Gene description for UBE2L3
Gene name ubiquitin-conjugating enzyme E2L 3
Gene symbol UBE2L3
Other names/aliases E2-F1
L-UBC
UBCH7
UbcM4
Species Homo sapiens
 Database cross references - UBE2L3
ExoCarta ExoCarta_7332
Vesiclepedia VP_7332
Entrez Gene 7332
HGNC 12488
MIM 603721
UniProt P68036  
 UBE2L3 identified in sEVs derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
 Gene ontology annotations for UBE2L3
Molecular Function
    transcription coactivator activity GO:0003713 IDA
    RNA binding GO:0003723 HDA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin-protein transferase activity GO:0004842 IMP
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    enzyme binding GO:0019899 TAS
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin conjugating enzyme activity GO:0061631 IBA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
    ubiquitin-protein transferase activator activity GO:0097027 IGI
Biological Process
    protein polyubiquitination GO:0000209 IDA
    regulation of DNA-templated transcription GO:0006355 IDA
    regulation of DNA-templated transcription GO:0006355 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    cell population proliferation GO:0008283 IMP
    protein ubiquitination GO:0016567 IDA
    positive regulation of protein ubiquitination GO:0031398 IGI
    protein modification process GO:0036211 TAS
    cell cycle phase transition GO:0044770 IMP
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of ubiquitin-protein transferase activity GO:0051443 IGI
    protein K11-linked ubiquitination GO:0070979 IBA
    protein K11-linked ubiquitination GO:0070979 IDA
    cellular response to steroid hormone stimulus GO:0071383 IMP
    cellular response to glucocorticoid stimulus GO:0071385 IDA
    positive regulation of protein targeting to mitochondrion GO:1903955 HMP
Subcellular Localization
    ubiquitin ligase complex GO:0000151 TAS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified UBE2L3 in sEVs
1
Experiment ID 412
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
2
Experiment ID 414
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
3
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
8
Experiment ID 407
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
9
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 405
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
14
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 417
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 411
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 254
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 255
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 256
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
23
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 413
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 406
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 415
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
29
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 408
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
31
Experiment ID 409
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 416
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 275
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
34
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
35
Experiment ID 410
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for UBE2L3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 LIG4 3981
Two-hybrid Homo sapiens
3 HSPE1 3336
Co-fractionation Homo sapiens
4 SNX3 8724
Co-fractionation Homo sapiens
5 XIAP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 HEBP1 50865
Co-fractionation Homo sapiens
7 UBE2A  
Co-fractionation Homo sapiens
8 WWP1 11059
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 SRPRB 58477
Co-fractionation Homo sapiens
10 DUSP8  
Affinity Capture-MS Homo sapiens
11 NDUFA7 4701
Co-fractionation Homo sapiens
12 UBA1 7317
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 BRCA1 672
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
14 RTFDC1  
Reconstituted Complex Homo sapiens
15 UBE3A 7337
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 USP9X 8239
Co-fractionation Homo sapiens
17 UBC 7316
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
18 TRIM55  
Reconstituted Complex Homo sapiens
19 CCNB1IP1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
20 MID1 4281
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 PIR 8544
