Experiment description of studies that identified MCMBP in sEVs

1 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

Protein-protein interactions for MCMBP

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Affinity Capture-MS Homo sapiens 2 RIT1 6016 Negative Genetic Homo sapiens 3 MCM7 4176 Two-hybrid Homo sapiens Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens Co-fractionation Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 4 MCM8 84515 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 5 STAT5B 6777 Co-fractionation Homo sapiens 6 NPEPL1 79716 Co-fractionation Homo sapiens 7 MCM9 254394 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 8 PFKP 5214 Co-fractionation Homo sapiens 9 MCM3 4172 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 10 TMEM184A   Affinity Capture-MS Homo sapiens 11 PTPN1 5770 Co-fractionation Homo sapiens 12 RABGGTB 5876 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 DNAJB6 10049 Affinity Capture-MS Homo sapiens 14 CAPZB 832 Co-fractionation Homo sapiens 15 CAPN12   Affinity Capture-MS Homo sapiens 16 TECPR1 25851 Affinity Capture-MS Homo sapiens 17 MCM2 4171 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 18 DEFA5   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 KIF5B 3799 Co-fractionation Homo sapiens 20 TRIOBP 11078 Affinity Capture-MS Homo sapiens 21 SPANXN4   Affinity Capture-MS Homo sapiens 22 IMPDH2 3615 Co-fractionation Homo sapiens 23 SUV39H1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 DUSP5   Affinity Capture-MS Homo sapiens 25 MCM6 4175 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 26 PLCG1 5335 Co-fractionation Homo sapiens 27 GSPT1 2935 Co-fractionation Homo sapiens 28 PLD2 5338 Affinity Capture-MS Homo sapiens 29 DNAJC7 7266 Proximity Label-MS Homo sapiens 30 Mcm3 17215 Affinity Capture-MS Mus musculus 31 NPAS1   Affinity Capture-MS Homo sapiens 32 USP7 7874 Affinity Capture-MS Homo sapiens Co-crystal Structure Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Co-fractionation Homo sapiens 33 COMMD4 54939 Co-fractionation Homo sapiens 34 UNK   Affinity Capture-RNA Homo sapiens 35 CDK16 5127 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 PARK2   Affinity Capture-MS Homo sapiens 37 TCEAL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 TEAD3   Affinity Capture-MS Homo sapiens 39 FAM19A3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 MCM10 55388 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 YAP1 10413 Affinity Capture-MS Homo sapiens 42 TRIM16 10626 Co-fractionation Homo sapiens 43 NTRK1 4914 Affinity Capture-MS Homo sapiens 44 XPOT 11260 Co-fractionation Homo sapiens 45 RPA4   Proximity Label-MS Homo sapiens 46 APBA1   Affinity Capture-MS Homo sapiens 47 DNAJB5   Proximity Label-MS Homo sapiens 48 ZNF474   Two-hybrid Homo sapiens 49 ATP6V1D 51382 Co-fractionation Homo sapiens 50 PHLPP1   Affinity Capture-MS Homo sapiens 51 C1QTNF2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 RNF40 9810 Co-fractionation Homo sapiens 53 IPO5 3843 Co-fractionation Homo sapiens 54 CUL3 8452 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 55 DEFA1 1667 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 56 TIPRL 261726 Co-fractionation Homo sapiens 57 EGLN3   Affinity Capture-MS Homo sapiens 58 DCAF12 25853 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 59 PRKAR1A 5573 Co-fractionation Homo sapiens 60 DDX60 55601 Affinity Capture-MS Homo sapiens 61 COMMD2 51122 Co-fractionation Homo sapiens 62 WDR55   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 63 RPRD1A   Co-fractionation Homo sapiens 64 MCM4 4173 Reconstituted Complex Homo sapiens Co-fractionation Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 65 DHX30 22907 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 66 LARS 51520 Co-fractionation Homo sapiens 67 NXF1 10482 Affinity Capture-RNA Homo sapiens 68 FKBP5 2289 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 69 RPA3 6119 Proximity Label-MS Homo sapiens 70 ATP6V1A 523 Co-fractionation Homo sapiens 71 ING5   Affinity Capture-MS Homo sapiens 72 WFDC11   Affinity Capture-MS Homo sapiens 73 RHPN1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 74 ZRANB1 54764 Affinity Capture-MS Homo sapiens 75 DHX15 1665 Co-fractionation Homo sapiens 76 MRPL42   Affinity Capture-MS Homo sapiens 77 PRMT3 10196 Co-fractionation Homo sapiens 78 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 79 MCM5 4174 Co-fractionation Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 80 KEAP1 9817 Affinity Capture-MS Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 81 PRTFDC1 56952 Two-hybrid Homo sapiens 82 MAGIX 79917 Affinity Capture-MS Homo sapiens 83 TLR9   Affinity Capture-MS Homo sapiens 84 DDIT3 1649 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 85 MOV10 4343 Affinity Capture-RNA Homo sapiens 86 EIF4B 1975 Co-fractionation Homo sapiens 87 TRIP4 9325 Affinity Capture-MS Homo sapiens 88 SERBP1 26135 Affinity Capture-MS Homo sapiens 89 SNW1 22938 Two-hybrid Homo sapiens 90 KRAS 3845 Negative Genetic Homo sapiens 91 C9orf72   Affinity Capture-MS Homo sapiens 92 ATXN3 4287 Affinity Capture-MS Homo sapiens 93 C16orf71   Affinity Capture-MS Homo sapiens 94 ASL 435 Two-hybrid Homo sapiens 95 EP300 2033 Affinity Capture-MS Homo sapiens 96 PARN   Co-fractionation Homo sapiens View the network

Pathways in which MCMBP is involved

No pathways found