Gene description for Laptm5
Gene name lysosomal-associated protein transmembrane 5
Gene symbol Laptm5
Other names/aliases E3
Species Mus musculus
 Database cross references - Laptm5
ExoCarta ExoCarta_16792
Vesiclepedia VP_16792
Entrez Gene 16792
UniProt Q61168  
 Laptm5 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Laptm5
Molecular Function
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    ubiquitin protein ligase binding GO:0031625 ISO
    protein sequestering activity GO:0140311 IMP
Biological Process
    defense response to tumor cell GO:0002357 ISO
    positive regulation of cytokine production involved in immune response GO:0002720 IMP
    protein targeting to lysosome GO:0006622 IDA
    intracellular protein transport GO:0006886 IMP
    induction of programmed cell death GO:0012502 ISO
    positive regulation of protein ubiquitination GO:0031398 IMP
    negative regulation of type II interferon production GO:0032689 IMP
    negative regulation of interleukin-2 production GO:0032703 IMP
    positive regulation of interleukin-12 production GO:0032735 IMP
    positive regulation of interleukin-6 production GO:0032755 IMP
    positive regulation of MAPK cascade GO:0043410 IMP
    negative regulation of activated T cell proliferation GO:0046007 IMP
    negative regulation of T cell receptor signaling pathway GO:0050860 IMP
    negative regulation of T cell activation GO:0050868 IMP
    negative regulation of B cell activation GO:0050869 IMP
    positive regulation of macrophage cytokine production GO:0060907 IMP
    protein localization to lysosome GO:0061462 IMP
    Golgi to lysosome transport GO:0090160 IDA
    positive regulation of lysosomal membrane permeability GO:0097214 ISO
    negative regulation of pre-B cell receptor expression GO:0140646 IMP
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IMP
    positive regulation of proteolysis involved in protein catabolic process GO:1903052 IMP
    positive regulation of tumor necrosis factor-mediated signaling pathway GO:1903265 IMP
    negative regulation of autophagic cell death GO:1904093 ISO
    cellular response to leukemia inhibitory factor GO:1990830 IEP
    positive regulation of ubiquitin-dependent protein catabolic process GO:2000060 IDA
    positive regulation of ubiquitin-dependent protein catabolic process GO:2000060 IMP
    positive regulation of receptor catabolic process GO:2000646 IMP
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 ISO
    transport vesicle GO:0030133 ISO
    cytoplasmic vesicle GO:0031410 ISO
    protein-containing complex GO:0032991 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Laptm5 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Laptm5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ncf4  
Co-fractionation Mus musculus
2 Nedd4 17999
Reconstituted Complex Mus musculus
3 Itgb2 16414
Co-fractionation Mus musculus
4 Usp7  
Affinity Capture-MS Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Laptm5 is involved
No pathways found





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