Gene description for HERC1
Gene name HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Gene symbol HERC1
Other names/aliases p532
p619
Species Homo sapiens
 Database cross references - HERC1
ExoCarta ExoCarta_8925
Vesiclepedia VP_8925
Entrez Gene 8925
HGNC 4867
MIM 605109
UniProt Q15751  
 HERC1 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HERC1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
Biological Process
    autophagy GO:0006914 IEA
    negative regulation of autophagy GO:0010507 IEA
    protein ubiquitination GO:0016567 IEA
    cerebellar Purkinje cell differentiation GO:0021702 IEA
    corpus callosum development GO:0022038 IEA
    neuron projection development GO:0031175 IEA
    neuromuscular process controlling balance GO:0050885 IEA
Subcellular Localization
    cytoplasm GO:0005737 TAS
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IEA
    membrane GO:0016020 IEA
 Experiment description of studies that identified HERC1 in sEVs
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HERC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SENP3 26168
Affinity Capture-MS Homo sapiens
2 C2CD4A  
Affinity Capture-MS Homo sapiens
3 ZNF555  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TSC2 7249
Affinity Capture-Western Homo sapiens
5 DUXAP10  
Affinity Capture-MS Homo sapiens
6 PKM 5315
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
7 RAB15 376267
Affinity Capture-MS Homo sapiens
8 RCL1 10171
Affinity Capture-MS Homo sapiens
9 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BRCA1 672
Affinity Capture-MS Homo sapiens
11 RPL10 6134
Affinity Capture-MS Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 TIGD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 UBC 7316
Reconstituted Complex Homo sapiens
15 ADAMTS13 11093
Affinity Capture-MS Homo sapiens
16 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
17 MORC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PTPN1 5770
Proximity Label-MS Homo sapiens
19 SOX9  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
20 SNX6 58533
Affinity Capture-MS Homo sapiens
21 CCDC65  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CD247 919
Affinity Capture-MS Homo sapiens
23 C1orf87  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TRMU  
Affinity Capture-MS Homo sapiens
25 D2HGDH 728294
Affinity Capture-MS Homo sapiens
26 HES6  
Affinity Capture-MS Homo sapiens
27 USP18  
Affinity Capture-MS Homo sapiens
28 PRC1 9055
Affinity Capture-MS Homo sapiens
29 PPEF1  
Affinity Capture-MS Homo sapiens
30 YWHAH 7533
Affinity Capture-MS Homo sapiens
31 WDFY3 23001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
33 C2CD4B  
Affinity Capture-MS Homo sapiens
34 TMEM136  
Affinity Capture-MS Homo sapiens
35 FBXW5  
Affinity Capture-MS Homo sapiens
36 IQCF2  
Affinity Capture-MS Homo sapiens
37 KIF14 9928
Affinity Capture-MS Homo sapiens
38 FOXP1 27086
Protein-RNA Homo sapiens
39 UNK  
Affinity Capture-RNA Homo sapiens
40 PXMP2  
Proximity Label-MS Homo sapiens
41 RABGEF1 27342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 C3orf20  
Affinity Capture-MS Homo sapiens
43 UCN3  
Affinity Capture-MS Homo sapiens
44 SPDYC  
Affinity Capture-MS Homo sapiens
45 PSMD14 10213
Affinity Capture-MS Homo sapiens
46 CHAC2 494143
Affinity Capture-MS Homo sapiens
47 TPTE2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 DIRAS3  
Proximity Label-MS Homo sapiens
49 TTLL1  
Affinity Capture-MS Homo sapiens
50 NOS2  
Affinity Capture-MS Homo sapiens
51 ZBBX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DHFRL1  
Proximity Label-MS Homo sapiens
53 ZNF563  
Affinity Capture-MS Homo sapiens