Co-fractionation Homo sapiens
22 LTN1 26046
Reconstituted Complex Homo sapiens
23 RNF216  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 EIF4E2  
Affinity Capture-Western Homo sapiens
25 OTUD3 23252
Biochemical Activity Homo sapiens
26 MAP1LC3B 81631
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
27 IRF2BP1 26145
Reconstituted Complex Homo sapiens
28 PPP1R12A 4659
Cross-Linking-MS (XL-MS) Homo sapiens
29 PJA1  
Reconstituted Complex Homo sapiens
30 TRIM28 10155
Reconstituted Complex Homo sapiens
31 TBCB 1155
Cross-Linking-MS (XL-MS) Homo sapiens
32 MCM2 4171
Affinity Capture-MS Homo sapiens
33 PTRHD1 391356
Co-fractionation Homo sapiens
34 DAZAP1 26528
Co-fractionation Homo sapiens
35 RNF103  
Two-hybrid Homo sapiens
36 HERC4 26091
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
37 ANKRD36 375248
Cross-Linking-MS (XL-MS) Homo sapiens
38 HERC2 8924
Reconstituted Complex Homo sapiens
39 BIRC3 330
Affinity Capture-Western Homo sapiens
40 ZNF598 90850
Affinity Capture-MS Homo sapiens
41 ARIH2 10425
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
42 NEDD4 4734
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
43 SOD1 6647
Co-fractionation Homo sapiens
44 TP53BP1 7158
Affinity Capture-Western Homo sapiens
45 RNF11 26994
Affinity Capture-MS Homo sapiens
46 ANKIB1 54467
Reconstituted Complex Homo sapiens
47 HSPA8 3312
Cross-Linking-MS (XL-MS) Homo sapiens
48 NCOA1  
Affinity Capture-Western Homo sapiens
49 MDH2 4191
Co-fractionation Homo sapiens
50 AURKA 6790
Affinity Capture-MS Homo sapiens
51 ACAT2 39
Co-fractionation Homo sapiens
52 RNF144A  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 UBE4B 10277
Co-fractionation Homo sapiens
54 RNF113B  
Two-hybrid Homo sapiens
55 PCK2 5106
Co-fractionation Homo sapiens
56 RBX1 9978
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
57 USP26  
Co-fractionation Homo sapiens
58 PARK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
59 SKP2  
Affinity Capture-Western Homo sapiens
60 MEX3C 51320
Reconstituted Complex Homo sapiens
61 CBL 867
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
62 YAP1 10413
Co-fractionation Homo sapiens
63 RNF213 57674
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
64 MAPK6  
Two-hybrid Homo sapiens
65 UBR5 51366
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
66 MKRN2 23609
Reconstituted Complex Homo sapiens
67 HERC6  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
68 UBB 7314
Affinity Capture-MS Homo sapiens
69 PSMD2 5708
Co-fractionation Homo sapiens
70 TRIM21 6737
Reconstituted Complex Homo sapiens
71 ATXN3 4287
Reconstituted Complex Homo sapiens
72 PIAS2  
Reconstituted Complex Homo sapiens
73 HACE1 57531
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
74 HECTD2  
Reconstituted Complex Homo sapiens
75 UBXN1 51035
Co-fractionation Homo sapiens
76 RNF125  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
77 RAB10 10890
Co-fractionation Homo sapiens
78 BRAP  
Reconstituted Complex Homo sapiens
79 ITCH 83737
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
80 VDAC2 7417
Co-fractionation Homo sapiens
81 DNM1L 10059
Affinity Capture-MS Homo sapiens
82 VPS11 55823
Reconstituted Complex Homo sapiens
83 TRIM23 373
Reconstituted Complex Homo sapiens
84 ZPR1 8882
Co-fractionation Homo sapiens
85 BAG3 9531
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
86 RNF168  
Reconstituted Complex Homo sapiens
87 BIRC2  
Affinity Capture-Western Homo sapiens
88 TSNAXIP1 55815
Co-fractionation Homo sapiens
89 MMACHC  
Co-fractionation Homo sapiens
90 RNF144B  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
91 CACNA1A  
Affinity Capture-Western Homo sapiens
92 SUGP1  
Co-fractionation Homo sapiens
93 RNF19A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
94 AHCYL1 10768
Co-fractionation Homo sapiens
95 NR3C1 2908
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
96 RNF170 81790
Reconstituted Complex Homo sapiens
97 RELA 5970
Biochemical Activity Homo sapiens
98 UBXN7 26043
Co-fractionation Homo sapiens
99 RNF14 9604
Reconstituted Complex Homo sapiens
100 UBE2L3 7332
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
101 Trp53  
Biochemical Activity Mus musculus
102 NFKBIA  
Biochemical Activity Homo sapiens
103 MEX3A  
Reconstituted Complex Homo sapiens
104 RNF150  
Reconstituted Complex Homo sapiens
105 TRAF6 7189
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
106 SF1 7536
Co-fractionation Homo sapiens
107 PTEN 5728
