0051924 ISO
    negative regulation of synapse assembly GO:0051964 ISO
    positive regulation of lipase activity GO:0060193 ISO
    bone trabecula morphogenesis GO:0061430 IMP
    regulation of microtubule cytoskeleton organization GO:0070507 IMP
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to cytokine stimulus GO:0071345 ISO
    semaphorin-plexin signaling pathway GO:0071526 IMP
    positive regulation of podosome assembly GO:0071803 IGI
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISO
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISS
    negative regulation of cell migration involved in sprouting angiogenesis GO:0090051 ISO
    mitotic spindle assembly GO:0090307 IEA
    mitotic spindle assembly GO:0090307 ISO
    negative regulation of oxidative phosphorylation GO:0090324 IMP
    motor neuron apoptotic process GO:0097049 IMP
    endothelial tube lumen extension GO:0097498 ISO
    regulation of modification of postsynaptic structure GO:0099159 ISO
    skeletal muscle satellite cell migration GO:1902766 ISS
    negative regulation of reactive oxygen species biosynthetic process GO:1903427 IMP
    mitotic cleavage furrow formation GO:1903673 ISO
    mitotic cleavage furrow formation GO:1903673 ISS
    positive regulation of vascular associated smooth muscle contraction GO:1904695 IMP
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 ISO
    negative regulation of vascular associated smooth muscle cell migration GO:1904753 ISO
    positive regulation of leukocyte adhesion to vascular endothelial cell GO:1904996 IEA
    positive regulation of leukocyte adhesion to vascular endothelial cell GO:1904996 ISO
    regulation of modification of synaptic structure GO:1905244 ISO
    regulation of modification of postsynaptic actin cytoskeleton GO:1905274 IEA
    regulation of modification of postsynaptic actin cytoskeleton GO:1905274 ISO
    cellular response to chemokine GO:1990869 ISO
    cellular response to chemokine GO:1990869 ISS
    regulation of neural precursor cell proliferation GO:2000177 IMP
    positive regulation of T cell migration GO:2000406 ISO
    positive regulation of T cell migration GO:2000406 ISS
    negative regulation of motor neuron apoptotic process GO:2000672 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 HDA
    endosome GO:0005768 IEA
    endosome GO:0005768 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell cortex GO:0005938 ISO
    cell cortex GO:0005938 ISS
    cytoplasmic side of plasma membrane GO:0009898 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISS
    membrane GO:0016020 ISO
    lamellipodium GO:0030027 IDA
    axon GO:0030424 ISO
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    midbody GO:0030496 IEA
    vesicle GO:0031982 ISO
    cleavage furrow GO:0032154 IBA
    cleavage furrow GO:0032154 ISO
    cleavage furrow GO:0032154 ISS
    ruffle membrane GO:0032587 IDA
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 IBA
    dendritic spine GO:0043197 ISO
    apical junction complex GO:0043296 IEA
    apical junction complex GO:0043296 ISO
    cell periphery GO:0071944 ISO
    postsynapse GO:0098794 IEA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Rhoa in exosomes
1
Experiment ID 89
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rhoa
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLK2 1196
Affinity Capture-MS Homo sapiens
2 FGD6  
Affinity Capture-MS Homo sapiens
3 PDE12 201626
Affinity Capture-MS Homo sapiens
4 RARS2  
Affinity Capture-MS Homo sapiens
5 COG7 91949
Affinity Capture-MS Homo sapiens
6 Arhgdib 11857
Co-fractionation Mus musculus
7 Cit 12704
Affinity Capture-Western Mus musculus
8 TXNL4A 10907
Affinity Capture-MS Homo sapiens
9 GPALPP1  
Affinity Capture-MS Homo sapiens
10 Arhgap1 228359
Reconstituted Complex Mus musculus
11 SCYL1 57410
Affinity Capture-MS Homo sapiens
12 Cdkn1b  
Co-fractionation Mus musculus
13 Msn 17698
Co-fractionation Mus musculus
14 Ctnnd1 12388
Affinity Capture-Western Mus musculus
15 Ikbkg  
Affinity Capture-Western Mus musculus
16 RHOA 387
Affinity Capture-MS Homo sapiens
17 MKNK1  
Affinity Capture-MS Homo sapiens