54 UBE2D3 7323
Reconstituted Complex Homo sapiens
55 MYL12A 10627
Co-fractionation Homo sapiens
56 PEX11A  
Affinity Capture-MS Homo sapiens
57 UBXN6 80700
Affinity Capture-MS Homo sapiens
58 UPP1 7378
Affinity Capture-MS Homo sapiens
59 KLHL14 57565
Affinity Capture-MS Homo sapiens
60 ANLN 54443
Affinity Capture-MS Homo sapiens
61 TTI1 9675
Affinity Capture-MS Homo sapiens
62 UBE2S  
Reconstituted Complex Homo sapiens
63 GCNT3 9245
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 WHAMMP3  
Affinity Capture-MS Homo sapiens
65 TTC26 79989
Affinity Capture-MS Homo sapiens
66 PPM1M  
Affinity Capture-MS Homo sapiens
67 BYSL 705
Co-fractionation Homo sapiens
68 GRIPAP1 56850
Co-fractionation Homo sapiens
69 Slain2  
Affinity Capture-MS Mus musculus
70 UBE2L3 7332
Reconstituted Complex Homo sapiens
71 IRAK1BP1 134728
Affinity Capture-MS Homo sapiens
72 KCMF1 56888
Co-fractionation Homo sapiens
73 AKT3  
Affinity Capture-MS Homo sapiens
74 CUL3 8452
Affinity Capture-MS Homo sapiens
75 EGFR 1956
Affinity Capture-MS Homo sapiens
76 METTL21B  
Affinity Capture-MS Homo sapiens
77 ZNF223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 ECHS1 1892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 SSH3 54961
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 MLF1  
Affinity Capture-MS Homo sapiens
81 UBE2D2 7322
Reconstituted Complex Homo sapiens
82 SMOX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 ARHGAP29 9411
Affinity Capture-MS Homo sapiens
84 NPAS1  
Affinity Capture-MS Homo sapiens
85 Mis12  
Affinity Capture-MS Mus musculus
86 PABPC5 140886
Affinity Capture-MS Homo sapiens
87 NDC80 10403
Proximity Label-MS Homo sapiens
88 FLII 2314
Affinity Capture-MS Homo sapiens
89 CLTC 1213
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
90 ZNF136  
Affinity Capture-MS Homo sapiens
91 RSPH10B2  
Affinity Capture-MS Homo sapiens
92 NEUROG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 ZNF610  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 RAPSN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 HERC1 8925
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
96 VPS36 51028
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 C1orf177  
Affinity Capture-MS Homo sapiens
98 TFDP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 TTC6  
Affinity Capture-MS Homo sapiens
100 FOXRED2  
Affinity Capture-MS Homo sapiens
101 MCM5 4174
Affinity Capture-MS Homo sapiens
102 ATAT1  
Affinity Capture-MS Homo sapiens
103 DFFA 1676
Affinity Capture-MS Homo sapiens
104 EDEM1  
Affinity Capture-MS Homo sapiens
105 AGMAT  
Affinity Capture-MS Homo sapiens
106 ABL1 25
Biochemical Activity Homo sapiens
107 ZNF571 51276
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
109 FAM120AOS  
Affinity Capture-MS Homo sapiens
110 IFIT1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 DHX40  
Affinity Capture-MS Homo sapiens
112 BAK1 578
Co-localization Homo sapiens
Protein-peptide Homo sapiens
113 C20orf195  
Affinity Capture-MS Homo sapiens
114 INF2 64423
Affinity Capture-MS Homo sapiens
115 GLI2  
Affinity Capture-MS Homo sapiens
116 VWA2 340706
Affinity Capture-MS Homo sapiens
117 RAPGEF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 DNM3 26052
Affinity Capture-MS Homo sapiens
119 CIT 11113
Affinity Capture-MS Homo sapiens
120 ARF1 375
Affinity Capture-Western Homo sapiens
121 IQGAP3 128239
Affinity Capture-MS Homo sapiens
122 FMO5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 RPA2 6118
Proximity Label-MS Homo sapiens
124 TRPC4AP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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