Affinity Capture-Western Homo sapiens
108 FBXW2  
Affinity Capture-MS Homo sapiens
109 Nedd4l 83814
Reconstituted Complex Mus musculus
110 POLR2A 5430
Reconstituted Complex Homo sapiens
111 FN1 2335
Affinity Capture-MS Homo sapiens
112 PITPNA 5306
Co-fractionation Homo sapiens
113 CBLB 868
Reconstituted Complex Homo sapiens
114 UBE2D2 7322
Co-fractionation Homo sapiens
115 CUL9  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
116 RNF220  
Reconstituted Complex Homo sapiens
117 FOS 2353
Biochemical Activity Homo sapiens
118 PIAS1 8554
Reconstituted Complex Homo sapiens
119 MYC  
Affinity Capture-Western Homo sapiens
120 HECW2  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
121 FBXO2 26232
Reconstituted Complex Homo sapiens
122 MSI2 124540
Co-fractionation Homo sapiens
123 ESD 2098
Co-fractionation Homo sapiens
124 TIGAR 57103
Co-fractionation Homo sapiens
125 SNX12 29934
Co-fractionation Homo sapiens
126 MYCBP2 23077
Reconstituted Complex Homo sapiens
127 CACNA1B 774
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
128 RNF2  
Reconstituted Complex Homo sapiens
129 Nedd4 17999
Reconstituted Complex Mus musculus
130 RNF114 55905
Reconstituted Complex Homo sapiens
131 TRIM39  
Two-hybrid Homo sapiens
132 MDM2  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
133 NEDD4L 23327
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
134 HUWE1 10075
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
135 CBLC  
Two-hybrid Homo sapiens
136 STUB1 10273
Reconstituted Complex Homo sapiens
137 HERC1 8925
Reconstituted Complex Homo sapiens
138 HECTD1 25831
Reconstituted Complex Homo sapiens
139 TRAF7  
Two-hybrid Homo sapiens
140 DTX3  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
141 TP53 7157
Biochemical Activity Homo sapiens
142 RPS27A 6233
Cross-Linking-MS (XL-MS) Homo sapiens
143 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
144 MUL1  
Two-hybrid Homo sapiens
145 TRIM13  
Reconstituted Complex Homo sapiens
146 PARP1 142
Affinity Capture-MS Homo sapiens
147 HECW1  
Reconstituted Complex Homo sapiens
148 UBA5 79876
Reconstituted Complex Homo sapiens
149 SMAD7  
Protein-peptide Homo sapiens
150 USP34 9736
Co-fractionation Homo sapiens
151 RNF19B  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
152 HECTD4 283450
Reconstituted Complex Homo sapiens
153 RNF31 55072
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
154 UBA7 7318
Biochemical Activity Homo sapiens
155 MRPL53  
Co-fractionation Homo sapiens
156 UBA52 7311
Cross-Linking-MS (XL-MS) Homo sapiens
157 SMURF1 57154
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
158 MKRN3  
Two-hybrid Homo sapiens
159 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
160 RNF20 56254
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
161 OTUB1 55611
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
162 Itch 16396
Reconstituted Complex Mus musculus
163 UBOX5  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
164 UCHL3 7347
Co-fractionation Homo sapiens
165 TRIM25 7706
Reconstituted Complex Homo sapiens
166 UBE3C 9690
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
167 TRIP12 9320
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
168 UBR4 23352
Reconstituted Complex Homo sapiens
169 WWP2 11060
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
170 PSMA5 5686
Co-fractionation Homo sapiens
171 UBE2B 7320
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
172 AREL1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
173 TRIM31  
Reconstituted Complex Homo sapiens
174 RNF182  
Two-hybrid Homo sapiens
175 RNF111 54778
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
176 HERC3 8916
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
177 DUSP4 1846
Affinity Capture-MS Homo sapiens
178 Park2  
Reconstituted Complex Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Biochemical Activity Rattus norvegicus
179 KRAS 3845
Negative Genetic Homo sapiens
180 SMURF2 64750
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
181 RC3H1 149041
Reconstituted Complex Homo sapiens
182 UBA6 55236
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
183 UBASH3A 53347
FRET Homo sapiens
184 RBCK1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
185 UBE2V2 7336
Co-fractionation Homo sapiens
186 UFM1 51569
Co-fractionation Homo sapiens
187 ARIH1 25820
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
188 Ubox5  
Two-hybrid Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here