Gene description for Adrbk1
Gene name adrenergic receptor kinase, beta 1
Gene symbol Adrbk1
Other names/aliases Adrbk-1
Bark-1
GRK2
beta ARK
betaARK1
Species Mus musculus
 Database cross references - Adrbk1
ExoCarta ExoCarta_110355
Vesiclepedia VP_110355
Entrez Gene 110355
UniProt Q99MK8  
 Adrbk1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Adrbk1
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    G protein-coupled receptor binding GO:0001664 ISO
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IDA
    G protein-coupled receptor kinase activity GO:0004703 IBA
    G protein-coupled receptor kinase activity GO:0004703 IDA
    G protein-coupled receptor kinase activity GO:0004703 IMP
    G protein-coupled receptor kinase activity GO:0004703 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    Edg-2 lysophosphatidic acid receptor binding GO:0031755 IEA
    Edg-2 lysophosphatidic acid receptor binding GO:0031755 ISO
    delta-type opioid receptor binding GO:0031850 ISO
    kappa-type opioid receptor binding GO:0031851 ISO
    beta-adrenergic receptor kinase activity GO:0047696 IEA
    beta-adrenergic receptor kinase activity GO:0047696 ISO
    scaffold protein binding GO:0097110 ISO
Biological Process
    regulation of the force of heart contraction GO:0002026 IBA
    regulation of the force of heart contraction GO:0002026 IGI
    desensitization of G protein-coupled receptor signaling pathway GO:0002029 IBA
    desensitization of G protein-coupled receptor signaling pathway GO:0002029 IGI
    desensitization of G protein-coupled receptor signaling pathway GO:0002029 IMP
    desensitization of G protein-coupled receptor signaling pathway GO:0002029 ISO
    negative regulation of the force of heart contraction by chemical signal GO:0003108 IEA
    negative regulation of the force of heart contraction by chemical signal GO:0003108 ISO
    protein phosphorylation GO:0006468 IDA
    response to oxidative stress GO:0006979 ISO
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    tachykinin receptor signaling pathway GO:0007217 IEA
    tachykinin receptor signaling pathway GO:0007217 ISO
    heart development GO:0007507 IMP
    positive regulation of muscle cell apoptotic process GO:0010661 ISO
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 ISO
    response to organic cyclic compound GO:0014070 ISO
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-threonine phosphorylation GO:0018107 IDA
    viral genome replication GO:0019079 IEA
    viral genome replication GO:0019079 ISO
    receptor internalization GO:0031623 IEA
    receptor internalization GO:0031623 ISO
    positive regulation of interleukin-6 production GO:0032755 ISO
    negative regulation of peptidyl-serine phosphorylation GO:0033137 ISO
    positive regulation of catecholamine secretion GO:0033605 ISO
    vasodilation GO:0042311 ISO
    follicle-stimulating hormone signaling pathway GO:0042699 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of G protein-coupled receptor signaling pathway GO:0045744 ISO
    positive regulation of vasoconstriction GO:0045907 ISO
    negative regulation of striated muscle contraction GO:0045988 IEA
    negative regulation of striated muscle contraction GO:0045988 ISO
    negative regulation of D-glucose import GO:0046325 ISO
    symbiont entry into host cell GO:0046718 IEA
    symbiont entry into host cell GO:0046718 ISO
    positive regulation of fibroblast proliferation GO:0048146 ISO
    oligodendrocyte differentiation GO:0048709 ISO
    maintenance of protein location in nucleus GO:0051457 ISO
    cardiac muscle contraction GO:0060048 IEA
    cardiac muscle contraction GO:0060048 ISO
    cellular response to glucose stimulus GO:0071333 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
    negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0106072 ISO
    negative regulation of cellular response to insulin stimulus GO:1900077 ISO
    negative regulation of relaxation of smooth muscle GO:1901081 IMP
    negative regulation of relaxation of smooth muscle GO:1901081 ISO
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 ISO
    cellular response to chemokine GO:1990869 ISO
    positive regulation of excitatory postsynaptic potential GO:2000463 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IEA
    caveola GO:0005901 ISO
    cilium GO:0005929 TAS
    postsynaptic density GO:0014069 ISO
    membrane GO:0016020 IDA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    axon GO:0030424 ISO
    cytoplasmic side of mitochondrial outer membrane GO:0032473 IEA
    dendritic spine GO:0043197 ISO
    dendritic shaft GO:0043198 ISO
    presynapse GO:0098793 IEA
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Adrbk1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Adrbk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mdm2  
Affinity Capture-Western Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Scnn1b  
Biochemical Activity Mus musculus
4 Nedd4 17999
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 MTNR1A  
Reconstituted Complex Homo sapiens
7 Pdcl  
Co-fractionation Mus musculus
8 Adra2a  
Affinity Capture-Western Mus musculus
9 Nedd4l 83814
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
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