Gene description for Ptpn11
Gene name protein tyrosine phosphatase, non-receptor type 11
Gene symbol Ptpn11
Other names/aliases 2700084A17Rik
AW536184
PTP1D
PTP2C
SAP-2
SH-PTP2
SH-PTP3
SHP-2
Shp2
Syp
Species Mus musculus
 Database cross references - Ptpn11
ExoCarta ExoCarta_19247
Vesiclepedia VP_19247
Entrez Gene 19247
UniProt P35235  
 Ptpn11 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ptpn11
Molecular Function
    phosphotyrosine residue binding GO:0001784 IEA
    phosphotyrosine residue binding GO:0001784 ISO
    phosphoprotein phosphatase activity GO:0004721 IMP
    phosphoprotein phosphatase activity GO:0004721 ISO
    protein tyrosine phosphatase activity GO:0004725 IDA
    protein tyrosine phosphatase activity GO:0004725 ISO
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IBA
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IDA
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IMP
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 ISO
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 ISS
    insulin receptor binding GO:0005158 IEA
    insulin receptor binding GO:0005158 ISO
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    protein domain specific binding GO:0019904 ISO
    signaling receptor complex adaptor activity GO:0030159 IEA
    signaling receptor complex adaptor activity GO:0030159 ISO
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    receptor tyrosine kinase binding GO:0030971 IBA
    receptor tyrosine kinase binding GO:0030971 IPI
    receptor tyrosine kinase binding GO:0030971 ISO
    D1 dopamine receptor binding GO:0031748 ISO
    phospholipase binding GO:0043274 ISO
    insulin receptor substrate binding GO:0043560 ISO
    cadherin binding GO:0045296 IEA
    cadherin binding GO:0045296 ISO
    cell adhesion molecule binding GO:0050839 IBA
    cell adhesion molecule binding GO:0050839 IPI
    cell adhesion molecule binding GO:0050839 ISO
    peptide hormone receptor binding GO:0051428 IPI
    molecular adaptor activity GO:0060090 ISO
    histone H2AXY142 phosphatase activity GO:0140793 IEA
    protein tyrosine kinase binding GO:1990782 ISO
Biological Process
    DNA damage checkpoint signaling GO:0000077 IMP
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 ISO
    lipid metabolic process GO:0006629 IMP
    triglyceride metabolic process GO:0006641 IMP
    signal transduction GO:0007165 IMP
    epidermal growth factor receptor signaling pathway GO:0007173 IGI
    epidermal growth factor receptor signaling pathway GO:0007173 IGI
    integrin-mediated signaling pathway GO:0007229 IMP
    axonogenesis GO:0007409 IMP
    brain development GO:0007420 ISO
    heart development GO:0007507 ISO
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 ISO
    hormone-mediated signaling pathway GO:0009755 IDA
    positive regulation of signal transduction GO:0009967 IMP
    cerebellar cortex formation GO:0021697 IGI
    cerebellar cortex formation GO:0021697 IMP
    platelet formation GO:0030220 IGI
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 IMP
    negative regulation of chondrocyte differentiation GO:0032331 IMP
    negative regulation of type I interferon production GO:0032480 IEA
    negative regulation of type I interferon production GO:0032480 ISO
    microvillus organization GO:0032528 IMP
    positive regulation of interferon-beta production GO:0032728 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IGI
    regulation of cell adhesion mediated by integrin GO:0033628 ISO
    regulation of cell adhesion mediated by integrin GO:0033628 ISS
    negative regulation of cell adhesion mediated by integrin GO:0033629 IMP
    multicellular organism growth GO:0035264 IMP
    organ growth GO:0035265 IMP
    peptidyl-tyrosine dephosphorylation GO:0035335 ISO
    peptidyl-tyrosine dephosphorylation GO:0035335 ISS
    megakaryocyte development GO:0035855 IMP
    atrioventricular canal development GO:0036302 IEA
    atrioventricular canal development GO:0036302 ISO
    ERBB signaling pathway GO:0038127 IBA
    ERBB signaling pathway GO:0038127 ISO
    hormone metabolic process GO:0042445 IMP
    glucose homeostasis GO:0042593 IMP
    regulation of protein-containing complex assembly GO:0043254 IEA
    regulation of protein-containing complex assembly GO:0043254 ISO
    regulation of MAPK cascade GO:0043408 IMP
    positive regulation of ossification GO:0045778 IMP
    positive regulation of mitotic cell cycle GO:0045931 IGI
    positive regulation of D-glucose import GO:0046326 IEA
    positive regulation of D-glucose import GO:0046326 ISO
    positive regulation of insulin receptor signaling pathway GO:0046628 IEA
    positive regulation of insulin receptor signaling pathway GO:0046628 ISO
    negative regulation of insulin secretion GO:0046676 IMP
    regulation of protein export from nucleus GO:0046825 IMP
    positive regulation of hormone secretion GO:0046887 IMP
    negative regulation of hormone secretion GO:0046888 IMP
    platelet-derived growth factor receptor signaling pathway GO:0048008 IGI
    neurotrophin TRK receptor signaling pathway GO:0048011 IMP
    ephrin receptor signaling pathway GO:0048013 ISO
    ephrin receptor signaling pathway GO:0048013 ISS
    multicellular organismal reproductive process GO:0048609 IMP
    genitalia development GO:0048806 IEA
    genitalia development GO:0048806 ISO
    inner ear development GO:0048839 IEA
    inner ear development GO:0048839 ISO
    homeostasis of number of cells within a tissue GO:0048873 IGI
