Gene description for Pdgfrb
Gene name platelet derived growth factor receptor, beta polypeptide
Gene symbol Pdgfrb
Other names/aliases AI528809
CD140b
PDGFR-1
Pdgfr
Species Mus musculus
 Database cross references - Pdgfrb
ExoCarta ExoCarta_18596
Vesiclepedia VP_18596
Entrez Gene 18596
UniProt P05622  
 Pdgfrb identified in sEVs derived from the following tissue/cell type
Breast cancer cells 34112803    
Breast cancer cells 34112803    
Colon cancer cells 37309723    
Fibroblasts 23260141    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pdgfrb
Molecular Function
    protein kinase activity GO:0004672 ISO
    protein tyrosine kinase activity GO:0004713 ISO
    protein tyrosine kinase activity GO:0004713 ISS
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IEA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor receptor activity GO:0005017 IDA
    platelet-derived growth factor receptor activity GO:0005017 ISO
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IBA
    platelet-derived growth factor beta-receptor activity GO:0005019 ISO
    platelet-derived growth factor beta-receptor activity GO:0005019 ISS
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    signaling receptor binding GO:0005102 IPI
    signaling receptor binding GO:0005102 ISO
    platelet-derived growth factor receptor binding GO:0005161 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    boss receptor activity GO:0008288 IEA
    kinase activity GO:0016301 IMP
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 ISO
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    vascular endothelial growth factor binding GO:0038085 ISO
    phosphatidylinositol 3-kinase binding GO:0043548 ISO
    platelet-derived growth factor binding GO:0048407 IBA
    platelet-derived growth factor binding GO:0048407 ISO
    platelet-derived growth factor binding GO:0048407 TAS
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    angiogenesis GO:0001525 IBA
    blood vessel development GO:0001568 IEP
    in utero embryonic development GO:0001701 IMP
    kidney development GO:0001822 IMP
    tissue homeostasis GO:0001894 IMP
    glycosaminoglycan biosynthetic process GO:0006024 ISO
    chromatin remodeling GO:0006338 IEA
    signal transduction GO:0007165 IDA
    signal transduction GO:0007165 ISO
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    multicellular organism development GO:0007275 IBA
    excretion GO:0007588 IMP
    positive regulation of cell population proliferation GO:0008284 ISO
    insulin receptor signaling pathway GO:0008286 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    positive regulation of smooth muscle cell migration GO:0014911 IBA
    positive regulation of smooth muscle cell migration GO:0014911 IGI
    positive regulation of smooth muscle cell migration GO:0014911 IMP
    positive regulation of smooth muscle cell migration GO:0014911 ISO
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 ISO
    peptidyl-tyrosine phosphorylation GO:0018108 ISS
    hemopoiesis GO:0030097 IMP
    adrenal gland development GO:0030325 IGI
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of cell migration GO:0030335 ISS
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    regulation of actin cytoskeleton organization GO:0032956 IGI
    positive regulation of collagen biosynthetic process GO:0032967 ISO
    positive regulation of Rho protein signal transduction GO:0035025 ISO
    cell migration involved in vasculogenesis GO:0035441 IMP
    intracellular signal transduction GO:0035556 ISO
    metanephric mesenchymal cell migration GO:0035789 ISO
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 ISO
    positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway GO:0035793 IMP
    aorta morphogenesis GO:0035909 IGI
    cellular response to platelet-derived growth factor stimulus GO:0036120 IEA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway GO:0038091 ISO
    positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway GO:0038091 ISS
    Kit signaling pathway GO:0038109 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of MAP kinase activity GO:0043406 IDA
    positive regulation of mitotic nuclear division GO:0045840 IDA
    phosphatidylinositol metabolic process GO:0046488 ISO
    protein autophosphorylation GO:0046777 IDA
    protein autophosphorylation GO:0046777 ISO
    protein autophosphorylation GO:0046777 ISS
    platelet-derived growth factor receptor signaling pathway GO:0048008 IMP
    platelet-derived growth factor receptor signaling pathway GO:0048008 IPI
    platelet-derived growth factor receptor signaling pathway GO:0048008 ISO
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IEA
    positive regulation of fibroblast proliferation GO:0048146 ISO
    embryonic organ development GO:0048568 IEP
    positive regulation of smooth muscle cell proliferation GO:0048661 IMP
    positive regulation of smooth muscle cell proliferation GO:0048661 ISO
    skeletal system morphogenesis GO:0048705 IMP
    smooth muscle tissue development GO:0048745 IMP
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 IMP
    positive regulation of calcium-mediated signaling GO:0050850 ISO
    positive regulation of chemotaxis GO:0050921 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISS
    cardiac myofibril assembly GO:0055003 IGI
    cell chemotaxis GO:0060326 IBA
    cell chemotaxis GO:0060326 ISO
    cell chemotaxis GO:0060326 ISS
    lung growth GO:0060437 ISO
    cardiac vascular smooth muscle cell differentiation GO:0060947 TAS
    cell migration involved in coronary angiogenesis GO:0060981 IMP
    retina vasculature development in camera-type eye GO:0061298 IGI
    retina vasculature development in camera-type eye GO:0061298 IMP
    cellular response to hydrogen peroxide GO:0070301 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    smooth muscle cell chemotaxis GO:0071670 IGI
    metanephric glomerular mesangium development GO:0072223 IEP
    metanephric glomerular mesangial cell proliferation involved in metanephros development GO:0072262 IMP
    metanephric glomerular capillary formation GO:0072277 IGI
    positive regulation of calcium ion import GO:0090280 IMP
    ruffle assembly GO:0097178 IDA
    response to ceramide GO:0106096 IMP
    positive regulation of reactive oxygen species metabolic process GO:2000379 IMP
    positive regulation of hepatic stellate cell activation GO:2000491 ISO
    positive regulation of DNA biosynthetic process GO:2000573 IMP
Subcellular Localization
    ruffle GO:0001726 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    membrane GO:0016020 ISO
    apical plasma membrane GO:0016324 ISO
    apical plasma membrane GO:0016324 ISS
    cytoplasmic vesicle GO:0031410 IEA
    lysosomal lumen GO:0043202 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    receptor complex GO:0043235 IBA
 Experiment description of studies that identified Pdgfrb in sEVs
1
Experiment ID 520
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd9
Enriched markers
✔
Golga2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34112803    
Organism Mus musculus
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name EO771
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 521
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd9
Enriched markers
✔
Golga2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34112803    
Organism Mus musculus
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name PyMT
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pdgfrb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCK2 8440
Reconstituted Complex Homo sapiens
2 Twist1  
Proximity Label-MS Mus musculus
3 CBL 867
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 Grb2 14784
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
5 Plcg1  
Reconstituted Complex Mus musculus
6 Cbl 12402
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
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