18 POLK  
Affinity Capture-MS Homo sapiens
19 COX5A 9377
Affinity Capture-MS Homo sapiens
20 SMURF1 57154
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 Bid  
Co-fractionation Mus musculus
22 C4BPA 722
Affinity Capture-MS Homo sapiens
23 Atg16l1  
Affinity Capture-MS Mus musculus
24 Homer2  
Affinity Capture-Western Mus musculus
25 Ncl 17975
Co-fractionation Mus musculus
26 NPHS1 4868
Phenotypic Suppression Homo sapiens
27 Tgm2  
Co-fractionation Mus musculus
28 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
29 Tmem173  
Proximity Label-MS Mus musculus
30 Ezr 22350
Co-fractionation Mus musculus
31 PPL 5493
Affinity Capture-MS Homo sapiens
32 Arhgap32  
Co-fractionation Mus musculus
33 Rock2  
Co-fractionation Mus musculus
34 TRIM16 10626
Affinity Capture-MS Homo sapiens
35 Arhgdia 192662
Co-fractionation Mus musculus
36 PAPSS1 9061
Affinity Capture-MS Homo sapiens
37 FAM208B 54906
Affinity Capture-MS Homo sapiens
38 CORO7 79585
Affinity Capture-MS Homo sapiens
39 Arhgef2  
Co-fractionation Mus musculus
40 GBE1 2632
Affinity Capture-MS Homo sapiens
41 ZNF518A 9849
Affinity Capture-MS Homo sapiens
42 Ppp1r12a 17931
Co-fractionation Mus musculus
43 Fbxl19  
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
44 GPBP1  
Affinity Capture-MS Homo sapiens
45 Itpr1  
Co-fractionation Mus musculus
46 Mapt  
Affinity Capture-MS Mus musculus
47 Pitpnm1  
Co-fractionation Mus musculus
48 Rac1 19353
Co-fractionation Mus musculus
49 Ikbkb 16150
Affinity Capture-Western Mus musculus
50 Plekhg5  
FRET Mus musculus
51 Fancd2  
Affinity Capture-MS Mus musculus
52 KPRP 448834
Affinity Capture-MS Homo sapiens
53 Rasgrf1  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Rhoa is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
Axonal growth inhibition (RHOA activation) IEA Reactome
Axonal growth stimulation IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Death Receptor Signaling IEA Reactome
Deubiquitination IEA Reactome
Developmental Biology IEA Reactome
EPH-Ephrin signaling IEA Reactome
EPHA-mediated growth cone collapse IEA Reactome
EPHB-mediated forward signaling IEA Reactome
ERBB2 Regulates Cell Motility IEA Reactome
G alpha (12/13) signalling events IEA Reactome
G beta:gamma signalling through PI3Kgamma IEA Reactome
G-protein beta:gamma signalling IEA Reactome
GPCR downstream signalling IEA Reactome
GPVI-mediated activation cascade IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Metabolism of proteins IEA Reactome
Nervous system development IEA Reactome
Neutrophil degranulation IEA Reactome
Ovarian tumor domain proteases IEA Reactome
p75 NTR receptor-mediated signalling IEA Reactome
p75NTR regulates axonogenesis IEA Reactome
PCP/CE pathway IEA Reactome
PI3K/AKT activation IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Post-translational protein modification IEA Reactome
PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases IEA Reactome
RHO GTPase cycle IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases activate CIT IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate KTN1 IEA Reactome
RHO GTPases activate PKNs IEA Reactome
RHO GTPases Activate Rhotekin and Rhophilins IEA Reactome
RHO GTPases Activate ROCKs IEA Reactome
RHOA GTPase cycle IEA Reactome
RHOC GTPase cycle IEA Reactome
Sema4D in semaphorin signaling IEA Reactome
Sema4D induced cell migration and growth-cone collapse IEA Reactome
Sema4D mediated inhibition of cell attachment and migration IEA Reactome
Semaphorin interactions IEA Reactome
Signal Transduction IEA Reactome
Signaling by ERBB2 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Non-Receptor Tyrosine Kinases IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by PTK6 IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by ROBO receptors IEA Reactome
Signaling by TGF-beta Receptor Complex IEA Reactome
Signaling by TGFB family members IEA Reactome
Signaling by VEGF IEA Reactome
Signaling by WNT IEA Reactome
SLIT2:ROBO1 increases RHOA activity IEA Reactome
TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome





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