    homeostasis of number of cells within a tissue GO:0048873 IMP
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    negative regulation of cortisol secretion GO:0051463 IMP
    positive regulation of focal adhesion assembly GO:0051894 ISO
    Bergmann glial cell differentiation GO:0060020 IGI
    Bergmann glial cell differentiation GO:0060020 IMP
    negative regulation of growth hormone secretion GO:0060125 IMP
    face morphogenesis GO:0060325 IEA
    face morphogenesis GO:0060325 ISO
    intestinal epithelial cell migration GO:0061582 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IBA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IGI
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISS
    cellular response to mechanical stimulus GO:0071260 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 ISS
Subcellular Localization
    stress fiber GO:0001725 ISS
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 ISS
    cell junction GO:0030054 ISS
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    plasma membrane raft GO:0044853 ISO
 Experiment description of studies that identified Ptpn11 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ptpn11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gab2  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
2 Fasn 14104
Co-fractionation Mus musculus
Affinity Capture-MS Mus musculus
3 Ghr 14600
Affinity Capture-Western Mus musculus
4 SPRY2 10253
PCA Homo sapiens
5 Il6st 16195
Affinity Capture-Western Mus musculus
6 Ptpra 19262
Co-fractionation Mus musculus
7 Btla  
Affinity Capture-Western Mus musculus
8 Gab1  
Affinity Capture-Western Mus musculus
9 Shc1  
Affinity Capture-Western Mus musculus
10 Itch 16396
Affinity Capture-Western Mus musculus
11 Frs2  
Affinity Capture-Western Mus musculus
12 Inpp5d 16331
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
13 Grin2b  
Affinity Capture-Western Mus musculus
14 Itsn1 16443
Affinity Capture-Western Mus musculus
PCA Mus musculus
15 Src 20779
Co-fractionation Mus musculus
16 Lepr  
Affinity Capture-Western Mus musculus
17 FRS2 10818
Affinity Capture-Western Homo sapiens
18 Rfwd2 26374
Affinity Capture-Western Mus musculus
19 Abl1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
20 Tek  
Reconstituted Complex Mus musculus
21 Grin1  
Affinity Capture-Western Mus musculus
22 Cblb 208650
Affinity Capture-Western Mus musculus
23 Sirpa  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
24 Egfr 13649
Affinity Capture-Western Mus musculus
25 Sos1  
Co-fractionation Mus musculus
26 Cbl 12402
Affinity Capture-Western Mus musculus
27 Tnfrsf14  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Ptpn11 is involved
PathwayEvidenceSource
Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) IEA Reactome
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Costimulation by the CD28 family IEA Reactome
CTLA4 inhibitory signaling IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology IEA Reactome
Downstream signal transduction IEA Reactome
Downstream signaling of activated FGFR1 IEA Reactome
Downstream signaling of activated FGFR2 IEA Reactome
Downstream signaling of activated FGFR3 IEA Reactome
Downstream signaling of activated FGFR4 IEA Reactome
FRS-mediated FGFR1 signaling IEA Reactome
FRS-mediated FGFR2 signaling IEA Reactome
FRS-mediated FGFR3 signaling IEA Reactome
FRS-mediated FGFR4 signaling IEA Reactome
GAB1 signalosome IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GPVI-mediated activation cascade IEA Reactome
Hemostasis IEA Reactome
IGF1R signaling cascade IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Insulin receptor signalling cascade IEA Reactome
Interferon alpha/beta signaling IEA Reactome
Interferon Signaling IEA Reactome
Interleukin-20 family signaling IEA Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling IEA Reactome
Interleukin-6 family signaling IEA Reactome
Interleukin-6 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
IRS-mediated signalling IEA Reactome
IRS-related events triggered by IGF1R IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1 (ERK2) activation IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MAPK3 (ERK1) activation IEA Reactome
MET activates PTPN11 IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
Nervous system development IEA Reactome
PD-1 signaling IEA Reactome
PECAM1 interactions IEA Reactome
PI-3K cascade:FGFR1 IEA Reactome
PI-3K cascade:FGFR2 IEA Reactome
PI-3K cascade:FGFR3 IEA Reactome
PI-3K cascade:FGFR4 IEA Reactome
PI3K Cascade IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet homeostasis IEA Reactome
Platelet sensitization by LDL IEA Reactome
RAF-independent MAPK1/3 activation IEA Reactome
Regulation of IFNA/IFNB signaling IEA Reactome
Regulation of RUNX1 Expression and Activity IEA Reactome
RET signaling IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Signal Transduction IEA Reactome
Signaling by CSF3 (G-CSF) IEA Reactome
Signaling by EGFR IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by Insulin receptor IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by MET IEA Reactome
Signaling by PDGF IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by SCF-KIT IEA Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Tie2 Signaling IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
Transcriptional regulation by RUNX1 IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





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