Experiment description of studies that identified TRIM16 in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for TRIM16

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ACD   Two-hybrid Homo sapiens 2 ATG16L1 55054 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 3 TRIM16L   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 HSP90AB2P 391634 Co-fractionation Homo sapiens 5 LARP1 23367 Co-fractionation Homo sapiens 6 KIF1B 23095 Co-fractionation Homo sapiens 7 LGALS2   Affinity Capture-Western Homo sapiens 8 TOMM34 10953 Co-fractionation Homo sapiens 9 POT1   Two-hybrid Homo sapiens 10 MCM7 4176 Co-fractionation Homo sapiens 11 SLC39A4 55630 Affinity Capture-MS Homo sapiens 12 PDIA4 9601 Co-fractionation Homo sapiens 13 VIM 7431 Affinity Capture-Western Homo sapiens 14 NUP62 23636 Affinity Capture-MS Homo sapiens 15 E2F1 1869 Affinity Capture-Western Homo sapiens 16 LGALS3 3958 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 17 PBK   Co-fractionation Homo sapiens 18 CUL5 8065 Affinity Capture-Western Homo sapiens 19 TRIM7   Affinity Capture-MS Homo sapiens 20 Gnpnat1   Affinity Capture-MS Mus musculus 21 USP38 84640 Affinity Capture-MS Homo sapiens 22 PPP1CA 5499 Co-fractionation Homo sapiens 23 RRAGD 58528 Affinity Capture-Western Homo sapiens 24 CLUH 23277 Co-fractionation Homo sapiens 25 KLC1 3831 Co-fractionation Homo sapiens 26 RABGAP1 23637 Co-fractionation Homo sapiens 27 RRAGB 10325 Affinity Capture-Western Homo sapiens 28 APP 351 Reconstituted Complex Homo sapiens 29 RAD50 10111 Co-fractionation Homo sapiens 30 NXF1 10482 Affinity Capture-RNA Homo sapiens 31 ACACA 31 Positive Genetic Homo sapiens 32 MBNL1 4154 Affinity Capture-MS Homo sapiens 33 TERF1 7013 Two-hybrid Homo sapiens 34 MOV10 4343 Affinity Capture-RNA Homo sapiens 35 CCR1   Affinity Capture-MS Homo sapiens 36 Sidt2   Affinity Capture-MS Mus musculus 37 MCMBP 79892 Co-fractionation Homo sapiens 38 Rhoa 11848 Affinity Capture-MS Mus musculus 39 PINK1   Affinity Capture-MS Homo sapiens 40 DEPTOR 64798 Affinity Capture-Western Homo sapiens 41 NRBP1 29959 Co-fractionation Homo sapiens 42 KIF5B 3799 Co-fractionation Homo sapiens 43 HNRNPF 3185 Co-fractionation Homo sapiens 44 PPP3CB   Affinity Capture-Western Homo sapiens 45 PSMC5 5705 Co-fractionation Homo sapiens 46 ULK1   Biochemical Activity Homo sapiens Affinity Capture-Western Homo sapiens 47 OPTN 10133 Affinity Capture-MS Homo sapiens 48 BECN1 8678 Affinity Capture-Western Homo sapiens 49 CCDC22 28952 Affinity Capture-MS Homo sapiens 50 HSPH1 10808 Co-fractionation Homo sapiens 51 ARFGEF1 10565 Co-fractionation Homo sapiens 52 TINF2   Two-hybrid Homo sapiens 53 TFEB   Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 54 CUL4A 8451 Affinity Capture-Western Homo sapiens 55 UCHL5 51377 Co-fractionation Homo sapiens View the network

Pathways in which TRIM16 is involved

No pathways found